Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis

Detalhes bibliográficos
Autor(a) principal: Chiari, Lucimara
Data de Publicação: 2008
Outros Autores: Sodré, Leda Maria Koelblinger
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2699
Resumo: Random Amplified Polymorphic DNA (RAPD) techniques were applied to eight Anostomidae species to analyze the genetic variability and genetic similarity within and between species. The results obtained were compared to those from isoenzyme analysis executed for many of the species studied. The proportion of polymorphic loci obtained with 10 primers was over 40%, with the exception of Leporinus amblyhynchus. This is especially important since genetic variability is a population feature both for short term fitness of the individual and for the long term survival of populations. Through subsequent analysis of dendrograms it was possible to discriminate the two Schizodon species from those of the genus Leporinus, even though it was impossible to differentiate L. obtusidens and L. elongates. RAPD analysis indicates that the two species are genetically very similar, supported too by isoenzyme data
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spelling Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysisEstudo de oito espécies da familia Anostomidae (Pisces, Characiformes) através da análise de RAPDDNApolimorfismovariabilidade genéticaRAPDAnostomidaepeixe2.00.00.00-6 Ciências BiológicasRandom Amplified Polymorphic DNA (RAPD) techniques were applied to eight Anostomidae species to analyze the genetic variability and genetic similarity within and between species. The results obtained were compared to those from isoenzyme analysis executed for many of the species studied. The proportion of polymorphic loci obtained with 10 primers was over 40%, with the exception of Leporinus amblyhynchus. This is especially important since genetic variability is a population feature both for short term fitness of the individual and for the long term survival of populations. Through subsequent analysis of dendrograms it was possible to discriminate the two Schizodon species from those of the genus Leporinus, even though it was impossible to differentiate L. obtusidens and L. elongates. RAPD analysis indicates that the two species are genetically very similar, supported too by isoenzyme dataA técnica de Polimorfismo de DNA Amplificado ao Acaso (RAPD) foi utilizada na análise de oito espécies de peixe da família Anostomidae, com a finalidade de quantificar a variabilidade genética e estimar a similaridade genética dentro e entre essas espécies. Os resultados foram comparados com dados obtidos anteriormente com isoenzimas, para a maioria das espécies analisadas. A proporção de locos polimórficos obtida, com dez primers, está acima de 40 %, exceto para Leporinus amblyhynchus. Esse é um fato importante desde que a existência de variabilidade genética é uma condição importante para a sobrevida das espécies no meio ambiente Com dendrograma obtido, através de análise comparativa, foi possível discriminar as duas espécies de Schizodon daquelas do gênero Leporinus, no entanto não foi possível separar L. obtusidens e L. elongatus. A análise de RAPD indica que essas duas espécies são genéticamante muito similares, conclusão também sustentada por dados de estudos de isoenzimasUniversidade Estadual De Maringá2008-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/269910.4025/actascibiolsci.v23i0.2699Acta Scientiarum. Biological Sciences; Vol 23 (2001); 445-451Acta Scientiarum. Biological Sciences; v. 23 (2001); 445-4511807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2699/2017Chiari, LucimaraSodré, Leda Maria Koelblingerinfo:eu-repo/semantics/openAccess2022-11-23T17:33:27Zoai:periodicos.uem.br/ojs:article/2699Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:27Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
Estudo de oito espécies da familia Anostomidae (Pisces, Characiformes) através da análise de RAPD
title Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
spellingShingle Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
Chiari, Lucimara
DNA
polimorfismo
variabilidade genética
RAPD
Anostomidae
peixe
2.00.00.00-6 Ciências Biológicas
title_short Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
title_full Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
title_fullStr Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
title_full_unstemmed Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
title_sort Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis
author Chiari, Lucimara
author_facet Chiari, Lucimara
Sodré, Leda Maria Koelblinger
author_role author
author2 Sodré, Leda Maria Koelblinger
author2_role author
dc.contributor.author.fl_str_mv Chiari, Lucimara
Sodré, Leda Maria Koelblinger
dc.subject.por.fl_str_mv DNA
polimorfismo
variabilidade genética
RAPD
Anostomidae
peixe
2.00.00.00-6 Ciências Biológicas
topic DNA
polimorfismo
variabilidade genética
RAPD
Anostomidae
peixe
2.00.00.00-6 Ciências Biológicas
description Random Amplified Polymorphic DNA (RAPD) techniques were applied to eight Anostomidae species to analyze the genetic variability and genetic similarity within and between species. The results obtained were compared to those from isoenzyme analysis executed for many of the species studied. The proportion of polymorphic loci obtained with 10 primers was over 40%, with the exception of Leporinus amblyhynchus. This is especially important since genetic variability is a population feature both for short term fitness of the individual and for the long term survival of populations. Through subsequent analysis of dendrograms it was possible to discriminate the two Schizodon species from those of the genus Leporinus, even though it was impossible to differentiate L. obtusidens and L. elongates. RAPD analysis indicates that the two species are genetically very similar, supported too by isoenzyme data
publishDate 2008
dc.date.none.fl_str_mv 2008-05-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2699
10.4025/actascibiolsci.v23i0.2699
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2699
identifier_str_mv 10.4025/actascibiolsci.v23i0.2699
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2699/2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 23 (2001); 445-451
Acta Scientiarum. Biological Sciences; v. 23 (2001); 445-451
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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