Is curtobacterium wilt biocontrol temperature dependent?
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18018 |
Resumo: | Abiotic stress interferes with plant-microbial interactions, but some microorganisms may buffer this interference. We investigated the interaction between temperature and bacterial wilt (Curtobacterium flaccumfaciens pv. flaccumfaciens - Cff) biocontrol and the ability of Bacillus subtilis strain ALB629 to colonize bean seedlings, to inhibit pathogen growth and to use different C and N sources. B. subtilis ALB629rif, a mutant selected from the wild population of ALB629, was used to monitor plant colonization at 20°C and 30°C. ALB629rif was detected only in the plant roots (103.22 CFU g-1) at the lower temperature but colonized the roots, stems, and leaves (105.85, 104.48, and 104.01 CFU g-1), respectively, at 30ºC. The area under the disease progress curve was also different at the two tested temperatures (p < 0.01). Nevertheless, the disease reduction using ALB629rif - treated seeds was similar: 71% and 75%, respectively, at 20 and 30ºC (p < 0.01). A higher efficiency of C and N source utilization was observed at the higher temperature, but the antagonist inhibited Cff growth equally at either temperature in vitro. Based on our results, temperature interferes with pathogen and antagonist plant colonization, but the overall suppression of bacterial wilt appears to be stabilized by ALB629. |
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Acta Scientiarum. Agronomy (Online) |
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Is curtobacterium wilt biocontrol temperature dependent?seed treatmentPhaseolus vulgarisantibiosistemperaturerhizobacteriaPGPRFitopatologiaAbiotic stress interferes with plant-microbial interactions, but some microorganisms may buffer this interference. We investigated the interaction between temperature and bacterial wilt (Curtobacterium flaccumfaciens pv. flaccumfaciens - Cff) biocontrol and the ability of Bacillus subtilis strain ALB629 to colonize bean seedlings, to inhibit pathogen growth and to use different C and N sources. B. subtilis ALB629rif, a mutant selected from the wild population of ALB629, was used to monitor plant colonization at 20°C and 30°C. ALB629rif was detected only in the plant roots (103.22 CFU g-1) at the lower temperature but colonized the roots, stems, and leaves (105.85, 104.48, and 104.01 CFU g-1), respectively, at 30ºC. The area under the disease progress curve was also different at the two tested temperatures (p < 0.01). Nevertheless, the disease reduction using ALB629rif - treated seeds was similar: 71% and 75%, respectively, at 20 and 30ºC (p < 0.01). A higher efficiency of C and N source utilization was observed at the higher temperature, but the antagonist inhibited Cff growth equally at either temperature in vitro. Based on our results, temperature interferes with pathogen and antagonist plant colonization, but the overall suppression of bacterial wilt appears to be stabilized by ALB629. Universidade Estadual de Maringá2014-08-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1801810.4025/actasciagron.v36i4.18018Acta Scientiarum. Agronomy; Vol 36 No 4 (2014); 409-415Acta Scientiarum. Agronomy; v. 36 n. 4 (2014); 409-4151807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18018/pdf_34Martins, Samuel JulioMedeiros, Flavio Henrique VasconcelosSouza, Ricardo MagelaVilela, Laize Aparecida Ferreirainfo:eu-repo/semantics/openAccess2014-12-04T09:31:22Zoai:periodicos.uem.br/ojs:article/18018Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2014-12-04T09:31:22Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Is curtobacterium wilt biocontrol temperature dependent? |
title |
Is curtobacterium wilt biocontrol temperature dependent? |
spellingShingle |
Is curtobacterium wilt biocontrol temperature dependent? Martins, Samuel Julio seed treatment Phaseolus vulgaris antibiosis temperature rhizobacteria PGPR Fitopatologia |
title_short |
Is curtobacterium wilt biocontrol temperature dependent? |
title_full |
Is curtobacterium wilt biocontrol temperature dependent? |
title_fullStr |
Is curtobacterium wilt biocontrol temperature dependent? |
title_full_unstemmed |
Is curtobacterium wilt biocontrol temperature dependent? |
title_sort |
Is curtobacterium wilt biocontrol temperature dependent? |
author |
Martins, Samuel Julio |
author_facet |
Martins, Samuel Julio Medeiros, Flavio Henrique Vasconcelos Souza, Ricardo Magela Vilela, Laize Aparecida Ferreira |
author_role |
author |
author2 |
Medeiros, Flavio Henrique Vasconcelos Souza, Ricardo Magela Vilela, Laize Aparecida Ferreira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martins, Samuel Julio Medeiros, Flavio Henrique Vasconcelos Souza, Ricardo Magela Vilela, Laize Aparecida Ferreira |
dc.subject.por.fl_str_mv |
seed treatment Phaseolus vulgaris antibiosis temperature rhizobacteria PGPR Fitopatologia |
topic |
seed treatment Phaseolus vulgaris antibiosis temperature rhizobacteria PGPR Fitopatologia |
description |
Abiotic stress interferes with plant-microbial interactions, but some microorganisms may buffer this interference. We investigated the interaction between temperature and bacterial wilt (Curtobacterium flaccumfaciens pv. flaccumfaciens - Cff) biocontrol and the ability of Bacillus subtilis strain ALB629 to colonize bean seedlings, to inhibit pathogen growth and to use different C and N sources. B. subtilis ALB629rif, a mutant selected from the wild population of ALB629, was used to monitor plant colonization at 20°C and 30°C. ALB629rif was detected only in the plant roots (103.22 CFU g-1) at the lower temperature but colonized the roots, stems, and leaves (105.85, 104.48, and 104.01 CFU g-1), respectively, at 30ºC. The area under the disease progress curve was also different at the two tested temperatures (p < 0.01). Nevertheless, the disease reduction using ALB629rif - treated seeds was similar: 71% and 75%, respectively, at 20 and 30ºC (p < 0.01). A higher efficiency of C and N source utilization was observed at the higher temperature, but the antagonist inhibited Cff growth equally at either temperature in vitro. Based on our results, temperature interferes with pathogen and antagonist plant colonization, but the overall suppression of bacterial wilt appears to be stabilized by ALB629. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18018 10.4025/actasciagron.v36i4.18018 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18018 |
identifier_str_mv |
10.4025/actasciagron.v36i4.18018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18018/pdf_34 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 36 No 4 (2014); 409-415 Acta Scientiarum. Agronomy; v. 36 n. 4 (2014); 409-415 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305908854980608 |