Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419 |
Resumo: | The objective of this work was to evaluate the cytotoxic effect of the food dyes erythrosine, brilliant blue and red 40 on the cell cycle of Allium cepa L. Each dye was evaluated at doses of 0.4 and 4.0 ml, at exposure times of 24 and 48 hours, in onion root tip cells. Cells and the presence of chromosomal aberrations were analyzed throughout the whole cell cycle, totaling 5,000 cells for each group of bulbs. The mitotic index was calculated and the statistical analysis was conducted through the Chi-square test (p < 0.05). From the obtained results, it was verified that the food additives erythrosine and brilliant blue were not cytotoxic to the cells of the test system. However, the red 40 dye, at the two evaluated doses and the two exposure times used in this bioassay have promoted a significant reduction in cell division and induced the emergence of anaphasic and telophasic bridge aberrations and micronucleated cells. Additional cytotoxicity studies should be conducted to add information to these and other previously obtained results in order to evaluate, with property, the action of these three dyes on a cellular level. |
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Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419food additivedye toxicityAllium cepacitogenética The objective of this work was to evaluate the cytotoxic effect of the food dyes erythrosine, brilliant blue and red 40 on the cell cycle of Allium cepa L. Each dye was evaluated at doses of 0.4 and 4.0 ml, at exposure times of 24 and 48 hours, in onion root tip cells. Cells and the presence of chromosomal aberrations were analyzed throughout the whole cell cycle, totaling 5,000 cells for each group of bulbs. The mitotic index was calculated and the statistical analysis was conducted through the Chi-square test (p < 0.05). From the obtained results, it was verified that the food additives erythrosine and brilliant blue were not cytotoxic to the cells of the test system. However, the red 40 dye, at the two evaluated doses and the two exposure times used in this bioassay have promoted a significant reduction in cell division and induced the emergence of anaphasic and telophasic bridge aberrations and micronucleated cells. Additional cytotoxicity studies should be conducted to add information to these and other previously obtained results in order to evaluate, with property, the action of these three dyes on a cellular level. Universidade Estadual De Maringá2013-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioncontagem e observação celularapplication/pdfapplication/pdfapplication/mswordhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1841910.4025/actascibiolsci.v35i4.18419Acta Scientiarum. Biological Sciences; Vol 35 No 4 (2013); 557-562Acta Scientiarum. Biological Sciences; v. 35 n. 4 (2013); 557-5621807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419/pdf_1http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419/751375141780Oliveira, Maria Virna Aguiar deAlves, Dandará Darhlen LopesLima, Leonardo Henrique Guedes de MoraisSousa, João Marcelo de Castro ePeron, Ana Paulainfo:eu-repo/semantics/openAccess2022-11-23T17:34:11Zoai:periodicos.uem.br/ojs:article/18419Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:34:11Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 |
title |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 |
spellingShingle |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 Oliveira, Maria Virna Aguiar de food additive dye toxicity Allium cepa citogenética |
title_short |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 |
title_full |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 |
title_fullStr |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 |
title_full_unstemmed |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 |
title_sort |
Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system - doi: 10.4025/actascibiolsci.v35i4.18419 |
author |
Oliveira, Maria Virna Aguiar de |
author_facet |
Oliveira, Maria Virna Aguiar de Alves, Dandará Darhlen Lopes Lima, Leonardo Henrique Guedes de Morais Sousa, João Marcelo de Castro e Peron, Ana Paula |
author_role |
author |
author2 |
Alves, Dandará Darhlen Lopes Lima, Leonardo Henrique Guedes de Morais Sousa, João Marcelo de Castro e Peron, Ana Paula |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Maria Virna Aguiar de Alves, Dandará Darhlen Lopes Lima, Leonardo Henrique Guedes de Morais Sousa, João Marcelo de Castro e Peron, Ana Paula |
dc.subject.por.fl_str_mv |
food additive dye toxicity Allium cepa citogenética |
topic |
food additive dye toxicity Allium cepa citogenética |
description |
The objective of this work was to evaluate the cytotoxic effect of the food dyes erythrosine, brilliant blue and red 40 on the cell cycle of Allium cepa L. Each dye was evaluated at doses of 0.4 and 4.0 ml, at exposure times of 24 and 48 hours, in onion root tip cells. Cells and the presence of chromosomal aberrations were analyzed throughout the whole cell cycle, totaling 5,000 cells for each group of bulbs. The mitotic index was calculated and the statistical analysis was conducted through the Chi-square test (p < 0.05). From the obtained results, it was verified that the food additives erythrosine and brilliant blue were not cytotoxic to the cells of the test system. However, the red 40 dye, at the two evaluated doses and the two exposure times used in this bioassay have promoted a significant reduction in cell division and induced the emergence of anaphasic and telophasic bridge aberrations and micronucleated cells. Additional cytotoxicity studies should be conducted to add information to these and other previously obtained results in order to evaluate, with property, the action of these three dyes on a cellular level. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion contagem e observação celular |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419 10.4025/actascibiolsci.v35i4.18419 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419 |
identifier_str_mv |
10.4025/actascibiolsci.v35i4.18419 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419/pdf_1 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18419/751375141780 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/msword |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 35 No 4 (2013); 557-562 Acta Scientiarum. Biological Sciences; v. 35 n. 4 (2013); 557-562 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317395885522944 |