Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548

Detalhes bibliográficos
Autor(a) principal: Kamada, Takeshi
Data de Publicação: 2009
Outros Autores: Picoli, Edgard Augusto de Toledo, Alfenas, Acelino Couto, Cruz, Cosme Damião, Vieira, Roberto Fontes, Otoni, Wagner Campos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/548
Resumo: Pfaffia glomerata is a native species from South America, occurring naturally in some states of Brazil. The ever growing interest by the species, mainly due to its important therapeutic properties, has threatened the natural populations, as exploitation has occurred in a rather predatory way. The present study aimed to compare the genetic diversity of four natural populations originated from of the area of the Paraná River basin (Paraná and Mato Grosso States). The individuals were propagated in vitro and characterized by means of RAPD markers. A total of 267 polymorphic marks were obtained from 67 primers. The genetic distance estimates were obtained based on the complement arithmetic of Nei and Li’s similarity coefficient, and the genotypes were grouped according to the UPGMA method. Three populations from the margin of the Paraná River and at Mineiro Island presented low diversity levels among them, though possessing the same propagule dispersal flow. The population located in the proximities of the margin of the Ivaí river (tributary of the river Paraná) presented greater diversity values within and among the populations, suggesting that its preservation should be a priority and that the origin of the genetic variability may be related to the dispersion flow of the vegetative propagules.
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spelling Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548Diversidade genética de populações naturais de Pfaffia glomerata (Spreng.) Pedersen estimada por marcadores RAPD - DOI: 10.4025/actasciagron.v31i3.548brazilian-ginsenggermplasm conservationplant breeding‘ginseng brasileiro’conservação de germoplasmamelhoramento vegetal5.01.03.05-9Pfaffia glomerata is a native species from South America, occurring naturally in some states of Brazil. The ever growing interest by the species, mainly due to its important therapeutic properties, has threatened the natural populations, as exploitation has occurred in a rather predatory way. The present study aimed to compare the genetic diversity of four natural populations originated from of the area of the Paraná River basin (Paraná and Mato Grosso States). The individuals were propagated in vitro and characterized by means of RAPD markers. A total of 267 polymorphic marks were obtained from 67 primers. The genetic distance estimates were obtained based on the complement arithmetic of Nei and Li’s similarity coefficient, and the genotypes were grouped according to the UPGMA method. Three populations from the margin of the Paraná River and at Mineiro Island presented low diversity levels among them, though possessing the same propagule dispersal flow. The population located in the proximities of the margin of the Ivaí river (tributary of the river Paraná) presented greater diversity values within and among the populations, suggesting that its preservation should be a priority and that the origin of the genetic variability may be related to the dispersion flow of the vegetative propagules.Pfaffia glomerata é uma espécie nativa da América do Sul e de ocorrência natural em alguns Estados do Brasil. Porém, as populações naturais de fáfia têm sido ameaçadas em decorrência do intenso extrativismo, em virtude de suas propriedades terapêuticas de interesse. No presente estudo, objetivou-se estimar a diversidade genética de quatro populações naturais oriundas da bacia do Rio Paraná. Os indivíduos foram multiplicados in vitro e caracterizados de acordo com o padrão eletroforético de marcadores RAPD, utilizando-se 268 marcas polimórficas obtidas a partir de 67 primers. As estimativas de distância genética foram obtidas pelo complemento aritmético do coeficiente de Nei e Li, e os genótipos agrupados de acordo com o método UPGMA. Três populações situadas próximas às margens do rio Paraná e na ilha do Mineiro apresentaram baixos índices de diversidade entre elas, e estas possuem o mesmo fluxo de dispersão dos propágulos. A população situada nas proximidades da margem do rio Ivaí (afluente do rio Paraná) apresentou maiores valores de diversidade dentro da população e entre as populações, indicando que sua preservação é prioritária e a origem da variabilidade pode estar relacionada ao fluxo de dispersão de propágulos.Universidade Estadual de Maringá2009-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/54810.4025/actasciagron.v31i3.548Acta Scientiarum. Agronomy; Vol 31 No 3 (2009); 403-409Acta Scientiarum. Agronomy; v. 31 n. 3 (2009); 403-4091807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/548/548Kamada, TakeshiPicoli, Edgard Augusto de ToledoAlfenas, Acelino CoutoCruz, Cosme DamiãoVieira, Roberto FontesOtoni, Wagner Camposinfo:eu-repo/semantics/openAccess2022-11-23T18:37:08Zoai:periodicos.uem.br/ojs:article/548Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:37:08Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
Diversidade genética de populações naturais de Pfaffia glomerata (Spreng.) Pedersen estimada por marcadores RAPD - DOI: 10.4025/actasciagron.v31i3.548
title Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
spellingShingle Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
Kamada, Takeshi
brazilian-ginseng
germplasm conservation
plant breeding
‘ginseng brasileiro’
conservação de germoplasma
melhoramento vegetal
5.01.03.05-9
title_short Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
title_full Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
title_fullStr Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
title_full_unstemmed Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
title_sort Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548
author Kamada, Takeshi
author_facet Kamada, Takeshi
Picoli, Edgard Augusto de Toledo
Alfenas, Acelino Couto
Cruz, Cosme Damião
Vieira, Roberto Fontes
Otoni, Wagner Campos
author_role author
author2 Picoli, Edgard Augusto de Toledo
Alfenas, Acelino Couto
Cruz, Cosme Damião
Vieira, Roberto Fontes
Otoni, Wagner Campos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Kamada, Takeshi
Picoli, Edgard Augusto de Toledo
Alfenas, Acelino Couto
Cruz, Cosme Damião
Vieira, Roberto Fontes
Otoni, Wagner Campos
dc.subject.por.fl_str_mv brazilian-ginseng
germplasm conservation
plant breeding
‘ginseng brasileiro’
conservação de germoplasma
melhoramento vegetal
5.01.03.05-9
topic brazilian-ginseng
germplasm conservation
plant breeding
‘ginseng brasileiro’
conservação de germoplasma
melhoramento vegetal
5.01.03.05-9
description Pfaffia glomerata is a native species from South America, occurring naturally in some states of Brazil. The ever growing interest by the species, mainly due to its important therapeutic properties, has threatened the natural populations, as exploitation has occurred in a rather predatory way. The present study aimed to compare the genetic diversity of four natural populations originated from of the area of the Paraná River basin (Paraná and Mato Grosso States). The individuals were propagated in vitro and characterized by means of RAPD markers. A total of 267 polymorphic marks were obtained from 67 primers. The genetic distance estimates were obtained based on the complement arithmetic of Nei and Li’s similarity coefficient, and the genotypes were grouped according to the UPGMA method. Three populations from the margin of the Paraná River and at Mineiro Island presented low diversity levels among them, though possessing the same propagule dispersal flow. The population located in the proximities of the margin of the Ivaí river (tributary of the river Paraná) presented greater diversity values within and among the populations, suggesting that its preservation should be a priority and that the origin of the genetic variability may be related to the dispersion flow of the vegetative propagules.
publishDate 2009
dc.date.none.fl_str_mv 2009-06-23
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dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/548
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url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/548
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dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/548/548
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 31 No 3 (2009); 403-409
Acta Scientiarum. Agronomy; v. 31 n. 3 (2009); 403-409
1807-8621
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