Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay

Detalhes bibliográficos
Autor(a) principal: Cosme, Marcos Vinícius
Data de Publicação: 2022
Outros Autores: Nunes, Nárcia Mariana Fonseca, Oliveira, Aldeídia Pereira de, Oliveira, Elisângela Cláudia Alves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665
Resumo: The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.
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spelling Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassayEvaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassayAllium cepa; cytotoxicity; genotoxicity; mutagenesis.Allium cepa; cytotoxicity; genotoxicity; mutagenesis.The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.Universidade Estadual De Maringá2022-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6266510.4025/actascibiolsci.v44i1.62665Acta Scientiarum. Biological Sciences; Vol 44 (2022): Publicação contínua; e62665Acta Scientiarum. Biological Sciences; v. 44 (2022): Publicação contínua; e626651807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665/751375154969Copyright (c) 2022 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosme, Marcos ViníciusNunes, Nárcia Mariana Fonseca Oliveira, Aldeídia Pereira de Oliveira, Elisângela Cláudia Alves de2022-11-22T18:57:09Zoai:periodicos.uem.br/ojs:article/62665Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-22T18:57:09Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
title Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
spellingShingle Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
Cosme, Marcos Vinícius
Allium cepa; cytotoxicity; genotoxicity; mutagenesis.
Allium cepa; cytotoxicity; genotoxicity; mutagenesis.
title_short Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
title_full Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
title_fullStr Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
title_full_unstemmed Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
title_sort Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
author Cosme, Marcos Vinícius
author_facet Cosme, Marcos Vinícius
Nunes, Nárcia Mariana Fonseca
Oliveira, Aldeídia Pereira de
Oliveira, Elisângela Cláudia Alves de
author_role author
author2 Nunes, Nárcia Mariana Fonseca
Oliveira, Aldeídia Pereira de
Oliveira, Elisângela Cláudia Alves de
author2_role author
author
author
dc.contributor.author.fl_str_mv Cosme, Marcos Vinícius
Nunes, Nárcia Mariana Fonseca
Oliveira, Aldeídia Pereira de
Oliveira, Elisângela Cláudia Alves de
dc.subject.por.fl_str_mv Allium cepa; cytotoxicity; genotoxicity; mutagenesis.
Allium cepa; cytotoxicity; genotoxicity; mutagenesis.
topic Allium cepa; cytotoxicity; genotoxicity; mutagenesis.
Allium cepa; cytotoxicity; genotoxicity; mutagenesis.
description The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665
10.4025/actascibiolsci.v44i1.62665
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665
identifier_str_mv 10.4025/actascibiolsci.v44i1.62665
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665/751375154969
dc.rights.driver.fl_str_mv Copyright (c) 2022 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 44 (2022): Publicação contínua; e62665
Acta Scientiarum. Biological Sciences; v. 44 (2022): Publicação contínua; e62665
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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