Identification of the mcyA gene in natural blooms of Radiocystis fernandoi from a tributary of the Rosana reservoir, Brazil - doi: 10.4025/actascibiolsci.v33i3.6802

• Autor(a) principal: Fonseca, Iraúza Arroteia
• Data de Publicação: 2011
• Outros Autores: Maniglia, Thiago Cintra, Rodrigues, Liliana, Prioli, Alberto José, Prioli, Sônia Maria Alves Pinto
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Acta Scientiarum Biological Sciences
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6802
• topic: Cyanobacteria; toxins; PCR; Radiocystis fernandoi; mcyA; Cianobactéria; toxina; PCR; Radiocystis fernandoi; mcyA; Genética

Cyanobacterias are known as toxin producers. Within the same species, toxic and non-toxic varieties can be found and it is impossible to differentiate them only by morphology. The most important toxin produced by cyanobacteria is microcystin. This protein is synthesized by a cluster of genes known as mcy. The detection of these genes by PCR allows the differentiation of the producing toxin strain from the non-producing toxin strain. Thus, the goal of this work was to investigate the occurrence of toxigenic blooms of cyanobacteria in the Corvo River through PCR amplification of mcyA gene. For this, two samples of blooms of cyanobacteria were collected in Corvo River. Both samples were dominated by Radiocystis fernandoi and presented positive results for the presence of the mcyA gene, which may confirm the potential toxigenicity for that species. These results are an alert about water quality in the Corvo River. Here we demonstrate that amplification of the mcyA gene by PCR is a fast, cheap and efficient method for the detection of toxin- producing cyanobacteria.