Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678 |
Resumo: | Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad. |
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Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)bromeliad; endangered species; ornamental plant; shooting; plant regeneration.bromeliad; endangered species; ornamental plant; shooting; plant regeneration.Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad.Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad.Universidade Estadual De Maringá2023-07-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6467810.4025/actascibiolsci.v45i1.64678Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e64678Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e646781807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678/751375156233Copyright (c) 2023 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTeixeira, Maria GessiNievola, Catarina Carvalho 2023-08-17T16:46:21Zoai:periodicos.uem.br/ojs:article/64678Revistahttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/PUBhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2023-08-17T16:46:21Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
title |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
spellingShingle |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) Teixeira, Maria Gessi bromeliad; endangered species; ornamental plant; shooting; plant regeneration. bromeliad; endangered species; ornamental plant; shooting; plant regeneration. |
title_short |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
title_full |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
title_fullStr |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
title_full_unstemmed |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
title_sort |
Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
author |
Teixeira, Maria Gessi |
author_facet |
Teixeira, Maria Gessi Nievola, Catarina Carvalho |
author_role |
author |
author2 |
Nievola, Catarina Carvalho |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Teixeira, Maria Gessi Nievola, Catarina Carvalho |
dc.subject.por.fl_str_mv |
bromeliad; endangered species; ornamental plant; shooting; plant regeneration. bromeliad; endangered species; ornamental plant; shooting; plant regeneration. |
topic |
bromeliad; endangered species; ornamental plant; shooting; plant regeneration. bromeliad; endangered species; ornamental plant; shooting; plant regeneration. |
description |
Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678 10.4025/actascibiolsci.v45i1.64678 |
url |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678 |
identifier_str_mv |
10.4025/actascibiolsci.v45i1.64678 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678/751375156233 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e64678 Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e64678 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317390426636288 |