Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
DOI: | 10.4025/actasciagron.v33i2.6025 |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6025 |
Resumo: | This research had the objective of standardizing the methodology for preconditioning of castor bean (Ricinus communis L.) seeds for the evaluation of their physiological potential by the tetrazolium test. The evaluated seed preconditioning methods were: seeds with coat between moist paper towel at 30, 35 and 40°C for 6, 8, 10, 12, 14, 16 and 18 hours; and seeds without coat between moist paper towel; and seeds with coat immersed in water, at 25, 30, 35 and 40°C for 1, 2, 3, 4, 5 and 6 hours. After preconditioning, the seed coat was removed, the seeds were cut lengthwise, and immersed in tetrazolium solution at a concentration of 0.2% for 120 minutes at 35°C. The seeds’ germination percentage, moisture content before and after preconditioning, and staining uniformity after the tetrazolium were evaluated. The statistical design was completely randomized, and the means comparisons were accomplished by the Tukey test at 0.05 level of probability. It was concluded that castor bean seeds should be preconditioned with coat between moist paper towel at 35°C for 12 hours, so that the results of the tetrazolium test will be similar to those obtained in the germination test. |
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Acta Scientiarum. Agronomy (Online) |
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Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025Pré-condicionamento das sementes de mamoneira para o teste de tetrazólio - doi: 10.4025/actasciagron.v33i2.6025Viabilitycastor beanmethodologyphysiological potentialViabilidademamonametodologiapotencial fisiológicoAgronomiaFitotecniaProdução e Beneficiamento de SementesThis research had the objective of standardizing the methodology for preconditioning of castor bean (Ricinus communis L.) seeds for the evaluation of their physiological potential by the tetrazolium test. The evaluated seed preconditioning methods were: seeds with coat between moist paper towel at 30, 35 and 40°C for 6, 8, 10, 12, 14, 16 and 18 hours; and seeds without coat between moist paper towel; and seeds with coat immersed in water, at 25, 30, 35 and 40°C for 1, 2, 3, 4, 5 and 6 hours. After preconditioning, the seed coat was removed, the seeds were cut lengthwise, and immersed in tetrazolium solution at a concentration of 0.2% for 120 minutes at 35°C. The seeds’ germination percentage, moisture content before and after preconditioning, and staining uniformity after the tetrazolium were evaluated. The statistical design was completely randomized, and the means comparisons were accomplished by the Tukey test at 0.05 level of probability. It was concluded that castor bean seeds should be preconditioned with coat between moist paper towel at 35°C for 12 hours, so that the results of the tetrazolium test will be similar to those obtained in the germination test.O objetivo do trabalho foi padronizar a metodologia de pré-condicionamento das sementes de mamoneira (Ricinus communis L.) para a avaliação do potencial fisiológico pelo teste de tetrazólio. Testaram-se os seguintes métodos de pré-condicionamento: sementes com tegumento entre papel umedecido a 30, 35 e 40°C por 6, 8, 10, 12, 14, 16 e 18h; sementes sem tegumento entre papel umedecido e sementes com tegumento imersas em água a 25, 30, 35 e 40° C por 1, 2, 3, 4, 5 e 6h. Após o pré-condicionamento, removeu-se o tegumento das sementes, que foram cortadas no sentido do comprimento e imersas na solução de tetrazólio a 0,2%, por 120 min., a 35°C. Avaliaram-se a porcentagem de germinação das sementes, o teor de água, antes e após a embebição, e a uniformidade na coloração das sementes após o teste de tetrazólio. O delineamento experimental foi inteiramente casualizado, e a comparação de médias realizada pelo teste de Tukey a 5% de probabilidade. Concluiu-se que o pré-condicionamento para o teste de tetrazólio deve ser realizado nas sementes de mamoneira com tegumento, entre papel toalha umedecido, a 35°C por 12h para que os resultados desse teste assemelhem-se aos obtidos no teste de germinação.Universidade Estadual de Maringá2011-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/602510.4025/actasciagron.v33i2.6025Acta Scientiarum. Agronomy; Vol 33 No 2 (2011); 303-311Acta Scientiarum. Agronomy; v. 33 n. 2 (2011); 303-3111807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6025/6025Gaspar-Oliveira, Carolina MariaMartins, Cibele ChalitaNakagawa, Joãoinfo:eu-repo/semantics/openAccess2022-11-23T18:38:16Zoai:periodicos.uem.br/ojs:article/6025Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:16Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 Pré-condicionamento das sementes de mamoneira para o teste de tetrazólio - doi: 10.4025/actasciagron.v33i2.6025 |
title |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 |
spellingShingle |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 Gaspar-Oliveira, Carolina Maria Viability castor bean methodology physiological potential Viabilidade mamona metodologia potencial fisiológico Agronomia Fitotecnia Produção e Beneficiamento de Sementes Gaspar-Oliveira, Carolina Maria Viability castor bean methodology physiological potential Viabilidade mamona metodologia potencial fisiológico Agronomia Fitotecnia Produção e Beneficiamento de Sementes |
title_short |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 |
title_full |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 |
title_fullStr |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 |
title_full_unstemmed |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 |
title_sort |
Preconditioning of castor bean seeds for the tetrazolium test - doi: 10.4025/actasciagron.v33i2.6025 |
author |
Gaspar-Oliveira, Carolina Maria |
author_facet |
Gaspar-Oliveira, Carolina Maria Gaspar-Oliveira, Carolina Maria Martins, Cibele Chalita Nakagawa, João Martins, Cibele Chalita Nakagawa, João |
author_role |
author |
author2 |
Martins, Cibele Chalita Nakagawa, João |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Gaspar-Oliveira, Carolina Maria Martins, Cibele Chalita Nakagawa, João |
dc.subject.por.fl_str_mv |
Viability castor bean methodology physiological potential Viabilidade mamona metodologia potencial fisiológico Agronomia Fitotecnia Produção e Beneficiamento de Sementes |
topic |
Viability castor bean methodology physiological potential Viabilidade mamona metodologia potencial fisiológico Agronomia Fitotecnia Produção e Beneficiamento de Sementes |
description |
This research had the objective of standardizing the methodology for preconditioning of castor bean (Ricinus communis L.) seeds for the evaluation of their physiological potential by the tetrazolium test. The evaluated seed preconditioning methods were: seeds with coat between moist paper towel at 30, 35 and 40°C for 6, 8, 10, 12, 14, 16 and 18 hours; and seeds without coat between moist paper towel; and seeds with coat immersed in water, at 25, 30, 35 and 40°C for 1, 2, 3, 4, 5 and 6 hours. After preconditioning, the seed coat was removed, the seeds were cut lengthwise, and immersed in tetrazolium solution at a concentration of 0.2% for 120 minutes at 35°C. The seeds’ germination percentage, moisture content before and after preconditioning, and staining uniformity after the tetrazolium were evaluated. The statistical design was completely randomized, and the means comparisons were accomplished by the Tukey test at 0.05 level of probability. It was concluded that castor bean seeds should be preconditioned with coat between moist paper towel at 35°C for 12 hours, so that the results of the tetrazolium test will be similar to those obtained in the germination test. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6025 10.4025/actasciagron.v33i2.6025 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6025 |
identifier_str_mv |
10.4025/actasciagron.v33i2.6025 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6025/6025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 33 No 2 (2011); 303-311 Acta Scientiarum. Agronomy; v. 33 n. 2 (2011); 303-311 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1822218526031609856 |
dc.identifier.doi.none.fl_str_mv |
10.4025/actasciagron.v33i2.6025 |