Reserve metabolism of stored and germinated Araucaria angustifolia seeds
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42707 |
Resumo: | Germination metabolism of recalcitrant seeds of Araucaria angustifolia is activated in storage, which complicates the seeds conservation and utilization. This study aimed to identify the changes in the reserve metabolites of A. angustifolia seeds throughout storage in order to understand the processes of hydrolysis caused by germination metabolism. Mature seeds were harvested in southern Brazil and stored in an ambient environment and cold chamber conditions. Biochemical analyses were performed for embryos and megagametophytes from seeds stored for 0, 15, 30, 45, and 90 days. Due to seeds being in advanced germination in storage, they were evaluated at 90 days in different early developmental categories: I – seeds with mature embryos, II – seeds with embryos showing apparent elongation along the embryonic axis, and III – seeds with root protrusion. Higher contents of carbohydrate, protein, and amino acids were observed in embryos compared to megagametophytes, and these metabolites were decreased after onset of germination, especially in the embryo tissue. Mobilization of metabolites in megagametophytes would probably increase in later stages of germination. It is suggested that such alterations are not due to deterioration of reserve components, but instead are based on seed metabolism, which remains active after harvest with hydrolysis of metabolites providing energy for germination. |
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Acta Scientiarum. Agronomy (Online) |
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Reserve metabolism of stored and germinated Araucaria angustifolia seeds Brazilian pine; conifer; protein profile; reserve mobilization.Germination metabolism of recalcitrant seeds of Araucaria angustifolia is activated in storage, which complicates the seeds conservation and utilization. This study aimed to identify the changes in the reserve metabolites of A. angustifolia seeds throughout storage in order to understand the processes of hydrolysis caused by germination metabolism. Mature seeds were harvested in southern Brazil and stored in an ambient environment and cold chamber conditions. Biochemical analyses were performed for embryos and megagametophytes from seeds stored for 0, 15, 30, 45, and 90 days. Due to seeds being in advanced germination in storage, they were evaluated at 90 days in different early developmental categories: I – seeds with mature embryos, II – seeds with embryos showing apparent elongation along the embryonic axis, and III – seeds with root protrusion. Higher contents of carbohydrate, protein, and amino acids were observed in embryos compared to megagametophytes, and these metabolites were decreased after onset of germination, especially in the embryo tissue. Mobilization of metabolites in megagametophytes would probably increase in later stages of germination. It is suggested that such alterations are not due to deterioration of reserve components, but instead are based on seed metabolism, which remains active after harvest with hydrolysis of metabolites providing energy for germination.Universidade Estadual de Maringá2019-09-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4270710.4025/actasciagron.v41i1.42707Acta Scientiarum. Agronomy; Vol 41 (2019): Publicação Contínua; e42707Acta Scientiarum. Agronomy; v. 41 (2019): Publicação Contínua; e427071807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42707/751375148349Copyright (c) 2019 Acta Scientiarum. Agronomyhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAraldi, Cristhyane Garcia Coelho, Cileide Maria Medeiros2022-02-20T21:47:08Zoai:periodicos.uem.br/ojs:article/42707Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-02-20T21:47:08Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds |
title |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds |
spellingShingle |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds Araldi, Cristhyane Garcia Brazilian pine; conifer; protein profile; reserve mobilization. |
title_short |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds |
title_full |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds |
title_fullStr |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds |
title_full_unstemmed |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds |
title_sort |
Reserve metabolism of stored and germinated Araucaria angustifolia seeds |
author |
Araldi, Cristhyane Garcia |
author_facet |
Araldi, Cristhyane Garcia Coelho, Cileide Maria Medeiros |
author_role |
author |
author2 |
Coelho, Cileide Maria Medeiros |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Araldi, Cristhyane Garcia Coelho, Cileide Maria Medeiros |
dc.subject.por.fl_str_mv |
Brazilian pine; conifer; protein profile; reserve mobilization. |
topic |
Brazilian pine; conifer; protein profile; reserve mobilization. |
description |
Germination metabolism of recalcitrant seeds of Araucaria angustifolia is activated in storage, which complicates the seeds conservation and utilization. This study aimed to identify the changes in the reserve metabolites of A. angustifolia seeds throughout storage in order to understand the processes of hydrolysis caused by germination metabolism. Mature seeds were harvested in southern Brazil and stored in an ambient environment and cold chamber conditions. Biochemical analyses were performed for embryos and megagametophytes from seeds stored for 0, 15, 30, 45, and 90 days. Due to seeds being in advanced germination in storage, they were evaluated at 90 days in different early developmental categories: I – seeds with mature embryos, II – seeds with embryos showing apparent elongation along the embryonic axis, and III – seeds with root protrusion. Higher contents of carbohydrate, protein, and amino acids were observed in embryos compared to megagametophytes, and these metabolites were decreased after onset of germination, especially in the embryo tissue. Mobilization of metabolites in megagametophytes would probably increase in later stages of germination. It is suggested that such alterations are not due to deterioration of reserve components, but instead are based on seed metabolism, which remains active after harvest with hydrolysis of metabolites providing energy for germination. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42707 10.4025/actasciagron.v41i1.42707 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42707 |
identifier_str_mv |
10.4025/actasciagron.v41i1.42707 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42707/751375148349 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 41 (2019): Publicação Contínua; e42707 Acta Scientiarum. Agronomy; v. 41 (2019): Publicação Contínua; e42707 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305911225810944 |