Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967

Detalhes bibliográficos
Autor(a) principal: Nunes, William Mário de Carvalho
Data de Publicação: 2008
Outros Autores: Zanutto, Carlos Alexandre, Corazza-Nunes, Maria Júlia, Molina, Rúbia de Oliveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/967
Resumo: Countless diseases affect the citriculture, as the citrus variegated chlorosis (CVC) which is caused by the bacteria Xylella fastidiosa. The aim of this work was to determine the space distribution of the disease inside commercial orchards in the Northwest of Paraná, using molecular methods. Symptomatic orchards were selected for CVC with the varieties 'Pêra', 'Valência' and 'Folha Murcha' (Citrus sinensis Osbeck). For each variety, 4 positive reference-plant for CVC (presence of CVC symptoms or molecular analysis by PCR) and 8 plants around of each one reference-plant were evaluated, totaling 36 plants for variety. The detection of the bacteria was conduced by Polimerase Chain Reaction (PCR) technique. Simultaneously, evaluations through visual symptoms of CVC were conducted. The results of the temporal analysis were obtained using the Monomolecular, Logistic and Gompertz models. The Logistic model was the one that best fit to describe the behavior of the disease in the time, for all the studied varieties. Data showed that the space behavior of the disease differed when the same area was evaluated by visual and molecular methods, resulting in a difference in the space pattern of the evaluated areas. Therefore, the two used methods, symptoms and PCR, were capable of verifying these changes in the presented space pattern, but the molecular analysis (PCR) was better at detecting the happened changes.
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spelling Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967Análise espaço-temporal da clorose variegada dos citros no Noroeste do Paraná, com uso de PCR para detecção de Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967diagnóstico molecularepidemiologiaXylella fastidiosa5.01.02.00-1 FitossanidadeCountless diseases affect the citriculture, as the citrus variegated chlorosis (CVC) which is caused by the bacteria Xylella fastidiosa. The aim of this work was to determine the space distribution of the disease inside commercial orchards in the Northwest of Paraná, using molecular methods. Symptomatic orchards were selected for CVC with the varieties 'Pêra', 'Valência' and 'Folha Murcha' (Citrus sinensis Osbeck). For each variety, 4 positive reference-plant for CVC (presence of CVC symptoms or molecular analysis by PCR) and 8 plants around of each one reference-plant were evaluated, totaling 36 plants for variety. The detection of the bacteria was conduced by Polimerase Chain Reaction (PCR) technique. Simultaneously, evaluations through visual symptoms of CVC were conducted. The results of the temporal analysis were obtained using the Monomolecular, Logistic and Gompertz models. The Logistic model was the one that best fit to describe the behavior of the disease in the time, for all the studied varieties. Data showed that the space behavior of the disease differed when the same area was evaluated by visual and molecular methods, resulting in a difference in the space pattern of the evaluated areas. Therefore, the two used methods, symptoms and PCR, were capable of verifying these changes in the presented space pattern, but the molecular analysis (PCR) was better at detecting the happened changes.A citricultura é afetada por inúmeras doenças, como a clorose variegada do citros (CVC), causada pela bactéria Xylella fastidiosa. O objetivo deste trabalho foi determinar a distribuição espacial da doença dentro de pomares comerciais do Noroeste do Paraná com o uso de métodos moleculares. Foram selecionados pomares sintomáticos para CVC com as variedades ‘Pêra’, ‘Valência’ e ‘Folha Murcha’ (Citrus sinensis Osbeck). Foram marcadas para cada variedade, 4 plantas-referência positivas para CVC (por sintomas e análise molecular) e 8 plantas ao redor de cada uma das plantas-referência foram amostradas, num total de 36 plantas por variedade. Realizou-se o teste da Reação da Polimerase em Cadeia (PCR) para detecção da bactéria e na mesma época foram conduzidas avaliações visuais de sintomas de CVC. Os resultados da análise temporal, utilizando-se os modelos Monomolecular, Logístico e Gompertz, apontaram o modelo Logístico como o que melhor se ajustou para descrever o comportamento da doença no tempo, para