Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5962 |
Resumo: | The present work aims to identify 11 isolates of Guignardia citricarpa, the causal agent of the citrus black spot (CBS), obtained from affected fruit in different geographical regions, through PCR and morphophysiological characterization of propagative structures, sporing and mycelial growth in different means of culture, temperatures and photoperiods, under laboratory conditions. Through the PCR test, all isolates were identified as being the G. citricarpa pathogen. The characterized isolates were subjected to evaluations at temperatures of 20, 25 and 30ºC, in continuous light, continuous darkness, and alternating 12 hours light and 12 hours darkness, during 24 days. The study used oat-agar (OA), potato-dextrose-agar (PDA) and carrot-dextrose-agar (CDA) as means of culture. The results showed an interaction between the different means of culture, temperatures and photoperiods. The mean of culture that best stimulated mycelial growth was CDA at 25ºC under the 12-hour photoperiod. The greatest spore (conidia) production was verified in PDA at 20ºC in the 12-hour photoperiod. There was no sporing of any isolate in the CDA mean of culture. Under the 30ºC temperature, the study verified only the production of hyphae and picnids in most isolates, in all means of culture and photoperiods. |
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Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962Caracterização morfofisiológica e identificação molecular de isolados de Guignardia citricarpa, agente patogênico da mancha preta dos citros - DOI: 10.4025/actasciagron.v30i5.5962G. citricarpaPCRsporingmycelial growthtemperaturemean of culturephotoperiodG. citricarpaPCResporulaçãocrescimento micelialtemperaturameio de culturafotoperíodo.The present work aims to identify 11 isolates of Guignardia citricarpa, the causal agent of the citrus black spot (CBS), obtained from affected fruit in different geographical regions, through PCR and morphophysiological characterization of propagative structures, sporing and mycelial growth in different means of culture, temperatures and photoperiods, under laboratory conditions. Through the PCR test, all isolates were identified as being the G. citricarpa pathogen. The characterized isolates were subjected to evaluations at temperatures of 20, 25 and 30ºC, in continuous light, continuous darkness, and alternating 12 hours light and 12 hours darkness, during 24 days. The study used oat-agar (OA), potato-dextrose-agar (PDA) and carrot-dextrose-agar (CDA) as means of culture. The results showed an interaction between the different means of culture, temperatures and photoperiods. The mean of culture that best stimulated mycelial growth was CDA at 25ºC under the 12-hour photoperiod. The greatest spore (conidia) production was verified in PDA at 20ºC in the 12-hour photoperiod. There was no sporing of any isolate in the CDA mean of culture. Under the 30ºC temperature, the study verified only the production of hyphae and picnids in most isolates, in all means of culture and photoperiods.O presente trabalho teve como objetivo identificar 11 isolados de Guignardia citricarpa, agente causal da mancha preta dos citros (MPC), obtidos de frutas cítricas sintomáticas de diferentes regiões geográficas, por meio da PCR e caracterização morfofisiológica das estruturas propagativas, esporulação e crescimento micelial em diferentes meios de cultura, temperaturas e regimes de luz, nas condições de laboratório. Pelo teste de PCR, todos os isolados foram identificados como o patógeno G. citricarpa. Os isolados caracterizados foram submetidos às temperaturas de 20, 25 e 30ºC, em regime de luz contínua, escuro contínuo e fotoperíodos de 12 horas, durante 24 dias. Utilizaram-se os meios de cultura aveia-ágar (AA), batata-dextrose-ágar (BDA) e cenoura-dextrose-ágar (CDA). Os resultados mostraram que ocorreu interação entre os diferentes meios de cultura, temperaturas e fotoperíodos. O meio de cultura que melhor estimulou o crescimento micelial foi o CDA a 25ºC sob o fotoperíodo de 12h. A maior produção de esporos (conídios) foi verificada no meio BDA a 20ºC, no fotoperíodo de 12 horas. No meio CDA, não ocorreu esporulação de nenhum isolado. Sob a temperatura de 30ºC, foi verificada apenas a produção de hifas e picnídios para a maioria dos isolados, em todos os meios de cultura e fotoperíodo testados.Universidade Estadual