Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23483 |
Resumo: | Studies on new microbial sources of cellulase and accurate assessment of the steps that increase cellulase production are essential strategies to reduce costs of various processes using such enzymes. This study aimed at the selection of cellulase-producing filamentous fungi, and at the research of parameters involving cellulase production by submerged fermentation. The first test consisted of selecting the best cellulase-producing microorganisms (FPase) in Erlenmeyer flasks containing 200 mL of specific growth medium. The next test was designed to further investigate the enzyme production in fermentation with four types of soluble sugars: glucose, lactose, sucrose and xylose. In bioreactor tests, three different inoculation strategies were analyzed. The best FPase activity was presented by the strain Trichoderma sp. CMIAT 041 (49.9 FPU L-1) and CMCase by the fungus Lasiodiplodia theobromae CMIAT 096 (350.0 U L-1). Sucrose proved to be the best option among the soluble sugars tested, with higher rates of FPase activity (49.9 FPU L-1) and CMCase (119.7 U L-1). The best inoculation strategy for the bioreactor was a spore suspension obtained from a semi-solid state fermentation of wheat bran for 72h. |
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Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biomecellulolytic enzymesenzyme inductionMicrobiologia Industrial e de FermentaçãoStudies on new microbial sources of cellulase and accurate assessment of the steps that increase cellulase production are essential strategies to reduce costs of various processes using such enzymes. This study aimed at the selection of cellulase-producing filamentous fungi, and at the research of parameters involving cellulase production by submerged fermentation. The first test consisted of selecting the best cellulase-producing microorganisms (FPase) in Erlenmeyer flasks containing 200 mL of specific growth medium. The next test was designed to further investigate the enzyme production in fermentation with four types of soluble sugars: glucose, lactose, sucrose and xylose. In bioreactor tests, three different inoculation strategies were analyzed. The best FPase activity was presented by the strain Trichoderma sp. CMIAT 041 (49.9 FPU L-1) and CMCase by the fungus Lasiodiplodia theobromae CMIAT 096 (350.0 U L-1). Sucrose proved to be the best option among the soluble sugars tested, with higher rates of FPase activity (49.9 FPU L-1) and CMCase (119.7 U L-1). The best inoculation strategy for the bioreactor was a spore suspension obtained from a semi-solid state fermentation of wheat bran for 72h. Universidade Estadual De Maringá2015-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2348310.4025/actascibiolsci.v37i1.23483Acta Scientiarum. Biological Sciences; Vol 37 No 1 (2015); 15-22Acta Scientiarum. Biological Sciences; v. 37 n. 1 (2015); 15-221807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23483/pdf_34Faheina Junior, Genilton da SilvaAmorim, Mariza Vieira da Fonseca SabóiaSouza, Caroline Gondim deSousa, Diego Menezes deSousa, Kally Alves dePinto, Gustavo Adolfo Saavedrainfo:eu-repo/semantics/openAccess2015-03-10T09:15:09Zoai:periodicos.uem.br/ojs:article/23483Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2015-03-10T09:15:09Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
title |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
spellingShingle |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome Faheina Junior, Genilton da Silva cellulolytic enzymes enzyme induction Microbiologia Industrial e de Fermentação |
title_short |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
title_full |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
title_fullStr |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
title_full_unstemmed |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
title_sort |
Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
author |
Faheina Junior, Genilton da Silva |
author_facet |
Faheina Junior, Genilton da Silva Amorim, Mariza Vieira da Fonseca Sabóia Souza, Caroline Gondim de Sousa, Diego Menezes de Sousa, Kally Alves de Pinto, Gustavo Adolfo Saavedra |
author_role |
author |
author2 |
Amorim, Mariza Vieira da Fonseca Sabóia Souza, Caroline Gondim de Sousa, Diego Menezes de Sousa, Kally Alves de Pinto, Gustavo Adolfo Saavedra |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Faheina Junior, Genilton da Silva Amorim, Mariza Vieira da Fonseca Sabóia Souza, Caroline Gondim de Sousa, Diego Menezes de Sousa, Kally Alves de Pinto, Gustavo Adolfo Saavedra |
dc.subject.por.fl_str_mv |
cellulolytic enzymes enzyme induction Microbiologia Industrial e de Fermentação |
topic |
cellulolytic enzymes enzyme induction Microbiologia Industrial e de Fermentação |
description |
Studies on new microbial sources of cellulase and accurate assessment of the steps that increase cellulase production are essential strategies to reduce costs of various processes using such enzymes. This study aimed at the selection of cellulase-producing filamentous fungi, and at the research of parameters involving cellulase production by submerged fermentation. The first test consisted of selecting the best cellulase-producing microorganisms (FPase) in Erlenmeyer flasks containing 200 mL of specific growth medium. The next test was designed to further investigate the enzyme production in fermentation with four types of soluble sugars: glucose, lactose, sucrose and xylose. In bioreactor tests, three different inoculation strategies were analyzed. The best FPase activity was presented by the strain Trichoderma sp. CMIAT 041 (49.9 FPU L-1) and CMCase by the fungus Lasiodiplodia theobromae CMIAT 096 (350.0 U L-1). Sucrose proved to be the best option among the soluble sugars tested, with higher rates of FPase activity (49.9 FPU L-1) and CMCase (119.7 U L-1). The best inoculation strategy for the bioreactor was a spore suspension obtained from a semi-solid state fermentation of wheat bran for 72h. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23483 10.4025/actascibiolsci.v37i1.23483 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23483 |
identifier_str_mv |
10.4025/actascibiolsci.v37i1.23483 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23483/pdf_34 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 37 No 1 (2015); 15-22 Acta Scientiarum. Biological Sciences; v. 37 n. 1 (2015); 15-22 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317396029177856 |