Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA

Detalhes bibliográficos
Autor(a) principal: Kuramae, Eiko Eurya
Data de Publicação: 2008
Outros Autores: de Souza, Nilton Luiz
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2409
Resumo: Differentiation from formae specials and races of Fusarium oxysporum by morphological and cultural characteristics is difficult and uncertain. Random amplified polymorphic DNA (RAPD) markers and ITS1 and ITS2 (internal transcribed spacer) sequences were used to evaluate genetic variability among four different formae specials of Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) and two races (1 and 2) of F. o. lycopersici. Genetic variability up to 50% was observed among the formae specials and 7% between races 1 and 2 of F. o. lycopersici using RAPD molecular markers. Similarity more than 97.2% among formae specials and races 1 and 2 of F. o. lycopersici was observed at ITS1 and ITS2 regions, whereas the 5.8S rDNA gene was 100% homologous among isolates
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spelling Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNAVariabilidade genética entre formae speciales de Fusarium oxysporum e raças 1 e 2 de F. oxysporum f. sp. lycopersici através de RAPD e sequências de regiões ITS e rDNAFusarium oxysporumvariabilidade genéticaITS5.00.00.00-4 Ciências AgráriasDifferentiation from formae specials and races of Fusarium oxysporum by morphological and cultural characteristics is difficult and uncertain. Random amplified polymorphic DNA (RAPD) markers and ITS1 and ITS2 (internal transcribed spacer) sequences were used to evaluate genetic variability among four different formae specials of Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) and two races (1 and 2) of F. o. lycopersici. Genetic variability up to 50% was observed among the formae specials and 7% between races 1 and 2 of F. o. lycopersici using RAPD molecular markers. Similarity more than 97.2% among formae specials and races 1 and 2 of F. o. lycopersici was observed at ITS1 and ITS2 regions, whereas the 5.8S rDNA gene was 100% homologous among isolatesDiferenciação de formae speciales e raças de Fusarium oxysporum através de características morfológicas e culturais é bastante difícil e muitas vezes duvidosa. Marcadores moleculares RAPD e sequenciamento das regiões ITS1 e ITS2 (internal transcribed spacer) e o gene 5.8S rDNA foram utilizados para estimar a variabilidade genética existente entre quatro formae speciales de Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) e entre 2 raças de F. o. lycopersici. A variabilidade genética em nível de DNA genômico utilizando-se marcadores moleculares RAPD foi de até 50% entre as formae speciales e, entre as raças 1 e 2 de F. o. lycopersici foi de 7%. Houve similaridade maior que 97,2% entre as diferentes formae speciales e raças fisiológicas de F. o lycopersici na regiões correspondentes a ITS1 e ITS2, enquanto que o gene 5.8S rDNA apresentou 100% de homologia entre os isoladosUniversidade Estadual de Maringá2008-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/240910.4025/actasciagron.v24i0.2409Acta Scientiarum. Agronomy; Vol 24 (2002); 1481-1485Acta Scientiarum. Agronomy; v. 24 (2002); 1481-14851807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2409/1808Kuramae, Eiko Euryade Souza, Nilton Luizinfo:eu-repo/semantics/openAccess2022-11-23T18:37:47Zoai:periodicos.uem.br/ojs:article/2409Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:37:47Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
Variabilidade genética entre formae speciales de Fusarium oxysporum e raças 1 e 2 de F. oxysporum f. sp. lycopersici através de RAPD e sequências de regiões ITS e rDNA
title Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
spellingShingle Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
Kuramae, Eiko Eurya
Fusarium oxysporum
variabilidade genética
ITS
5.00.00.00-4 Ciências Agrárias
title_short Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
title_full Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
title_fullStr Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
title_full_unstemmed Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
title_sort Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA
author Kuramae, Eiko Eurya
author_facet Kuramae, Eiko Eurya
de Souza, Nilton Luiz
author_role author
author2 de Souza, Nilton Luiz
author2_role author
dc.contributor.author.fl_str_mv Kuramae, Eiko Eurya
de Souza, Nilton Luiz
dc.subject.por.fl_str_mv Fusarium oxysporum
variabilidade genética
ITS
5.00.00.00-4 Ciências Agrárias
topic Fusarium oxysporum
variabilidade genética
ITS
5.00.00.00-4 Ciências Agrárias
description Differentiation from formae specials and races of Fusarium oxysporum by morphological and cultural characteristics is difficult and uncertain. Random amplified polymorphic DNA (RAPD) markers and ITS1 and ITS2 (internal transcribed spacer) sequences were used to evaluate genetic variability among four different formae specials of Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) and two races (1 and 2) of F. o. lycopersici. Genetic variability up to 50% was observed among the formae specials and 7% between races 1 and 2 of F. o. lycopersici using RAPD molecular markers. Similarity more than 97.2% among formae specials and races 1 and 2 of F. o. lycopersici was observed at ITS1 and ITS2 regions, whereas the 5.8S rDNA gene was 100% homologous among isolates
publishDate 2008
dc.date.none.fl_str_mv 2008-04-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2409
10.4025/actasciagron.v24i0.2409
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2409
identifier_str_mv 10.4025/actasciagron.v24i0.2409
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2409/1808
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 24 (2002); 1481-1485
Acta Scientiarum. Agronomy; v. 24 (2002); 1481-1485
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Agronomy (Online)
collection Acta Scientiarum. Agronomy (Online)
repository.name.fl_str_mv Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br
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