Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093

Detalhes bibliográficos
Autor(a) principal: Faleiro, Alexandro Cezar
Data de Publicação: 2008
Outros Autores: Resende, Adriana Gazoli, Machado, Maria de Fátima Pires da Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2093
Resumo: Malate dehydrogenase (MDH) isozymes were used as biochemical markers to discriminate and cluster cladophylls of plants of one clonal population of the prickly pear, Opuntia ficus-indica (Cactaceae). The isozyme electrophoretic patterns obtained with MDH provided 8 isozymes and 5 different electrophoretic phenotypes. Similarity in cladophylls was estimated using Jaccard’s coefficient. This clonal population studied was founded by only one propagule, and after 50 years, it is likely to have been formed by asexual and sexual propagules. Since that differential expression of MDH isozymes could play a significant role in overall plant cell metabolism, we suggest that the cladophylls of prickly pear that were clustered together showing identity or higher similarity are specially a suitable source for industrial procedures of industrial extraction of commercial interest compounds because a same extraction protocols can be most quickly and easily standardized using genetically uniform materials. Electrophoretic patterns of MDH isozymes can be used as an effective tool for previously determine the genetic similarity in the cladophylls of O. ficus-indica plants
id UEM-1_5deae6e9d48f3ff0470c6d09bb812397
oai_identifier_str oai:periodicos.uem.br/ojs:article/2093
network_acronym_str UEM-1
network_name_str Acta Scientiarum Biological Sciences
repository_id_str
spelling Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093Padrões de isozimas de malato desidrogenase em população clonal nos cladófilos de Opuntia ficus-indica Mill (Cactaceae) - DOI: 10.4025/actascibiolsci.v25i1.2093isozimasmalato desidrogenasepalmapolimorfismosimilaridade genética2.01.00.00-0 Biologia GeralMalate dehydrogenase (MDH) isozymes were used as biochemical markers to discriminate and cluster cladophylls of plants of one clonal population of the prickly pear, Opuntia ficus-indica (Cactaceae). The isozyme electrophoretic patterns obtained with MDH provided 8 isozymes and 5 different electrophoretic phenotypes. Similarity in cladophylls was estimated using Jaccard’s coefficient. This clonal population studied was founded by only one propagule, and after 50 years, it is likely to have been formed by asexual and sexual propagules. Since that differential expression of MDH isozymes could play a significant role in overall plant cell metabolism, we suggest that the cladophylls of prickly pear that were clustered together showing identity or higher similarity are specially a suitable source for industrial procedures of industrial extraction of commercial interest compounds because a same extraction protocols can be most quickly and easily standardized using genetically uniform materials. Electrophoretic patterns of MDH isozymes can be used as an effective tool for previously determine the genetic similarity in the cladophylls of O. ficus-indica plantsIsozimas de malato desidrogenase (MDH) foram usadas como marcadores moleculares para discriminar e agrupar cladófilos de plantas de uma população clonal de cactus da espécie Opuntia ficus-indica (Cactaceae), conhecida como palma. O padrão eletroforético obtido revelou 8 isozimas MDH e 5 fenótipos eletroforéticos diferentes. A similaridade entre os cladófilos foi estimada usando o coeficiente de similaridade de Jaccard. Essa população clonal estudada foi fundada por somente um propágulo e, após 50 anos, parece ser formada por propágulos assexuais e sexuais. Uma vez que a expressão diferencial de isozimas MDH pode ter um papel significante no metabolismo das células da planta, sugerimos que os cladófilos de palma que foram agrupados com os mais altos valores de similaridade são os mais adequados para serem utilizados em procedimentos de extração industrial de compostos de interesse comercial, porque um mesmo protocolo de extração pode ser mais rapidamente e facilmente padronizado quando se utiliza material geneticamente uniforme. O padrão eletroforético das isozimas MDH pode ser usado como uma ferramenta efetiva para uma análise prévia da similaridade genética entre os cladófilos das plantas de O. ficus-indicaUniversidade Estadual De Maringá2008-04-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/209310.4025/actascibiolsci.v25i1.2093Acta Scientiarum. Biological Sciences; Vol 25 No 1 (2003); 207-211Acta Scientiarum. Biological Sciences; v. 25 n. 1 (2003); 207-2111807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2093/2093Faleiro, Alexandro CezarResende, Adriana GazoliMachado, Maria de Fátima Pires da Silvainfo:eu-repo/semantics/openAccess2022-11-23T17:33:20Zoai:periodicos.uem.br/ojs:article/2093Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:20Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
Padrões de isozimas de malato desidrogenase em população clonal nos cladófilos de Opuntia ficus-indica Mill (Cactaceae) - DOI: 10.4025/actascibiolsci.v25i1.2093
title Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
spellingShingle Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
Faleiro, Alexandro Cezar
isozimas
malato desidrogenase
palma
polimorfismo
similaridade genética
2.01.00.00-0 Biologia Geral
title_short Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
title_full Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
title_fullStr Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
title_full_unstemmed Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
title_sort Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093
author Faleiro, Alexandro Cezar
author_facet Faleiro, Alexandro Cezar
Resende, Adriana Gazoli
Machado, Maria de Fátima Pires da Silva
author_role author
author2 Resende, Adriana Gazoli
Machado, Maria de Fátima Pires da Silva
author2_role author
author
dc.contributor.author.fl_str_mv Faleiro, Alexandro Cezar
Resende, Adriana Gazoli
Machado, Maria de Fátima Pires da Silva
dc.subject.por.fl_str_mv isozimas
malato desidrogenase
palma
polimorfismo
similaridade genética
2.01.00.00-0 Biologia Geral
topic isozimas
malato desidrogenase
palma
polimorfismo
similaridade genética
2.01.00.00-0 Biologia Geral
description Malate dehydrogenase (MDH) isozymes were used as biochemical markers to discriminate and cluster cladophylls of plants of one clonal population of the prickly pear, Opuntia ficus-indica (Cactaceae). The isozyme electrophoretic patterns obtained with MDH provided 8 isozymes and 5 different electrophoretic phenotypes. Similarity in cladophylls was estimated using Jaccard’s coefficient. This clonal population studied was founded by only one propagule, and after 50 years, it is likely to have been formed by asexual and sexual propagules. Since that differential expression of MDH isozymes could play a significant role in overall plant cell metabolism, we suggest that the cladophylls of prickly pear that were clustered together showing identity or higher similarity are specially a suitable source for industrial procedures of industrial extraction of commercial interest compounds because a same extraction protocols can be most quickly and easily standardized using genetically uniform materials. Electrophoretic patterns of MDH isozymes can be used as an effective tool for previously determine the genetic similarity in the cladophylls of O. ficus-indica plants
publishDate 2008
dc.date.none.fl_str_mv 2008-04-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2093
10.4025/actascibiolsci.v25i1.2093
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2093
identifier_str_mv 10.4025/actascibiolsci.v25i1.2093
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2093/2093
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 25 No 1 (2003); 207-211
Acta Scientiarum. Biological Sciences; v. 25 n. 1 (2003); 207-211
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
_version_ 1799317392972578816

Registros relacionados