Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1355 |
Resumo: | The production of alkaloids from Cereus peruvianus callus cultured in medium supplemented with different tyrosine concentrations, different 2,4-D/kinetin levels, and with NaCl was investigated using long-term callus culture. α- and β-Esterase isozyme patterns were also analyzed to investigate the differential gene expression in callus subcultured with the different tyrosine concentrations. The greatest amounts of alkaloids were obtained from callus maintained in medium containing 200 mg/L of tyrosine. Tyrosine induced a differential gene expression for the synthesis of specific α- and β -esterase isozymes. Two α-carboxylesterases (EC 3.1.1.1) and one α/β-arylesterase (EC 3.1.1.2) were induced in callus, one α/β-acetylesterase (EC 3.1.1.6) and two α-carboxylesterase were detected as more intensely stained while two α/β-carboxylesterases were absent or detected as more weakly stained bands after the addition of tyrosine as precursor |
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Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355Efeito de diferentes componentes no meio de cultura na produção de alcalóides em tecidos de calos de Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355alcalóidescactoscultura de tecidos de caloscactos colunarmandacarutirosina2.01.00.00-0 Biologia GeralThe production of alkaloids from Cereus peruvianus callus cultured in medium supplemented with different tyrosine concentrations, different 2,4-D/kinetin levels, and with NaCl was investigated using long-term callus culture. α- and β-Esterase isozyme patterns were also analyzed to investigate the differential gene expression in callus subcultured with the different tyrosine concentrations. The greatest amounts of alkaloids were obtained from callus maintained in medium containing 200 mg/L of tyrosine. Tyrosine induced a differential gene expression for the synthesis of specific α- and β -esterase isozymes. Two α-carboxylesterases (EC 3.1.1.1) and one α/β-arylesterase (EC 3.1.1.2) were induced in callus, one α/β-acetylesterase (EC 3.1.1.6) and two α-carboxylesterase were detected as more intensely stained while two α/β-carboxylesterases were absent or detected as more weakly stained bands after the addition of tyrosine as precursorTecidos de calos em cultura prolongada de Cereus peruvianus foram utilizados para investigar a produção de alcalóides pelos calos quando mantidos em meio de cultura suplementados com diferentes concentrações de tirosina, com diferentes níveis de 2,4-D/cinetina, e contendo NaCl. Foi analisado também, o padrão de isozimas α- e β-esterases, para investigar a expressão diferencial de genes nos calos subcultivados com as diferentes concentrações de tirosina. A maior quantidade de alcalóides foi obtida a partir de calos mantidos em meio contendo 200 mg/L de tirosina. A adição de tirosina no meio induziu uma expressão gênica diferencial para a síntese de isozimas α- e β-esterases. Duas α-carboxilesterases (EC 3.1.1.1) e uma α/β-arilesterase (EC 3.1.1.2) foram induzidas nos calos, uma α/β-acetilesterase (EC 3.1.1.6) e uma α-carboxilesterase foram detectadas como bandas mais intensamente coradas, enquanto duas α/β-carboxilesterases não foram detectadas ou foram fracamente coradas após a adição de tirosina como precursor no meio de culturaUniversidade Estadual De Maringá2008-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/135510.4025/actascibiolsci.v27i1.1355Acta Scientiarum. Biological Sciences; Vol 27 No 1 (2005); 37-41Acta Scientiarum. Biological Sciences; v. 27 n. 1 (2005); 37-411807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1355/772Rocha, Luciano Kleber daOliveira, Arildo José Braz deMangolin, Claudete AparecidaMachado, Maria de Fátima Pires da Silvainfo:eu-repo/semantics/openAccess2022-11-23T17:33:08Zoai:periodicos.uem.br/ojs:article/1355Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:08Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 Efeito de diferentes componentes no meio de cultura na produção de alcalóides em tecidos de calos de Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 |
title |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 |
spellingShingle |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 Rocha, Luciano Kleber da alcalóides cactos cultura de tecidos de calos cactos colunar mandacaru tirosina 2.01.00.00-0 Biologia Geral |
title_short |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 |
title_full |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 |
title_fullStr |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 |
title_full_unstemmed |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 |
title_sort |
Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355 |
author |
Rocha, Luciano Kleber da |
author_facet |
Rocha, Luciano Kleber da Oliveira, Arildo José Braz de Mangolin, Claudete Aparecida Machado, Maria de Fátima Pires da Silva |
author_role |
author |
author2 |
Oliveira, Arildo José Braz de Mangolin, Claudete Aparecida Machado, Maria de Fátima Pires da Silva |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Rocha, Luciano Kleber da Oliveira, Arildo José Braz de Mangolin, Claudete Aparecida Machado, Maria de Fátima Pires da Silva |
dc.subject.por.fl_str_mv |
alcalóides cactos cultura de tecidos de calos cactos colunar mandacaru tirosina 2.01.00.00-0 Biologia Geral |
topic |
alcalóides cactos cultura de tecidos de calos cactos colunar mandacaru tirosina 2.01.00.00-0 Biologia Geral |
description |
The production of alkaloids from Cereus peruvianus callus cultured in medium supplemented with different tyrosine concentrations, different 2,4-D/kinetin levels, and with NaCl was investigated using long-term callus culture. α- and β-Esterase isozyme patterns were also analyzed to investigate the differential gene expression in callus subcultured with the different tyrosine concentrations. The greatest amounts of alkaloids were obtained from callus maintained in medium containing 200 mg/L of tyrosine. Tyrosine induced a differential gene expression for the synthesis of specific α- and β -esterase isozymes. Two α-carboxylesterases (EC 3.1.1.1) and one α/β-arylesterase (EC 3.1.1.2) were induced in callus, one α/β-acetylesterase (EC 3.1.1.6) and two α-carboxylesterase were detected as more intensely stained while two α/β-carboxylesterases were absent or detected as more weakly stained bands after the addition of tyrosine as precursor |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1355 10.4025/actascibiolsci.v27i1.1355 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1355 |
identifier_str_mv |
10.4025/actascibiolsci.v27i1.1355 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1355/772 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 27 No 1 (2005); 37-41 Acta Scientiarum. Biological Sciences; v. 27 n. 1 (2005); 37-41 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317392081289216 |