de Maringá2008-12-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/596210.4025/actasciagron.v30i5.5962Acta Scientiarum. Agronomy; Vol 30 No 5 (2008): Special suplemment; 625-630Acta Scientiarum. Agronomy; v. 30 n. 5 (2008): Suplemento Especial; 625-6301807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5962/5962Caixeta, Marilda PereiraCorazza, Maria JúliaOliveira, Ricardo Ribeiro deZanutto, Carlos AlexandreNunes, William Mário de CarvalhoVida, João Batistainfo:eu-repo/semantics/openAccess2022-11-23T18:38:14Zoai:periodicos.uem.br/ojs:article/5962Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:14Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 Caracterização morfofisiológica e identificação molecular de isolados de Guignardia citricarpa, agente patogênico da mancha preta dos citros - DOI: 10.4025/actasciagron.v30i5.5962 |
title |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 |
spellingShingle |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 Caixeta, Marilda Pereira G. citricarpa PCR sporing mycelial growth temperature mean of culture photoperiod G. citricarpa PCR esporulação crescimento micelial temperatura meio de cultura fotoperíodo. |
title_short |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 |
title_full |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 |
title_fullStr |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 |
title_full_unstemmed |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 |
title_sort |
Morphophysiological characterization and molecular identification of isolates of Guignardia citricarpa, a pathogenic agent of the citrus black spot - DOI: 10.4025/actasciagron.v30i5.5962 |
author |
Caixeta, Marilda Pereira |
author_facet |
Caixeta, Marilda Pereira Corazza, Maria Júlia Oliveira, Ricardo Ribeiro de Zanutto, Carlos Alexandre Nunes, William Mário de Carvalho Vida, João Batista |
author_role |
author |
author2 |
Corazza, Maria Júlia Oliveira, Ricardo Ribeiro de Zanutto, Carlos Alexandre Nunes, William Mário de Carvalho Vida, João Batista |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Caixeta, Marilda Pereira Corazza, Maria Júlia Oliveira, Ricardo Ribeiro de Zanutto, Carlos Alexandre Nunes, William Mário de Carvalho Vida, João Batista |
dc.subject.por.fl_str_mv |
G. citricarpa PCR sporing mycelial growth temperature mean of culture photoperiod G. citricarpa PCR esporulação crescimento micelial temperatura meio de cultura fotoperíodo. |
topic |
G. citricarpa PCR sporing mycelial growth temperature mean of culture photoperiod G. citricarpa PCR esporulação crescimento micelial temperatura meio de cultura fotoperíodo. |
description |
The present work aims to identify 11 isolates of Guignardia citricarpa, the causal agent of the citrus black spot (CBS), obtained from affected fruit in different geographical regions, through PCR and morphophysiological characterization of propagative structures, sporing and mycelial growth in different means of culture, temperatures and photoperiods, under laboratory conditions. Through the PCR test, all isolates were identified as being the G. citricarpa pathogen. The characterized isolates were subjected to evaluations at temperatures of 20, 25 and 30ºC, in continuous light, continuous darkness, and alternating 12 hours light and 12 hours darkness, during 24 days. The study used oat-agar (OA), potato-dextrose-agar (PDA) and carrot-dextrose-agar (CDA) as means of culture. The results showed an interaction between the different means of culture, temperatures and photoperiods. The mean of culture that best stimulated mycelial growth was CDA at 25ºC under the 12-hour photoperiod. The greatest spore (conidia) production was verified in PDA at 20ºC in the 12-hour photoperiod. There was no sporing of any isolate in the CDA mean of culture. Under the 30ºC temperature, the study verified only the production of hyphae and picnids in most isolates, in all means of culture and photoperiods. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5962 10.4025/actasciagron.v30i5.5962 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5962 |
identifier_str_mv |
10.4025/actasciagron.v30i5.5962 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5962/5962 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 30 No 5 (2008): Special suplemment; 625-630 Acta Scientiarum. Agronomy; v. 30 n. 5 (2008): Suplemento Especial; 625-630 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305907160481792 |