Determination of the biodegradability of chitosan utilizing the most probable number technique
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52965 |
Resumo: | The present work aimed to evaluate the degradability of the chitosan polymer by soil microorganisms. This evaluation was accomplished using the Most Probable Number (MPN) method by plating in drops so that soil microorganisms capable of degrading the polymeric material could be quantified. Soil samples diluted in three specific culture media for each type of microorganism were plated – bacteria, fungi and actinobacteria – and they were maintained at 28°C for seven days to determine the growth rate of fungi and actinobacteria, and for 48 hours for the development of bacteria. Significant differences in the MPN of actinobacteria relative to the other groups analyzed were observed. Thus, the method used was effective for determining the degradability of the chitosan biopolymer when observing the development of microorganisms subjected to the replacement of the carbon source by the addition of 2% w v-1 of the chitosan biopolymer to the culture medium. The formation of clear regions around the microbial colonies was a strong indicator of biodegradation. |
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Determination of the biodegradability of chitosan utilizing the most probable number techniqueDETERMINACIÓN DE LA BIODEGRADABILIDAD DE CHITOSAN UTILIZANDO LA TÉCNICA DE NÚMERO MÁS PROBABLEDetermination of the biodegradability of chitosan utilizing the most probable number techniqueActinobacteria; carbon source; microorganism.Actinobacteria; carbon source; microorganism.The present work aimed to evaluate the degradability of the chitosan polymer by soil microorganisms. This evaluation was accomplished using the Most Probable Number (MPN) method by plating in drops so that soil microorganisms capable of degrading the polymeric material could be quantified. Soil samples diluted in three specific culture media for each type of microorganism were plated – bacteria, fungi and actinobacteria – and they were maintained at 28°C for seven days to determine the growth rate of fungi and actinobacteria, and for 48 hours for the development of bacteria. Significant differences in the MPN of actinobacteria relative to the other groups analyzed were observed. Thus, the method used was effective for determining the degradability of the chitosan biopolymer when observing the development of microorganisms subjected to the replacement of the carbon source by the addition of 2% w v-1 of the chitosan biopolymer to the culture medium. The formation of clear regions around the microbial colonies was a strong indicator of biodegradation.El presente trabajo tuvo como objetivo evaluar la degradabilidad del polímero de quitosano por los microorganismos del suelo. Esta evaluación se realizó utilizando el método de Número más probable (NMP), enchapado en gotas para cuantificar los microorganismos del suelo capaces de degradar el material polimérico. Las muestras de suelo diluidas en tres medios de cultivo específicos para cada tipo de microorganismo (bacterias, hongos y actinobacterias) se colocaron en placas y se mantuvieron a 28 ° C durante siete días para determinar la tasa de crecimiento de hongos y actinobacterias y durante 48 h para el desarrollo de bacterias Se observaron diferencias significativas en la MPN de las actinobacterias en relación con los otros grupos analizados. Por lo tanto, el método utilizado fue efectivo para determinar la degradabilidad del biopolímero de quitosano al observar el desarrollo de microorganismos sujetos a la sustitución de la fuente de carbono al agregar 2% p / v del biopolímero de quitosano al medio de cultivo. La formación de regiones claras alrededor de colonias microbianas fue un fuerte indicador de biodegradación.The present work aimed to evaluate the degradability of the chitosan polymer by soil microorganisms. This evaluation was accomplished using the Most Probable Number (MPN) method by plating in drops so that soil microorganisms capable of degrading the polymeric material could be quantified. Soil samples diluted in three specific culture media for each type of microorganism were plated – bacteria, fungi and actinobacteria – and they were maintained at 28°C for seven days to determine the growth rate of fungi and actinobacteria, and for 48 hours for the development of bacteria. Significant differences in the MPN of actinobacteria relative to the other groups analyzed were observed. Thus, the method used was effective for determining the degradability of the chitosan biopolymer when observing the development of microorganisms subjected to the replacement of the carbon source by the addition of 2% w v-1 of the chitosan biopolymer to the culture medium. The formation of clear regions around the microbial colonies was a strong indicator of biodegradation.Universidade Estadual De Maringá2020-08-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5296510.4025/actascibiolsci.v42i1.52965Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e52965Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e529651807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52965/751375150627Copyright (c) 2020 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Wender Cardoso Souza, Ilva de Fátima Benassi, Vivian Machado Roa, Juan Pedro BretasGraziotti, Paulo Henrique Nelson, David Lee Reis, Arlete Barbosa dos2020-11-16T16:25:43Zoai:periodicos.uem.br/ojs:article/52965Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2020-11-16T16:25:43Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Determination of the biodegradability of chitosan utilizing the most probable number technique DETERMINACIÓN DE LA BIODEGRADABILIDAD DE CHITOSAN UTILIZANDO LA TÉCNICA DE NÚMERO MÁS PROBABLE Determination of the biodegradability of chitosan utilizing the most probable number technique |
title |
Determination of the biodegradability of chitosan utilizing the most probable number technique |
spellingShingle |
Determination of the biodegradability of chitosan utilizing the most probable number technique Silva, Wender Cardoso Actinobacteria; carbon source; microorganism. Actinobacteria; carbon source; microorganism. |
title_short |
Determination of the biodegradability of chitosan utilizing the most probable number technique |
title_full |
Determination of the biodegradability of chitosan utilizing the most probable number technique |
title_fullStr |
Determination of the biodegradability of chitosan utilizing the most probable number technique |
title_full_unstemmed |
Determination of the biodegradability of chitosan utilizing the most probable number technique |
title_sort |
Determination of the biodegradability of chitosan utilizing the most probable number technique |
author |
Silva, Wender Cardoso |
author_facet |
Silva, Wender Cardoso Souza, Ilva de Fátima Benassi, Vivian Machado Roa, Juan Pedro Bretas Graziotti, Paulo Henrique Nelson, David Lee Reis, Arlete Barbosa dos |
author_role |
author |
author2 |
Souza, Ilva de Fátima Benassi, Vivian Machado Roa, Juan Pedro Bretas Graziotti, Paulo Henrique Nelson, David Lee Reis, Arlete Barbosa dos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Wender Cardoso Souza, Ilva de Fátima Benassi, Vivian Machado Roa, Juan Pedro Bretas Graziotti, Paulo Henrique Nelson, David Lee Reis, Arlete Barbosa dos |
dc.subject.por.fl_str_mv |
Actinobacteria; carbon source; microorganism. Actinobacteria; carbon source; microorganism. |
topic |
Actinobacteria; carbon source; microorganism. Actinobacteria; carbon source; microorganism. |
description |
The present work aimed to evaluate the degradability of the chitosan polymer by soil microorganisms. This evaluation was accomplished using the Most Probable Number (MPN) method by plating in drops so that soil microorganisms capable of degrading the polymeric material could be quantified. Soil samples diluted in three specific culture media for each type of microorganism were plated – bacteria, fungi and actinobacteria – and they were maintained at 28°C for seven days to determine the growth rate of fungi and actinobacteria, and for 48 hours for the development of bacteria. Significant differences in the MPN of actinobacteria relative to the other groups analyzed were observed. Thus, the method used was effective for determining the degradability of the chitosan biopolymer when observing the development of microorganisms subjected to the replacement of the carbon source by the addition of 2% w v-1 of the chitosan biopolymer to the culture medium. The formation of clear regions around the microbial colonies was a strong indicator of biodegradation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52965 10.4025/actascibiolsci.v42i1.52965 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52965 |
identifier_str_mv |
10.4025/actascibiolsci.v42i1.52965 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52965/751375150627 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e52965 Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e52965 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317397859991552 |