Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP

Detalhes bibliográficos
Autor(a) principal: Carraro, Bianca Pierina
Data de Publicação: 2008
Outros Autores: Nunes, William Mário de Carvalho, Corazza-Nunes, Maria Júlia, Machado, Marco Antonio, Stach-Machado, Dagmar Ruth
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1765
Resumo: Thirteen Citrus tristeza virus (CTV) strains from ‘Pera’ citrus orchards, collected in Northern Paraná State, Brazil, were evaluated and compared with two severe CTV strains from São Paulo State through I-DAS ELISA (Indirect Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) and SSCP (Single-strand Conformation Polymorphism) of virus capsid gene. In samples collected in two counties, high levels of absorbency (A405) were recorded, when monoclonal antibodies specific to CTV severe strains were used. In addition, SSCP patterns of these samples were identical to patterns recorded for the Capão Bonito complex from São Paulo State. These results suggest that citrus orchards of Northern Paraná State are infected with severe CTV strains.
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spelling Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCPAvaliação de complexos do Citrus tristeza virus da região Norte do Estado do Paraná por meio de testes imunológicos e SSCP do gene da capa proteica - DOI: 10.4025/actasciagron.v25i2.1765ELISACTVanticorpos monoclonais5.00.00.00-4 Ciências AgráriasThirteen Citrus tristeza virus (CTV) strains from ‘Pera’ citrus orchards, collected in Northern Paraná State, Brazil, were evaluated and compared with two severe CTV strains from São Paulo State through I-DAS ELISA (Indirect Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) and SSCP (Single-strand Conformation Polymorphism) of virus capsid gene. In samples collected in two counties, high levels of absorbency (A405) were recorded, when monoclonal antibodies specific to CTV severe strains were used. In addition, SSCP patterns of these samples were identical to patterns recorded for the Capão Bonito complex from São Paulo State. These results suggest that citrus orchards of Northern Paraná State are infected with severe CTV strains.Treze isolados do Citrus tristeza virus (CTV), obtidos de pomares de laranja ‘Pêra’, da região Norte do Paraná, foram avaliados e comparados a dois isolados severos do Estado de São Paulo, por meio de I-DAS ELISA (Indirect double antibody sandwich Enzyme Linked Immunosorbent Assay) e de SSCP (Single-strand conformation polymorphism) do gene da proteína do capsídeo do vírus. Extratos de plantas de dois municípios amostrados apresentaram consideráveis valores de absorbância (A405), com os anticorpos monoclonais específicos para estirpes severas do vírus, e padrões SSCP do gene da proteína do capsídeo semelhantes aos dos isolados, oriundos do Estado de São Paulo, obtidos de plantas inoculadas com o ‘complexo Capão Bonito’. Os resultados sugerem que os pomares citrícolas da região Norte do Paraná estejam infectados por variantes severos do vírus.Universidade Estadual de Maringá2008-04-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/176510.4025/actasciagron.v25i2.1765Acta Scientiarum. Agronomy; Vol 25 No 2 (2003); 269-273Acta Scientiarum. Agronomy; v. 25 n. 2 (2003); 269-2731807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1765/1588Carraro, Bianca PierinaNunes, William Mário de CarvalhoCorazza-Nunes, Maria JúliaMachado, Marco AntonioStach-Machado, Dagmar Ruthinfo:eu-repo/semantics/openAccess2022-11-23T18:37:30Zoai:periodicos.uem.br/ojs:article/1765Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:37:30Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
Avaliação de complexos do Citrus tristeza virus da região Norte do Estado do Paraná por meio de testes imunológicos e SSCP do gene da capa proteica - DOI: 10.4025/actasciagron.v25i2.1765
title Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
spellingShingle Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
Carraro, Bianca Pierina
ELISA
CTV
anticorpos monoclonais
5.00.00.00-4 Ciências Agrárias
title_short Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
title_full Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
title_fullStr Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
title_full_unstemmed Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
title_sort Evaluation of the Citrus tristeza virus complex occurrence in Northern Paraná State through immunological assay and SSCP
author Carraro, Bianca Pierina
author_facet Carraro, Bianca Pierina
Nunes, William Mário de Carvalho
Corazza-Nunes, Maria Júlia
Machado, Marco Antonio
Stach-Machado, Dagmar Ruth
author_role author
author2 Nunes, William Mário de Carvalho
Corazza-Nunes, Maria Júlia
Machado, Marco Antonio
Stach-Machado, Dagmar Ruth
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carraro, Bianca Pierina
Nunes, William Mário de Carvalho
Corazza-Nunes, Maria Júlia
Machado, Marco Antonio
Stach-Machado, Dagmar Ruth
dc.subject.por.fl_str_mv ELISA
CTV
anticorpos monoclonais
5.00.00.00-4 Ciências Agrárias
topic ELISA
CTV
anticorpos monoclonais
5.00.00.00-4 Ciências Agrárias
description Thirteen Citrus tristeza virus (CTV) strains from ‘Pera’ citrus orchards, collected in Northern Paraná State, Brazil, were evaluated and compared with two severe CTV strains from São Paulo State through I-DAS ELISA (Indirect Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) and SSCP (Single-strand Conformation Polymorphism) of virus capsid gene. In samples collected in two counties, high levels of absorbency (A405) were recorded, when monoclonal antibodies specific to CTV severe strains were used. In addition, SSCP patterns of these samples were identical to patterns recorded for the Capão Bonito complex from São Paulo State. These results suggest that citrus orchards of Northern Paraná State are infected with severe CTV strains.
publishDate 2008
dc.date.none.fl_str_mv 2008-04-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1765
10.4025/actasciagron.v25i2.1765
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1765
identifier_str_mv 10.4025/actasciagron.v25i2.1765
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1765/1588
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 25 No 2 (2003); 269-273
Acta Scientiarum. Agronomy; v. 25 n. 2 (2003); 269-273
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Agronomy (Online)
collection Acta Scientiarum. Agronomy (Online)
repository.name.fl_str_mv Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br
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