Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665 |
Resumo: | The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action. |
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Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassayEvaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassayAllium cepa; cytotoxicity; genotoxicity; mutagenesis.Allium cepa; cytotoxicity; genotoxicity; mutagenesis.The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.Universidade Estadual De Maringá2022-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6266510.4025/actascibiolsci.v44i1.62665Acta Scientiarum. Biological Sciences; Vol 44 (2022): Publicação contínua; e62665Acta Scientiarum. Biological Sciences; v. 44 (2022): Publicação contínua; e626651807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665/751375154969Copyright (c) 2022 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosme, Marcos ViníciusNunes, Nárcia Mariana Fonseca Oliveira, Aldeídia Pereira de Oliveira, Elisângela Cláudia Alves de2022-11-22T18:57:09Zoai:periodicos.uem.br/ojs:article/62665Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-22T18:57:09Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay |
title |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay |
spellingShingle |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay Cosme, Marcos Vinícius Allium cepa; cytotoxicity; genotoxicity; mutagenesis. Allium cepa; cytotoxicity; genotoxicity; mutagenesis. |
title_short |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay |
title_full |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay |
title_fullStr |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay |
title_full_unstemmed |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay |
title_sort |
Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay |
author |
Cosme, Marcos Vinícius |
author_facet |
Cosme, Marcos Vinícius Nunes, Nárcia Mariana Fonseca Oliveira, Aldeídia Pereira de Oliveira, Elisângela Cláudia Alves de |
author_role |
author |
author2 |
Nunes, Nárcia Mariana Fonseca Oliveira, Aldeídia Pereira de Oliveira, Elisângela Cláudia Alves de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cosme, Marcos Vinícius Nunes, Nárcia Mariana Fonseca Oliveira, Aldeídia Pereira de Oliveira, Elisângela Cláudia Alves de |
dc.subject.por.fl_str_mv |
Allium cepa; cytotoxicity; genotoxicity; mutagenesis. Allium cepa; cytotoxicity; genotoxicity; mutagenesis. |
topic |
Allium cepa; cytotoxicity; genotoxicity; mutagenesis. Allium cepa; cytotoxicity; genotoxicity; mutagenesis. |
description |
The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665 10.4025/actascibiolsci.v44i1.62665 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665 |
identifier_str_mv |
10.4025/actascibiolsci.v44i1.62665 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/62665/751375154969 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 44 (2022): Publicação contínua; e62665 Acta Scientiarum. Biological Sciences; v. 44 (2022): Publicação contínua; e62665 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317391428026368 |