Methods for zingiberene quantification in tomato seeking indirect selection of arthropod-pest-resistant plants

• Autor(a) principal: Freitas, Joelson Andre de
• Data de Publicação: 2008
• Outros Autores: Maluf, Wilson Roberto, Cardoso, Maria das Graças, Benities, Flavio Rodrigo Gandolfi
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Acta Scientiarum. Agronomy (Online)
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2823
• topic: Lycopersicon hirsutum var; hirsutum; cromatografia; determinação espectrofotométrica; aleloquímico; sesquiterpeno; 5.00.00.00-4 Ciências Agrárias

Two quantification methodologies of zingiberene were tested to establish a criterion for indirect selection of tomato plants resistant to arthropod pests. The tomato plants evaluated were: Lycopersicon esculentum ‘TOM-556’' (absence or a low zingiberene content), L. hirsutum var. hirsutum ‘PI-127826’ (high zingiberene content),- F1 TOM-556 x PI-127826, L. hirsutum var. glabratum ‘PI-134417’, L. esculentum ‘TOM-600’ (the last two with high contents of 2 - tridecanone), and Zingiber officinalis ‘ginger’ (species with high zingiberene content). The extraction of zingiberene was made in 4.21 cm2 of tomato plant leaflets and 0.15g of Z. officinalis root, in 2mL of hexane stirred for 40 seconds and quantified in spectrophotometer and high-performance liquid chromatography (HPLC), at 270 nm. These methodologies presented a positive correlation (r=0.85) indicating a good precision of quantification by spectrophotometer. A fast, cheap and nondestructive methodology for zingiberene quantification in tomato plants was established