Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37

Detalhes bibliográficos
Autor(a) principal: Matioli, Graciette
Data de Publicação: 2008
Outros Autores: Moriwaki, Cristiane, Mazzoni, Regiane Barbieri, Zanin, Gisella Maria, Moraes, Flávio Faria de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827
Resumo: The cyclodextrins (CDs) are cyclic oligosaccharides formed by residues of glucopyranose linked by α-1,4. The most common are the α-, β- and γ-CD that present 6, 7 and 8 units of glucopyranose, respectively. They are produced from starch by the action of the enzyme cyclodextrin glycosyltransferase (CGTase). Frequently, β-CD is produced in larger amount. A study of optimization of the production of the CGTase from Bacillus firmus, strain no. 37 (β-CGTase) was performed. The production of the enzyme occurred during the phase of exponential growth of the microorganism and the maximum activity was observed within three days of cultivation at 37ºC. The best production of the enzyme was obtained with inoculum of optical density between 0.5 and 1.0 (660 nm). The use of the maltodextrin for production of the enzyme provided an enzymatic activity at 31% lower than the substrate, soluble starch. Therefore, the substrate maltodextrin is not appropriate to improve the production of the studied enzyme.
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spelling Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37Estudos de parâmetros que influenciam na produção da enzima CGTase de Bacillus firmus, cepa nº 37ciclodextrina glicosiltransferaseCGTaseciclodextrinas2.00.00.00-6 Ciências BiológicasThe cyclodextrins (CDs) are cyclic oligosaccharides formed by residues of glucopyranose linked by α-1,4. The most common are the α-, β- and γ-CD that present 6, 7 and 8 units of glucopyranose, respectively. They are produced from starch by the action of the enzyme cyclodextrin glycosyltransferase (CGTase). Frequently, β-CD is produced in larger amount. A study of optimization of the production of the CGTase from Bacillus firmus, strain no. 37 (β-CGTase) was performed. The production of the enzyme occurred during the phase of exponential growth of the microorganism and the maximum activity was observed within three days of cultivation at 37ºC. The best production of the enzyme was obtained with inoculum of optical density between 0.5 and 1.0 (660 nm). The use of the maltodextrin for production of the enzyme provided an enzymatic activity at 31% lower than the substrate, soluble starch. Therefore, the substrate maltodextrin is not appropriate to improve the production of the studied enzyme.As ciclodextrinas (CDs) são oligossacarídeos cíclicos formados por resíduos de glucopiranose unidos por ligação α-1,4. As mais comuns são α-, β- e γ-CD contendo 6, 7 e 8 resíduos de glucopiranose, respectivamente. Elas são produzidas a partir do amido pela ação da enzima ciclodextrina glicosiltransferase (CGTase). Freqüentemente, β-CD é produzida em maior quantidade. Um estudo da otimização da produção da CGTase de Bacillus firmus cepa nº 37 (β-CGTase) foi realizado. A produção da enzima ocorreu durante a fase de crescimento exponencial do microrganismo e a máxima atividade foi observada com três dias de cultivo a 37ºC. O melhor rendimento na produção da enzima foi obtido quando da utilização de pré-inóculo com absorbância entre 0,5 e 1,0 (660 nm). O uso do substrato maltodextrina para produção da enzima proporcionou uma atividade enzimática ao redor de 31% menor que o substrato amido solúvel. Portanto, o substrato maltodextrina não é adequado para melhorar a produção da enzima estudada.Universidade Estadual De Maringá2008-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/282710.4025/actascibiolsci.v22i0.2827Acta Scientiarum. Biological Sciences; Vol 22 (2000); 311-316Acta Scientiarum. Biological Sciences; v. 22 (2000); 311-3161807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827/2094Matioli, GracietteMoriwaki, CristianeMazzoni, Regiane BarbieriZanin, Gisella MariaMoraes, Flávio Faria deinfo:eu-repo/semantics/openAccess2022-11-23T17:33:30Zoai:periodicos.uem.br/ojs:article/2827Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:30Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
Estudos de parâmetros que influenciam na produção da enzima CGTase de Bacillus firmus, cepa nº 37
title Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
spellingShingle Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
Matioli, Graciette
ciclodextrina glicosiltransferase
CGTase
ciclodextrinas
2.00.00.00-6 Ciências Biológicas
title_short Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
title_full Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
title_fullStr Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
title_full_unstemmed Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
title_sort Study of parameters that influence the production of the enzyme CGTase from Bacillus firmus, strain no. 37
author Matioli, Graciette
author_facet Matioli, Graciette
Moriwaki, Cristiane
Mazzoni, Regiane Barbieri
Zanin, Gisella Maria
Moraes, Flávio Faria de
author_role author
author2 Moriwaki, Cristiane
Mazzoni, Regiane Barbieri
Zanin, Gisella Maria
Moraes, Flávio Faria de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Matioli, Graciette
Moriwaki, Cristiane
Mazzoni, Regiane Barbieri
Zanin, Gisella Maria
Moraes, Flávio Faria de
dc.subject.por.fl_str_mv ciclodextrina glicosiltransferase
CGTase
ciclodextrinas
2.00.00.00-6 Ciências Biológicas
topic ciclodextrina glicosiltransferase
CGTase
ciclodextrinas
2.00.00.00-6 Ciências Biológicas
description The cyclodextrins (CDs) are cyclic oligosaccharides formed by residues of glucopyranose linked by α-1,4. The most common are the α-, β- and γ-CD that present 6, 7 and 8 units of glucopyranose, respectively. They are produced from starch by the action of the enzyme cyclodextrin glycosyltransferase (CGTase). Frequently, β-CD is produced in larger amount. A study of optimization of the production of the CGTase from Bacillus firmus, strain no. 37 (β-CGTase) was performed. The production of the enzyme occurred during the phase of exponential growth of the microorganism and the maximum activity was observed within three days of cultivation at 37ºC. The best production of the enzyme was obtained with inoculum of optical density between 0.5 and 1.0 (660 nm). The use of the maltodextrin for production of the enzyme provided an enzymatic activity at 31% lower than the substrate, soluble starch. Therefore, the substrate maltodextrin is not appropriate to improve the production of the studied enzyme.
publishDate 2008
dc.date.none.fl_str_mv 2008-05-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827
10.4025/actascibiolsci.v22i0.2827
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827
identifier_str_mv 10.4025/actascibiolsci.v22i0.2827
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2827/2094
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 22 (2000); 311-316
Acta Scientiarum. Biological Sciences; v. 22 (2000); 311-316
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
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