Production and characterization of a putative T-cell marker in hybrid surubim catfish Pseudoplatystoma corruscans (Agassiz) x Pseudoplatystoma fasciatum (Linnaeus) - DOI: 10.4025/actascibiolsci.v26i2.1636

• Autor(a) principal: Beelen, Roger
• Data de Publicação: 2008
• Outros Autores: Pavanelli, Gilberto Cezar, Boyd, Bobbie, Garavello, Julio Cesar, Beelen, Patricia Mendes Guimarães, Ainsworth, Avery Jerald
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Acta Scientiarum Biological Sciences
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1636
• topic: anticorpo monoclonal; timócitos; linfócitos T; Pseudoplatystoma corruscans; Pseudoplatystoma fasciatum; 2.01.00.00-0 Biologia Geral

A monoclonal antibody against hybrid surubim thymocytes and circulating T-cells (PST 33) was produced and characterized. The mAb was obtained by immunizing mice with isolated membrane molecules of hybrid surubim thymocytes, and whole thymocytes. Flow cytometric analysis showed that mAb PST 33 reacted with 87.5% of the thymocytes, 34.1% of circulating, 19.1% of pronephros, and 36.6% of spleen lymphoid cells. Immunohistochemistry demonstrated that PST 33-immunoreactive cells are mostly present in the medullar region of the thymus, and in the vicinity of arterioles spread throughout the parenchyma of the spleen. PST 33 appeared as a suitable marker for immunohistochemistry, and should prove useful in studying the ontogeny of surubim immune system. Immunogold labeling revealed that PST 33+ cells were lymphoid-like cells. Morphologically they appeared as small lymphocytes with a high nucleus/cytoplasm ratio. Western blotting of non-reduced membrane lysates showed that PST 33 reacted with a 215 kDa molecule