Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257 |
Resumo: | No previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture levels (different concentrations of graphite or activate charcoal) and two illumination levels (14-hr photoperiod or continuous illumination) were evaluated. Three replicates of each combination were utilised. After six months of culture, the average numbers of induced buds and roots per seedling were recorded for each concentration of activated charcoal or graphite. Whereas the largest number of buds in C. bicolor seedlings occurred on media containing 6.0 or 7.5 g L-1 graphite, the largest number of roots occurred on media containing 6.0 g L-1 activated charcoal. In the hybrid ‘BLC Pastoral Innocence’, the largest number of buds and roots was reported in medium with 4.5 g L-1 activated charcoal. When using graphite in place of activated charcoal, we obtained disparate the results in root formation that suggest that graphite is not a recommended substitute for activated charcoal. |
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Acta Scientiarum. Agronomy (Online) |
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Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257activated charcoalgraphiteorchidsmicropropagationNo previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture levels (different concentrations of graphite or activate charcoal) and two illumination levels (14-hr photoperiod or continuous illumination) were evaluated. Three replicates of each combination were utilised. After six months of culture, the average numbers of induced buds and roots per seedling were recorded for each concentration of activated charcoal or graphite. Whereas the largest number of buds in C. bicolor seedlings occurred on media containing 6.0 or 7.5 g L-1 graphite, the largest number of roots occurred on media containing 6.0 g L-1 activated charcoal. In the hybrid ‘BLC Pastoral Innocence’, the largest number of buds and roots was reported in medium with 4.5 g L-1 activated charcoal. When using graphite in place of activated charcoal, we obtained disparate the results in root formation that suggest that graphite is not a recommended substitute for activated charcoal. Universidade Estadual de Maringá2011-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionlaboratory analysislaboratory analysisapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1225710.4025/actasciagron.v34i2.12257Acta Scientiarum. Agronomy; Vol 34 No 2 (2012); 157-161Acta Scientiarum. Agronomy; v. 34 n. 2 (2012); 157-1611807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257/pdf_1Prizão, Eliane CristinaGonçalves, Letícia de MenezesGutierre, Maria AuxiliadoraMangolin, Claudete AparecidaMachado, Maria de Fátima Pires da Silvainfo:eu-repo/semantics/openAccess2022-11-23T18:38:27Zoai:periodicos.uem.br/ojs:article/12257Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:27Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 |
title |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 |
spellingShingle |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 Prizão, Eliane Cristina activated charcoal graphite orchids micropropagation |
title_short |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 |
title_full |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 |
title_fullStr |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 |
title_full_unstemmed |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 |
title_sort |
Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 |
author |
Prizão, Eliane Cristina |
author_facet |
Prizão, Eliane Cristina Gonçalves, Letícia de Menezes Gutierre, Maria Auxiliadora Mangolin, Claudete Aparecida Machado, Maria de Fátima Pires da Silva |
author_role |
author |
author2 |
Gonçalves, Letícia de Menezes Gutierre, Maria Auxiliadora Mangolin, Claudete Aparecida Machado, Maria de Fátima Pires da Silva |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Prizão, Eliane Cristina Gonçalves, Letícia de Menezes Gutierre, Maria Auxiliadora Mangolin, Claudete Aparecida Machado, Maria de Fátima Pires da Silva |
dc.subject.por.fl_str_mv |
activated charcoal graphite orchids micropropagation |
topic |
activated charcoal graphite orchids micropropagation |
description |
No previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture levels (different concentrations of graphite or activate charcoal) and two illumination levels (14-hr photoperiod or continuous illumination) were evaluated. Three replicates of each combination were utilised. After six months of culture, the average numbers of induced buds and roots per seedling were recorded for each concentration of activated charcoal or graphite. Whereas the largest number of buds in C. bicolor seedlings occurred on media containing 6.0 or 7.5 g L-1 graphite, the largest number of roots occurred on media containing 6.0 g L-1 activated charcoal. In the hybrid ‘BLC Pastoral Innocence’, the largest number of buds and roots was reported in medium with 4.5 g L-1 activated charcoal. When using graphite in place of activated charcoal, we obtained disparate the results in root formation that suggest that graphite is not a recommended substitute for activated charcoal. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion laboratory analysis laboratory analysis |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257 10.4025/actasciagron.v34i2.12257 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257 |
identifier_str_mv |
10.4025/actasciagron.v34i2.12257 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 34 No 2 (2012); 157-161 Acta Scientiarum. Agronomy; v. 34 n. 2 (2012); 157-161 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1750315305840672768 |