Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae

Detalhes bibliográficos
Autor(a) principal: Jacik, Stefhani Cristina Chan
Data de Publicação: 2023
Outros Autores: Aquino, Julia Bastos de, Melo, Luana Felix de, Rodrigues, Rosângela Felipe, Melo, Alan Peres Feraz de, Miglino, Maria Angélica, Rici, Rose Eli Grassi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/63722
Resumo: The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. The objective of this research was to analyze the morphological aspects and germ cells of the male reproductive tract of Potamotrygon amandae. The samples were fixed in 10% formalin, and then dehydrated in an ascending ethanol series (70 to 100%). To carry out light microscopy analyses, they were embedded in paraffin, cut and stained; as for scanning electron microscopy analyses, the samples were dried, glued in metallic bases and metalized. The gross morphology consisted of the following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, and the clasper gland. Microscopically, several stages of spermatogenesis were observed in the testis, occurring in spherical follicles, similar to other stingrays. The epididymis was formed by one duct subdivided in various tubules. The deferent ducts were continuous with the epididymis, and the lumen was full of spermatozoa. The Leydig glands consisted of glandular units with eosinophilic content in the lumen of some, and the deferent ducts ran parallel to the ventral portion. The seminal vesicles possessed numerous compartments to store the sperm, with a wall similar to a hive, and the lumen was full of spermatozoa. Alcian Blue (AB) and Periodic Schiff-Acid (PAS) performed in the Leydig Gland, deferens ducts and seminal vesicle was positive only in the connective tissue, the cilia were PAS+ and the nuclei stained weakly for AB. The clasper gland was composed of unit glands and was covered with striated muscle externally. It stained very well with Periodic Schiff-Acid. The morphological aspects of the male reproductive tract of Potamotrygon amandae were similar to other stingrays.
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spelling Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandaeMorphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandaeelasmobranchs; freshwater stingray; masculine tract; germ cells.elasmobranchs; freshwater stingray; masculine tract; germ cells.The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. The objective of this research was to analyze the morphological aspects and germ cells of the male reproductive tract of Potamotrygon amandae. The samples were fixed in 10% formalin, and then dehydrated in an ascending ethanol series (70 to 100%). To carry out light microscopy analyses, they were embedded in paraffin, cut and stained; as for scanning electron microscopy analyses, the samples were dried, glued in metallic bases and metalized. The gross morphology consisted of the following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, and the clasper gland. Microscopically, several stages of spermatogenesis were observed in the testis, occurring in spherical follicles, similar to other stingrays. The epididymis was formed by one duct subdivided in various tubules. The deferent ducts were continuous with the epididymis, and the lumen was full of spermatozoa. The Leydig glands consisted of glandular units with eosinophilic content in the lumen of some, and the deferent ducts ran parallel to the ventral portion. The seminal vesicles possessed numerous compartments to store the sperm, with a wall similar to a hive, and the lumen was full of spermatozoa. Alcian Blue (AB) and Periodic Schiff-Acid (PAS) performed in the Leydig Gland, deferens ducts and seminal vesicle was positive only in the connective tissue, the cilia were PAS+ and the nuclei stained weakly for AB. The clasper gland was composed of unit glands and was covered with striated muscle externally. It stained very well with Periodic Schiff-Acid. The morphological aspects of the male reproductive tract of Potamotrygon amandae were similar to other stingrays.The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. The objective of this research was to analyze the morphological aspects and germ cells of the male reproductive tract of Potamotrygon amandae. The samples were fixed in 10% formalin, and then dehydrated in an ascending ethanol series (70 to 100%). To carry out light microscopy analyses, they were embedded in paraffin, cut and stained; as for scanning electron microscopy analyses, the samples were dried, glued in metallic bases and metalized. The gross morphology consisted of the following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, and the clasper gland. Microscopically, several stages of spermatogenesis were observed in the testis, occurring in spherical follicles, similar to other stingrays. The epididymis was formed by one duct subdivided in various tubules. The deferent ducts were continuous with the epididymis, and the lumen was full of spermatozoa. The Leydig glands consisted of glandular units with eosinophilic content in the lumen of some, and the deferent ducts ran parallel to the ventral portion. The seminal vesicles possessed numerous compartments to store the sperm, with a wall similar to a hive, and the lumen was full of spermatozoa. Alcian Blue (AB) and Periodic Schiff-Acid (PAS) performed in the Leydig Gland, deferens ducts and seminal vesicle was positive only in the connective tissue, the cilia were PAS+ and the nuclei stained weakly for AB. The clasper gland was composed of unit glands and was covered with striated muscle externally. It stained very well with Periodic Schiff-Acid. The morphological aspects of the male reproductive tract of Potamotrygon amandae were similar to other stingrays.Universidade Estadual De Maringá2023-04-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6372210.4025/actascibiolsci.v45i1.63722Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e63722Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e637221807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/63722/751375155797Copyright (c) 2023 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessJacik, Stefhani Cristina Chan Aquino, Julia Bastos de Melo, Luana Felix deRodrigues, Rosângela Felipe Melo, Alan Peres Feraz de Miglino, Maria Angélica Rici, Rose Eli Grassi 2023-05-25T13:46:36Zoai:periodicos.uem.br/ojs:article/63722Revistahttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/PUBhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2023-05-25T13:46:36Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
title Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
spellingShingle Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
Jacik, Stefhani Cristina Chan
elasmobranchs; freshwater stingray; masculine tract; germ cells.
