Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827 |
Resumo: | The cyclodextrins (CDs) are cyclic oligosaccharides formed by residues of glucopyranose linked by α-1,4. The most common are the α-, β- and γ-CD that present 6, 7 and 8 units of glucopyranose, respectively. They are produced from starch by the action of the enzyme cyclodextrin glycosyltransferase (CGTase). Frequently, β-CD is produced in larger amount. A study of optimization of the production of the CGTase from Bacillus firmus, strain no. 37 (β-CGTase) was performed. The production of the enzyme occurred during the phase of exponential growth of the microorganism and the maximum activity was observed within three days of cultivation at 37ºC. The best production of the enzyme was obtained with inoculum of optical density between 0.5 and 1.0 (660 nm). The use of the maltodextrin for production of the enzyme provided an enzymatic activity at 31% lower than the substrate, soluble starch. Therefore, the substrate maltodextrin is not appropriate to improve the production of the studied enzyme. |
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Acta Scientiarum Biological Sciences |
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Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37Estudos de parâmetros que influenciam na produção da enzima CGTase de Bacillus firmus, cepa nº 37ciclodextrina glicosiltransferaseCGTaseciclodextrinas2.00.00.00-6 Ciências BiológicasThe cyclodextrins (CDs) are cyclic oligosaccharides formed by residues of glucopyranose linked by α-1,4. The most common are the α-, β- and γ-CD that present 6, 7 and 8 units of glucopyranose, respectively. They are produced from starch by the action of the enzyme cyclodextrin glycosyltransferase (CGTase). Frequently, β-CD is produced in larger amount. A study of optimization of the production of the CGTase from Bacillus firmus, strain no. 37 (β-CGTase) was performed. The production of the enzyme occurred during the phase of exponential growth of the microorganism and the maximum activity was observed within three days of cultivation at 37ºC. The best production of the enzyme was obtained with inoculum of optical density between 0.5 and 1.0 (660 nm). The use of the maltodextrin for production of the enzyme provided an enzymatic activity at 31% lower than the substrate, soluble starch. Therefore, the substrate maltodextrin is not appropriate to improve the production of the studied enzyme.As ciclodextrinas (CDs) são oligossacarídeos cíclicos formados por resíduos de glucopiranose unidos por ligação α-1,4. As mais comuns são α-, β- e γ-CD contendo 6, 7 e 8 resíduos de glucopiranose, respectivamente. Elas são produzidas a partir do amido pela ação da enzima ciclodextrina glicosiltransferase (CGTase). Freqüentemente, β-CD é produzida em maior quantidade. Um estudo da otimização da produção da CGTase de Bacillus firmus cepa nº 37 (β-CGTase) foi realizado. A produção da enzima ocorreu durante a fase de crescimento exponencial do microrganismo e a máxima atividade foi observada com três dias de cultivo a 37ºC. O melhor rendimento na produção da enzima foi obtido quando da utilização de pré-inóculo com absorbância entre 0,5 e 1,0 (660 nm). O uso do substrato maltodextrina para produção da enzima proporcionou uma atividade enzimática ao redor de 31% menor que o substrato amido solúvel. Portanto, o substrato maltodextrina não é adequado para melhorar a produção da enzima estudada.Universidade Estadual De Maringá2008-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/282710.4025/actascibiolsci.v22i0.2827Acta Scientiarum. Biological Sciences; Vol 22 (2000); 311-316Acta Scientiarum. Biological Sciences; v. 22 (2000); 311-3161807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827/2094Matioli, GracietteMoriwaki, CristianeMazzoni, Regiane BarbieriZanin, Gisella MariaMoraes, Flávio Faria deinfo:eu-repo/semantics/openAccess2022-11-23T17:33:30Zoai:periodicos.uem.br/ojs:article/2827Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:30Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 Estudos de parâmetros que influenciam na produção da enzima CGTase de Bacillus firmus, cepa nº 37 |
title |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 |
spellingShingle |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 Matioli, Graciette ciclodextrina glicosiltransferase CGTase ciclodextrinas 2.00.00.00-6 Ciências Biológicas |
title_short |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 |
title_full |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 |
title_fullStr |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 |
title_full_unstemmed |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 |
title_sort |
Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37 |
author |
Matioli, Graciette |
author_facet |
Matioli, Graciette Moriwaki, Cristiane Mazzoni, Regiane Barbieri Zanin, Gisella Maria Moraes, Flávio Faria de |
author_role |
author |
author2 |
Moriwaki, Cristiane Mazzoni, Regiane Barbieri Zanin, Gisella Maria Moraes, Flávio Faria de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Matioli, Graciette Moriwaki, Cristiane Mazzoni, Regiane Barbieri Zanin, Gisella Maria Moraes, Flávio Faria de |
dc.subject.por.fl_str_mv |
ciclodextrina glicosiltransferase CGTase ciclodextrinas 2.00.00.00-6 Ciências Biológicas |
topic |
ciclodextrina glicosiltransferase CGTase ciclodextrinas 2.00.00.00-6 Ciências Biológicas |
description |
The cyclodextrins (CDs) are cyclic oligosaccharides formed by residues of glucopyranose linked by α-1,4. The most common are the α-, β- and γ-CD that present 6, 7 and 8 units of glucopyranose, respectively. They are produced from starch by the action of the enzyme cyclodextrin glycosyltransferase (CGTase). Frequently, β-CD is produced in larger amount. A study of optimization of the production of the CGTase from Bacillus firmus, strain no. 37 (β-CGTase) was performed. The production of the enzyme occurred during the phase of exponential growth of the microorganism and the maximum activity was observed within three days of cultivation at 37ºC. The best production of the enzyme was obtained with inoculum of optical density between 0.5 and 1.0 (660 nm). The use of the maltodextrin for production of the enzyme provided an enzymatic activity at 31% lower than the substrate, soluble starch. Therefore, the substrate maltodextrin is not appropriate to improve the production of the studied enzyme. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-05-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827 10.4025/actascibiolsci.v22i0.2827 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827 |
identifier_str_mv |
10.4025/actascibiolsci.v22i0.2827 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827/2094 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 22 (2000); 311-316 Acta Scientiarum. Biological Sciences; v. 22 (2000); 311-316 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317390997061632 |