Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603 |
Resumo: | The objectives of the present study were to construct a cowpea genetic map using the F2 population resulting from the cross IC-1 x BRS Marataoã, based on single nucleotide polymorphism (SNP) markers, and to map the cpi gene, with additional reference to introgression with the consensus map of species, aiming to identify markers for assisted selection to develop more efficient cultivars for BNF. The parents and 89 F2 plants were genotyped with 51,128 SNP markers, of which 910 polymorphic markers were used to construct the map. The results revealed 11 linkage groups, with an average of 82 markers per chromosome and average distance of 1.26 cM between markers. Recombination analysis of the SNPs indicated that markers 2_12850 and 2_00188, located in linkage group 11, flanked the cpi gene at a distance of 6.7 cM and 5.64 cM, respectively. The introgression of linkage group 11 with the cowpea reference map revealed short distances (from zero to 0.6 cM) for these markers, indicating a strong association with the cpi gene. The constructed map and cpi mapping provide basic information that can assist the genetic breeding of more efficient cowpea plants for BNF by marker-assisted selection. |
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Acta Scientiarum. Agronomy (Online) |
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Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpeaVigna unguiculatanitrogen fixationmarker-assisted selectionThe objectives of the present study were to construct a cowpea genetic map using the F2 population resulting from the cross IC-1 x BRS Marataoã, based on single nucleotide polymorphism (SNP) markers, and to map the cpi gene, with additional reference to introgression with the consensus map of species, aiming to identify markers for assisted selection to develop more efficient cultivars for BNF. The parents and 89 F2 plants were genotyped with 51,128 SNP markers, of which 910 polymorphic markers were used to construct the map. The results revealed 11 linkage groups, with an average of 82 markers per chromosome and average distance of 1.26 cM between markers. Recombination analysis of the SNPs indicated that markers 2_12850 and 2_00188, located in linkage group 11, flanked the cpi gene at a distance of 6.7 cM and 5.64 cM, respectively. The introgression of linkage group 11 with the cowpea reference map revealed short distances (from zero to 0.6 cM) for these markers, indicating a strong association with the cpi gene. The constructed map and cpi mapping provide basic information that can assist the genetic breeding of more efficient cowpea plants for BNF by marker-assisted selection.Universidade Estadual de Maringá2019-09-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4260310.4025/actasciagron.v41i1.42603Acta Scientiarum. Agronomy; Vol 41 (2019): Publicação Contínua; e42603Acta Scientiarum. Agronomy; v. 41 (2019): Publicação Contínua; e426031807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603/751375148340Copyright (c) 2019 Acta Scientiarum. Agronomyhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSeido, Sirando LimaFernandes Santos, Carlos Antonio2022-02-20T21:47:12Zoai:periodicos.uem.br/ojs:article/42603Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-02-20T21:47:12Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea |
title |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea |
spellingShingle |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea Seido, Sirando Lima Vigna unguiculata nitrogen fixation marker-assisted selection |
title_short |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea |
title_full |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea |
title_fullStr |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea |
title_full_unstemmed |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea |
title_sort |
Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea |
author |
Seido, Sirando Lima |
author_facet |
Seido, Sirando Lima Fernandes Santos, Carlos Antonio |
author_role |
author |
author2 |
Fernandes Santos, Carlos Antonio |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Seido, Sirando Lima Fernandes Santos, Carlos Antonio |
dc.subject.por.fl_str_mv |
Vigna unguiculata nitrogen fixation marker-assisted selection |
topic |
Vigna unguiculata nitrogen fixation marker-assisted selection |
description |
The objectives of the present study were to construct a cowpea genetic map using the F2 population resulting from the cross IC-1 x BRS Marataoã, based on single nucleotide polymorphism (SNP) markers, and to map the cpi gene, with additional reference to introgression with the consensus map of species, aiming to identify markers for assisted selection to develop more efficient cultivars for BNF. The parents and 89 F2 plants were genotyped with 51,128 SNP markers, of which 910 polymorphic markers were used to construct the map. The results revealed 11 linkage groups, with an average of 82 markers per chromosome and average distance of 1.26 cM between markers. Recombination analysis of the SNPs indicated that markers 2_12850 and 2_00188, located in linkage group 11, flanked the cpi gene at a distance of 6.7 cM and 5.64 cM, respectively. The introgression of linkage group 11 with the cowpea reference map revealed short distances (from zero to 0.6 cM) for these markers, indicating a strong association with the cpi gene. The constructed map and cpi mapping provide basic information that can assist the genetic breeding of more efficient cowpea plants for BNF by marker-assisted selection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603 10.4025/actasciagron.v41i1.42603 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603 |
identifier_str_mv |
10.4025/actasciagron.v41i1.42603 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603/751375148340 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 41 (2019): Publicação Contínua; e42603 Acta Scientiarum. Agronomy; v. 41 (2019): Publicação Contínua; e42603 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305910641754112 |