elasmobranchs; freshwater stingray; masculine tract; germ cells.
title_short Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
title_full Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
title_fullStr Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
title_full_unstemmed Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
title_sort Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae
author Jacik, Stefhani Cristina Chan
author_facet Jacik, Stefhani Cristina Chan
Aquino, Julia Bastos de
Melo, Luana Felix de
Rodrigues, Rosângela Felipe
Melo, Alan Peres Feraz de
Miglino, Maria Angélica
Rici, Rose Eli Grassi
author_role author
author2 Aquino, Julia Bastos de
Melo, Luana Felix de
Rodrigues, Rosângela Felipe
Melo, Alan Peres Feraz de
Miglino, Maria Angélica
Rici, Rose Eli Grassi
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jacik, Stefhani Cristina Chan
Aquino, Julia Bastos de
Melo, Luana Felix de
Rodrigues, Rosângela Felipe
Melo, Alan Peres Feraz de
Miglino, Maria Angélica
Rici, Rose Eli Grassi
dc.subject.por.fl_str_mv elasmobranchs; freshwater stingray; masculine tract; germ cells.
elasmobranchs; freshwater stingray; masculine tract; germ cells.
topic elasmobranchs; freshwater stingray; masculine tract; germ cells.
elasmobranchs; freshwater stingray; masculine tract; germ cells.
description The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. The objective of this research was to analyze the morphological aspects and germ cells of the male reproductive tract of Potamotrygon amandae. The samples were fixed in 10% formalin, and then dehydrated in an ascending ethanol series (70 to 100%). To carry out light microscopy analyses, they were embedded in paraffin, cut and stained; as for scanning electron microscopy analyses, the samples were dried, glued in metallic bases and metalized. The gross morphology consisted of the following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, and the clasper gland. Microscopically, several stages of spermatogenesis were observed in the testis, occurring in spherical follicles, similar to other stingrays. The epididymis was formed by one duct subdivided in various tubules. The deferent ducts were continuous with the epididymis, and the lumen was full of spermatozoa. The Leydig glands consisted of glandular units with eosinophilic content in the lumen of some, and the deferent ducts ran parallel to the ventral portion. The seminal vesicles possessed numerous compartments to store the sperm, with a wall similar to a hive, and the lumen was full of spermatozoa. Alcian Blue (AB) and Periodic Schiff-Acid (PAS) performed in the Leydig Gland, deferens ducts and seminal vesicle was positive only in the connective tissue, the cilia were PAS+ and the nuclei stained weakly for AB. The clasper gland was composed of unit glands and was covered with striated muscle externally. It stained very well with Periodic Schiff-Acid. The morphological aspects of the male reproductive tract of Potamotrygon amandae were similar to other stingrays.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/63722
10.4025/actascibiolsci.v45i1.63722
url https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/63722
identifier_str_mv 10.4025/actascibiolsci.v45i1.63722
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/63722/751375155797
dc.rights.driver.fl_str_mv Copyright (c) 2023 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e63722
Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e63722
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
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instname_str Universidade Estadual de Maringá (UEM)
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collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
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