todas as variedades estudadas. Observou-se que o comportamento espacial da doença diferiu quando a mesma área foi avaliada pelos métodos visual e molecular, resultando em uma diferença no padrão espacial das áreas avaliadas. Portanto, ambos os métodos empregados, sintomas e PCR, foram capazes de constatar as mudanças no padrão espacial apresentado, sendo que a análise molecular (PCR) foi mais sensível para detectar as mudanças ocorridas.Universidade Estadual de Maringá2008-03-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/96710.4025/actasciagron.v28i3.967Acta Scientiarum. Agronomy; Vol 28 No 3 (2006); 421-425Acta Scientiarum. Agronomy; v. 28 n. 3 (2006); 421-4251807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/967/484Nunes, William Mário de CarvalhoZanutto, Carlos AlexandreCorazza-Nunes, Maria JúliaMolina, Rúbia de Oliveirainfo:eu-repo/semantics/openAccess2022-11-23T18:37:18Zoai:periodicos.uem.br/ojs:article/967Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:37:18Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
Análise espaço-temporal da clorose variegada dos citros no Noroeste do Paraná, com uso de PCR para detecção de Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
title Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
spellingShingle Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
Nunes, William Mário de Carvalho
diagnóstico molecular
epidemiologia
Xylella fastidiosa
5.01.02.00-1 Fitossanidade
title_short Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
title_full Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
title_fullStr Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
title_full_unstemmed Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
title_sort Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa - DOI: 10.4025/actasciagron.v28i3.967
author Nunes, William Mário de Carvalho
author_facet Nunes, William Mário de Carvalho
Zanutto, Carlos Alexandre
Corazza-Nunes, Maria Júlia
Molina, Rúbia de Oliveira
author_role author
author2 Zanutto, Carlos Alexandre
Corazza-Nunes, Maria Júlia
Molina, Rúbia de Oliveira
author2_role author
author
author
dc.contributor.author.fl_str_mv Nunes, William Mário de Carvalho
Zanutto, Carlos Alexandre
Corazza-Nunes, Maria Júlia
Molina, Rúbia de Oliveira
dc.subject.por.fl_str_mv diagnóstico molecular
epidemiologia
Xylella fastidiosa
5.01.02.00-1 Fitossanidade
topic diagnóstico molecular
epidemiologia
Xylella fastidiosa
5.01.02.00-1 Fitossanidade
description Countless diseases affect the citriculture, as the citrus variegated chlorosis (CVC) which is caused by the bacteria Xylella fastidiosa. The aim of this work was to determine the space distribution of the disease inside commercial orchards in the Northwest of Paraná, using molecular methods. Symptomatic orchards were selected for CVC with the varieties 'Pêra', 'Valência' and 'Folha Murcha' (Citrus sinensis Osbeck). For each variety, 4 positive reference-plant for CVC (presence of CVC symptoms or molecular analysis by PCR) and 8 plants around of each one reference-plant were evaluated, totaling 36 plants for variety. The detection of the bacteria was conduced by Polimerase Chain Reaction (PCR) technique. Simultaneously, evaluations through visual symptoms of CVC were conducted. The results of the temporal analysis were obtained using the Monomolecular, Logistic and Gompertz models. The Logistic model was the one that best fit to describe the behavior of the disease in the time, for all the studied varieties. Data showed that the space behavior of the disease differed when the same area was evaluated by visual and molecular methods, resulting in a difference in the space pattern of the evaluated areas. Therefore, the two used methods, symptoms and PCR, were capable of verifying these changes in the presented space pattern, but the molecular analysis (PCR) was better at detecting the happened changes.
publishDate 2008
dc.date.none.fl_str_mv 2008-03-04
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dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/967
10.4025/actasciagron.v28i3.967
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/967
identifier_str_mv 10.4025/actasciagron.v28i3.967
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dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/967/484
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 28 No 3 (2006); 421-425
Acta Scientiarum. Agronomy; v. 28 n. 3 (2006); 421-425
1807-8621
1679-9275
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