In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267

Detalhes bibliográficos
Autor(a) principal: Franzon, Rodrigo Cezar
Data de Publicação: 2007
Outros Autores: Raseira, Maria do Carmo Bassols, Wagner Júnior, Américo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/267
Resumo: The objective of the present work was to check the best medium and conditions for in vitro germination of pollen of Surinam cherry (Eugenia uniflora). Possibility of storage in freezer was also studied. The pollen was collected from flowers at balloon stage and immediately after anthesis in two populations of this species at Embrapa Clima Temperado Research Center, Pelotas, of state Rio Grande do Sul , Brazil. The tested media were the standard (10% sugar, plus 1% agar dissolved in distilled water), and the addition of two H3BO3 concentrations (0.65 mM and 1.3 mM) to this medium. Incubation temperatures of 20, 25 and 30ºC were tested. The best germination percentage was achieved after five hours incubating at 25°C. Boron did not influence the pollen germination of Surinam cherry. The pollen lost viability after 105 days of storage in freezer.
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spelling In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267Testes de germinação in vitro e armazenamento de pólen de pitangueira (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267Myrtaceaeviabilidade de pólenarmazenamentomeio de culturaboroThe objective of the present work was to check the best medium and conditions for in vitro germination of pollen of Surinam cherry (Eugenia uniflora). Possibility of storage in freezer was also studied. The pollen was collected from flowers at balloon stage and immediately after anthesis in two populations of this species at Embrapa Clima Temperado Research Center, Pelotas, of state Rio Grande do Sul , Brazil. The tested media were the standard (10% sugar, plus 1% agar dissolved in distilled water), and the addition of two H3BO3 concentrations (0.65 mM and 1.3 mM) to this medium. Incubation temperatures of 20, 25 and 30ºC were tested. The best germination percentage was achieved after five hours incubating at 25°C. Boron did not influence the pollen germination of Surinam cherry. The pollen lost viability after 105 days of storage in freezer.O objetivo do presente trabalho foi verificar qual o meio de cultura e quais as condições que deveriam ser utilizadas em testes de germinação in vitro do pólen de pitangueira (Eugenia uniflora), bem como verificar a possibilidade de armazenamento em freezer (-18°C). O pólen foi coletado de flores em estádio de balão e logo após a antese, em duas populações desta espécie, na Embrapa Clima Temperado, em Pelotas, Rio Grande do Sul. Os meios de cultura testados foram o padrão (testemunha) (10% de açúcar e 1% de ágar em água destilada) e este acrescido de duas concentrações de H3BO3 (0,65 mM e 1,3 mM). Foram testadas as temperaturas de incubação de 20, 25 e 30ºC. Cinco horas a 25°C, em meio de cultura básico, proporcionou as melhores médias de germinação in vitro. O boro não influenciou a germinação média de pólen de pitangueira. O pólen apresentou grande perda de viabilidade após 105 dias de armazenamento em freezer.Universidade Estadual de Maringá2007-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/26710.4025/actasciagron.v29i2.267Acta Scientiarum. Agronomy; Vol 29 No 2 (2007); 251-255Acta Scientiarum. Agronomy; v. 29 n. 2 (2007); 251-2551807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/267/118Franzon, Rodrigo CezarRaseira, Maria do Carmo BassolsWagner Júnior, Américoinfo:eu-repo/semantics/openAccess2022-11-23T18:37:03Zoai:periodicos.uem.br/ojs:article/267Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:37:03Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
Testes de germinação in vitro e armazenamento de pólen de pitangueira (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
title In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
spellingShingle In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
Franzon, Rodrigo Cezar
Myrtaceae
viabilidade de pólen
armazenamento
meio de cultura
boro
title_short In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
title_full In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
title_fullStr In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
title_full_unstemmed In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
title_sort In vitro germination and storage of Surinam cherry pollen (Eugenia uniflora L.) - DOI: 10.4025/actasciagron.v29i2.267
author Franzon, Rodrigo Cezar
author_facet Franzon, Rodrigo Cezar
Raseira, Maria do Carmo Bassols
Wagner Júnior, Américo
author_role author
author2 Raseira, Maria do Carmo Bassols
Wagner Júnior, Américo
author2_role author
author
dc.contributor.author.fl_str_mv Franzon, Rodrigo Cezar
Raseira, Maria do Carmo Bassols
Wagner Júnior, Américo
dc.subject.por.fl_str_mv Myrtaceae
viabilidade de pólen
armazenamento
meio de cultura
boro
topic Myrtaceae
viabilidade de pólen
armazenamento
meio de cultura
boro
description The objective of the present work was to check the best medium and conditions for in vitro germination of pollen of Surinam cherry (Eugenia uniflora). Possibility of storage in freezer was also studied. The pollen was collected from flowers at balloon stage and immediately after anthesis in two populations of this species at Embrapa Clima Temperado Research Center, Pelotas, of state Rio Grande do Sul , Brazil. The tested media were the standard (10% sugar, plus 1% agar dissolved in distilled water), and the addition of two H3BO3 concentrations (0.65 mM and 1.3 mM) to this medium. Incubation temperatures of 20, 25 and 30ºC were tested. The best germination percentage was achieved after five hours incubating at 25°C. Boron did not influence the pollen germination of Surinam cherry. The pollen lost viability after 105 days of storage in freezer.
publishDate 2007
dc.date.none.fl_str_mv 2007-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/267
10.4025/actasciagron.v29i2.267
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/267
identifier_str_mv 10.4025/actasciagron.v29i2.267
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/267/118
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 29 No 2 (2007); 251-255
Acta Scientiarum. Agronomy; v. 29 n. 2 (2007); 251-255
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Agronomy (Online)
collection Acta Scientiarum. Agronomy (Online)
repository.name.fl_str_mv Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br
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