DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33024 |
Resumo: | Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus. |
id |
UEM-1_7111793efcae3e3512afd9853f58b098 |
---|---|
oai_identifier_str |
oai:periodicos.uem.br/ojs:article/33024 |
network_acronym_str |
UEM-1 |
network_name_str |
Acta Scientiarum Biological Sciences |
repository_id_str |
|
spelling |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesiiorchid improvementgenetic variationspecies separation.2.02.03.00-4 Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus. Universidade Estadual De Maringá2017-05-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3302410.4025/actascibiolsci.v39i1.33024Acta Scientiarum. Biological Sciences; Vol 39 No 1 (2017); 45-52Acta Scientiarum. Biological Sciences; v. 39 n. 1 (2017); 45-521807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33024/pdfCopyright (c) 2017 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessRivera-Jiménez, HernandoRossini, Bruno CésarTambarussi, Evandro VagnerAnn Veasey, ElizabethIbanes, BrunaMarino, Celso Luis2022-02-20T22:00:10Zoai:periodicos.uem.br/ojs:article/33024Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-20T22:00:10Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
spellingShingle |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii Rivera-Jiménez, Hernando orchid improvement genetic variation species separation. 2.02.03.00-4 |
title_short |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_full |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_fullStr |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_full_unstemmed |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_sort |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
author |
Rivera-Jiménez, Hernando |
author_facet |
Rivera-Jiménez, Hernando Rossini, Bruno César Tambarussi, Evandro Vagner Ann Veasey, Elizabeth Ibanes, Bruna Marino, Celso Luis |
author_role |
author |
author2 |
Rossini, Bruno César Tambarussi, Evandro Vagner Ann Veasey, Elizabeth Ibanes, Bruna Marino, Celso Luis |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rivera-Jiménez, Hernando Rossini, Bruno César Tambarussi, Evandro Vagner Ann Veasey, Elizabeth Ibanes, Bruna Marino, Celso Luis |
dc.subject.por.fl_str_mv |
orchid improvement genetic variation species separation. 2.02.03.00-4 |
topic |
orchid improvement genetic variation species separation. 2.02.03.00-4 |
description |
Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33024 10.4025/actascibiolsci.v39i1.33024 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33024 |
identifier_str_mv |
10.4025/actascibiolsci.v39i1.33024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33024/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 39 No 1 (2017); 45-52 Acta Scientiarum. Biological Sciences; v. 39 n. 1 (2017); 45-52 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317396545077248 |