Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539

Detalhes bibliográficos
Autor(a) principal: Fermino, Fabiana Schumacher
Data de Publicação: 2008
Outros Autores: Bicudo, Denise de Campos, Mercante, Cacilda Thais Janson
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1539
Resumo: This study evaluated the nutrient-diffusing substrate method in laboratory under controlled temperature (20oC), for further application in in situ bioassay enrichment experiments. The experimental unit comprised a 300mL polystyrene pot filled up with 2% agar solution with isolated or combined addition of nitrogen (0.75 M NaNO3) and phosphorus (0.05 M KH2PO4). The topside opening of the pot was covered with plankton net cloth used for periphyton growth. Nutrients daily release rates were determined during 33 days. Diffusion coefficients obtained by regression analysis were similar in all treatments (0.05day-1). Results indicated that nutrient diffusion was not influenced by isolated or combined addition, thus allowing comparison between the treatments. In addition, after 4 days N:P diffusing ratio was kept close to the previously established condition. The proposed method has a potential use for nutrient limitation assessments in tropical aquatic ecosystems
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spelling Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539Substrato difusor de nutrientes (SDN): avaliação do método em laboratório para experimentos in situ com perifíton - DOI: 10.4025/actascibiolsci.v26i3.1539enriquecimentodifusãonitrogêniofósforometodologiaperifíton2.01.00.00-0 Biologia GeralThis study evaluated the nutrient-diffusing substrate method in laboratory under controlled temperature (20oC), for further application in in situ bioassay enrichment experiments. The experimental unit comprised a 300mL polystyrene pot filled up with 2% agar solution with isolated or combined addition of nitrogen (0.75 M NaNO3) and phosphorus (0.05 M KH2PO4). The topside opening of the pot was covered with plankton net cloth used for periphyton growth. Nutrients daily release rates were determined during 33 days. Diffusion coefficients obtained by regression analysis were similar in all treatments (0.05day-1). Results indicated that nutrient diffusion was not influenced by isolated or combined addition, thus allowing comparison between the treatments. In addition, after 4 days N:P diffusing ratio was kept close to the previously established condition. The proposed method has a potential use for nutrient limitation assessments in tropical aquatic ecosystemsO estudo avaliou a metodologia do substrato difusor de nutrientes em condições controladas de laboratório (20oC), visando posterior aplicação em ensaios de enriquecimento in situ. A unidade experimental constou de recipiente (copo de poliestireno) preenchido com solução agar 2% e com adição isolada ou combinada de nitrogênio (0,75 M NaNO3) e fósforo (0,05 M KH2PO4). A abertura do recipiente foi revestida com malha de plâncton (20 µm) como substrato para o perifíton. As concentrações diárias de difusão dos sais foram determinadas durante 33 dias. Os coeficientes gerados mediante regressão linear foram iguais nos diferentes tratamentos (0,05dia-1). Conclui-se que as difusões dos sais não foram influenciadas pela adição combinada ou isolada de nutrientes permitindo a comparação entre os tratamentos. A partir do 4º dia, as razões N:P de difusão foram mantidas próximas da pré-estabelecida. A metodologia proposta tem uso potencial na avaliação da limitação por nutrientes em sistemas aquáticos tropicaisUniversidade Estadual De Maringá2008-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/153910.4025/actascibiolsci.v26i3.1539Acta Scientiarum. Biological Sciences; Vol 26 No 3 (2004); 273-280Acta Scientiarum. Biological Sciences; v. 26 n. 3 (2004); 273-2801807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1539/960Fermino, Fabiana SchumacherBicudo, Denise de CamposMercante, Cacilda Thais Jansoninfo:eu-repo/semantics/openAccess2022-11-23T17:33:12Zoai:periodicos.uem.br/ojs:article/1539Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:12Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
Substrato difusor de nutrientes (SDN): avaliação do método em laboratório para experimentos in situ com perifíton - DOI: 10.4025/actascibiolsci.v26i3.1539
title Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
spellingShingle Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
Fermino, Fabiana Schumacher
enriquecimento
difusão
nitrogênio
fósforo
metodologia
perifíton
2.01.00.00-0 Biologia Geral
title_short Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
title_full Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
title_fullStr Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
title_full_unstemmed Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
title_sort Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539
author Fermino, Fabiana Schumacher
author_facet Fermino, Fabiana Schumacher
Bicudo, Denise de Campos
Mercante, Cacilda Thais Janson
author_role author
author2 Bicudo, Denise de Campos
Mercante, Cacilda Thais Janson
author2_role author
author
dc.contributor.author.fl_str_mv Fermino, Fabiana Schumacher
Bicudo, Denise de Campos
Mercante, Cacilda Thais Janson
dc.subject.por.fl_str_mv enriquecimento
difusão
nitrogênio
fósforo
metodologia
perifíton
2.01.00.00-0 Biologia Geral
topic enriquecimento
difusão
nitrogênio
fósforo
metodologia
perifíton
2.01.00.00-0 Biologia Geral
description This study evaluated the nutrient-diffusing substrate method in laboratory under controlled temperature (20oC), for further application in in situ bioassay enrichment experiments. The experimental unit comprised a 300mL polystyrene pot filled up with 2% agar solution with isolated or combined addition of nitrogen (0.75 M NaNO3) and phosphorus (0.05 M KH2PO4). The topside opening of the pot was covered with plankton net cloth used for periphyton growth. Nutrients daily release rates were determined during 33 days. Diffusion coefficients obtained by regression analysis were similar in all treatments (0.05day-1). Results indicated that nutrient diffusion was not influenced by isolated or combined addition, thus allowing comparison between the treatments. In addition, after 4 days N:P diffusing ratio was kept close to the previously established condition. The proposed method has a potential use for nutrient limitation assessments in tropical aquatic ecosystems
publishDate 2008
dc.date.none.fl_str_mv 2008-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1539
10.4025/actascibiolsci.v26i3.1539
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1539
identifier_str_mv 10.4025/actascibiolsci.v26i3.1539
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1539/960
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 26 No 3 (2004); 273-280
Acta Scientiarum. Biological Sciences; v. 26 n. 3 (2004); 273-280
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
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instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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