AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577 |
Resumo: | The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair Cluster Method with Arithmetic Mean (UPGMA), principal coordinates analysis (PCoA) and Bayesian statistics were used for the clustering analysis. The three combinations of primer/enzyme revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. |
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Acta Scientiarum. Agronomy (Online) |
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AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessionsAFLP analysis of genetic diversity in determinate and indeterminate snap bean accessionsPhaseolus vulgaris L.molecular markersAmplified Fragment Length Polymorphismgene bankgenetic diversityRecursos GenéticosPhaseolus vulgaris L.molecular markersamplified fragment length polymorphismgene bankThe present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair Cluster Method with Arithmetic Mean (UPGMA), principal coordinates analysis (PCoA) and Bayesian statistics were used for the clustering analysis. The three combinations of primer/enzyme revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. These selective AFLP combinations revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. Universidade Estadual de Maringá2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2557710.4025/actasciagron.v38i1.25577Acta Scientiarum. Agronomy; Vol 38 No 1 (2016); 29-34Acta Scientiarum. Agronomy; v. 38 n. 1 (2016); 29-341807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/pdf_110http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/751375143394Andrade, Felipe Aranha deGonçalves, Leandro Simões AzeredoMiglioranza, ÉdisonRuas, Claudete de FátimaRuas, Paulo MauricioTakahashi, Lúcia Sadayo Assariinfo:eu-repo/semantics/openAccess2016-02-17T15:35:41Zoai:periodicos.uem.br/ojs:article/25577Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2016-02-17T15:35:41Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
title |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
spellingShingle |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions Andrade, Felipe Aranha de Phaseolus vulgaris L. molecular markers Amplified Fragment Length Polymorphism gene bank genetic diversity Recursos Genéticos Phaseolus vulgaris L. molecular markers amplified fragment length polymorphism gene bank |
title_short |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
title_full |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
title_fullStr |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
title_full_unstemmed |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
title_sort |
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
author |
Andrade, Felipe Aranha de |
author_facet |
Andrade, Felipe Aranha de Gonçalves, Leandro Simões Azeredo Miglioranza, Édison Ruas, Claudete de Fátima Ruas, Paulo Mauricio Takahashi, Lúcia Sadayo Assari |
author_role |
author |
author2 |
Gonçalves, Leandro Simões Azeredo Miglioranza, Édison Ruas, Claudete de Fátima Ruas, Paulo Mauricio Takahashi, Lúcia Sadayo Assari |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Andrade, Felipe Aranha de Gonçalves, Leandro Simões Azeredo Miglioranza, Édison Ruas, Claudete de Fátima Ruas, Paulo Mauricio Takahashi, Lúcia Sadayo Assari |
dc.subject.por.fl_str_mv |
Phaseolus vulgaris L. molecular markers Amplified Fragment Length Polymorphism gene bank genetic diversity Recursos Genéticos Phaseolus vulgaris L. molecular markers amplified fragment length polymorphism gene bank |
topic |
Phaseolus vulgaris L. molecular markers Amplified Fragment Length Polymorphism gene bank genetic diversity Recursos Genéticos Phaseolus vulgaris L. molecular markers amplified fragment length polymorphism gene bank |
description |
The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair Cluster Method with Arithmetic Mean (UPGMA), principal coordinates analysis (PCoA) and Bayesian statistics were used for the clustering analysis. The three combinations of primer/enzyme revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577 10.4025/actasciagron.v38i1.25577 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577 |
identifier_str_mv |
10.4025/actasciagron.v38i1.25577 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/pdf_110 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/751375143394 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 38 No 1 (2016); 29-34 Acta Scientiarum. Agronomy; v. 38 n. 1 (2016); 29-34 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
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1799305909364588544 |