Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765 |
Resumo: | The species Caesalpinia pulcherrima, which belongs to the Fabaceae family, is frequently used as a hedge or street tree in urban landscapes, parks and gardens. It flowers profusely, and the flowers, depending on the variety, may be pink, orange or yellow. As flower characteristics are essential for determining the commercial value of ornamental species, the main objective of this work was to identify polymorphisms in C. pulcherrima plants that produce flowers of different colors using RAPD molecular markers. For this study, 30 adult plants were randomly chosen on streets located in Jaboticabal city, São Paulo State, Brazil, and leaf samples were collected for DNA extraction. Among these trees, 20 have orange flowers, eight have yellow flowers and only two produce pink flowers. From the 140 tested RAPD primers, 94 primers amplified defined fragments that resulted in 246 bands, which were 100% monomorphic. Thus, a polymorphism was not detected by any of the primers tested. It was concluded that the RAPD technique is not an efficient method for detecting polymorphisms and that more specific molecular markers should be tested. Additionally, the morphological characteristic “flower color” may be controlled by several genes or by the association of them. |
id |
UEM-5_4c12bfc363f360859715d2262f02607b |
---|---|
oai_identifier_str |
oai:periodicos.uem.br/ojs:article/13765 |
network_acronym_str |
UEM-5 |
network_name_str |
Acta Scientiarum. Agronomy (Online) |
repository_id_str |
|
spelling |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765peacock flowerflower colormonomorphismCiências AgráriasThe species Caesalpinia pulcherrima, which belongs to the Fabaceae family, is frequently used as a hedge or street tree in urban landscapes, parks and gardens. It flowers profusely, and the flowers, depending on the variety, may be pink, orange or yellow. As flower characteristics are essential for determining the commercial value of ornamental species, the main objective of this work was to identify polymorphisms in C. pulcherrima plants that produce flowers of different colors using RAPD molecular markers. For this study, 30 adult plants were randomly chosen on streets located in Jaboticabal city, São Paulo State, Brazil, and leaf samples were collected for DNA extraction. Among these trees, 20 have orange flowers, eight have yellow flowers and only two produce pink flowers. From the 140 tested RAPD primers, 94 primers amplified defined fragments that resulted in 246 bands, which were 100% monomorphic. Thus, a polymorphism was not detected by any of the primers tested. It was concluded that the RAPD technique is not an efficient method for detecting polymorphisms and that more specific molecular markers should be tested. Additionally, the morphological characteristic “flower color” may be controlled by several genes or by the association of them. Universidade Estadual de Maringá2011-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfimage/jpeghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1376510.4025/actasciagron.v34i3.13765Acta Scientiarum. Agronomy; Vol 34 No 3 (2012); 259-263Acta Scientiarum. Agronomy; v. 34 n. 3 (2012); 259-2631807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765/pdf_1http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765/751375141276Rodrigues, Maria Gabriela FontanettiMazzini, Renata BachinPivetta, Kathia Fernandes LopesAlves, Meire de CássiaDesidério, Janete Apparecidainfo:eu-repo/semantics/openAccess2022-11-23T18:38:30Zoai:periodicos.uem.br/ojs:article/13765Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:30Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 |
title |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 |
spellingShingle |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 Rodrigues, Maria Gabriela Fontanetti peacock flower flower color monomorphism Ciências Agrárias |
title_short |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 |
title_full |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 |
title_fullStr |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 |
title_full_unstemmed |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 |
title_sort |
Characterization of the genetic variability among Caesalpinia pulcherrima (L.) Sw. (Fabaceae) plants using RAPD molecular markers - doi: 10.4025/actasciagron.v34i3.13765 |
author |
Rodrigues, Maria Gabriela Fontanetti |
author_facet |
Rodrigues, Maria Gabriela Fontanetti Mazzini, Renata Bachin Pivetta, Kathia Fernandes Lopes Alves, Meire de Cássia Desidério, Janete Apparecida |
author_role |
author |
author2 |
Mazzini, Renata Bachin Pivetta, Kathia Fernandes Lopes Alves, Meire de Cássia Desidério, Janete Apparecida |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Maria Gabriela Fontanetti Mazzini, Renata Bachin Pivetta, Kathia Fernandes Lopes Alves, Meire de Cássia Desidério, Janete Apparecida |
dc.subject.por.fl_str_mv |
peacock flower flower color monomorphism Ciências Agrárias |
topic |
peacock flower flower color monomorphism Ciências Agrárias |
description |
The species Caesalpinia pulcherrima, which belongs to the Fabaceae family, is frequently used as a hedge or street tree in urban landscapes, parks and gardens. It flowers profusely, and the flowers, depending on the variety, may be pink, orange or yellow. As flower characteristics are essential for determining the commercial value of ornamental species, the main objective of this work was to identify polymorphisms in C. pulcherrima plants that produce flowers of different colors using RAPD molecular markers. For this study, 30 adult plants were randomly chosen on streets located in Jaboticabal city, São Paulo State, Brazil, and leaf samples were collected for DNA extraction. Among these trees, 20 have orange flowers, eight have yellow flowers and only two produce pink flowers. From the 140 tested RAPD primers, 94 primers amplified defined fragments that resulted in 246 bands, which were 100% monomorphic. Thus, a polymorphism was not detected by any of the primers tested. It was concluded that the RAPD technique is not an efficient method for detecting polymorphisms and that more specific molecular markers should be tested. Additionally, the morphological characteristic “flower color” may be controlled by several genes or by the association of them. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765 10.4025/actasciagron.v34i3.13765 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765 |
identifier_str_mv |
10.4025/actasciagron.v34i3.13765 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765/pdf_1 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13765/751375141276 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf image/jpeg |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 34 No 3 (2012); 259-263 Acta Scientiarum. Agronomy; v. 34 n. 3 (2012); 259-263 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305908256243712 |