In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984 |
Resumo: | Anacardium othonianum Rizz. is a medicinal plant species that is native to the Brazilian savannah. Adult plants are different from other genus members in this ecosystem due to their size, and efforts to locate the plants may lead to their extraction from the savannah and, frequently, plant death. Micropropagation has played a significant role in the propagation and preservation of specimens of several plant species; therefore, this study evaluated the effect of various salt concentrations and culture medium volumes on the in vitro cultivation of A. othonianum Rizz. Trial (I) evaluated two culture media (MS and WPM) and three salt concentrations (100, 50 and 25%) in a completely randomized design. Trial (II) evaluated two culture media and salt concentrations, MS (50%) and WPM (100%), and five medium volumes (10, 15, 20, 25 and 30 mL per test tube) as a 2 x 5 factorial in a completely randomized design. After 30 and 60 days of growth, the percentage of plantlet survival, average plantlet and leaf lengths and the average number of leaves and buds per explant were evaluated. The MS (50 and 25%) media and WPM (100 and 50%) media were the most effective for plantlet regeneration. The best responses were observed in 15- and 25-mL volumes of the MS (50%) medium. Therefore, the use of a 15-mL volume is suggested for greater medium economy. |
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Acta Scientiarum. Agronomy (Online) |
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In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984cashewtissue culturein vitro propagationProdução VegetalAnacardium othonianum Rizz. is a medicinal plant species that is native to the Brazilian savannah. Adult plants are different from other genus members in this ecosystem due to their size, and efforts to locate the plants may lead to their extraction from the savannah and, frequently, plant death. Micropropagation has played a significant role in the propagation and preservation of specimens of several plant species; therefore, this study evaluated the effect of various salt concentrations and culture medium volumes on the in vitro cultivation of A. othonianum Rizz. Trial (I) evaluated two culture media (MS and WPM) and three salt concentrations (100, 50 and 25%) in a completely randomized design. Trial (II) evaluated two culture media and salt concentrations, MS (50%) and WPM (100%), and five medium volumes (10, 15, 20, 25 and 30 mL per test tube) as a 2 x 5 factorial in a completely randomized design. After 30 and 60 days of growth, the percentage of plantlet survival, average plantlet and leaf lengths and the average number of leaves and buds per explant were evaluated. The MS (50 and 25%) media and WPM (100 and 50%) media were the most effective for plantlet regeneration. The best responses were observed in 15- and 25-mL volumes of the MS (50%) medium. Therefore, the use of a 15-mL volume is suggested for greater medium economy. Universidade Estadual de Maringá2011-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1098410.4025/actasciagron.v34i1.10984Acta Scientiarum. Agronomy; Vol 34 No 1 (2012); 77-83Acta Scientiarum. Agronomy; v. 34 n. 1 (2012); 77-831807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984/pdf_1Assis, Kerlley CristinaPereira, Flávia DionísioCabral, Juliana Silva RodriguesSilva, Fabiano GuimarãesSilva, José WaldemarSantos, Silvia Correa dosinfo:eu-repo/semantics/openAccess2022-11-23T18:38:25Zoai:periodicos.uem.br/ojs:article/10984Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:25Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 |
title |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 |
spellingShingle |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 Assis, Kerlley Cristina cashew tissue culture in vitro propagation Produção Vegetal |
title_short |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 |
title_full |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 |
title_fullStr |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 |
title_full_unstemmed |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 |
title_sort |
In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984 |
author |
Assis, Kerlley Cristina |
author_facet |
Assis, Kerlley Cristina Pereira, Flávia Dionísio Cabral, Juliana Silva Rodrigues Silva, Fabiano Guimarães Silva, José Waldemar Santos, Silvia Correa dos |
author_role |
author |
author2 |
Pereira, Flávia Dionísio Cabral, Juliana Silva Rodrigues Silva, Fabiano Guimarães Silva, José Waldemar Santos, Silvia Correa dos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Assis, Kerlley Cristina Pereira, Flávia Dionísio Cabral, Juliana Silva Rodrigues Silva, Fabiano Guimarães Silva, José Waldemar Santos, Silvia Correa dos |
dc.subject.por.fl_str_mv |
cashew tissue culture in vitro propagation Produção Vegetal |
topic |
cashew tissue culture in vitro propagation Produção Vegetal |
description |
Anacardium othonianum Rizz. is a medicinal plant species that is native to the Brazilian savannah. Adult plants are different from other genus members in this ecosystem due to their size, and efforts to locate the plants may lead to their extraction from the savannah and, frequently, plant death. Micropropagation has played a significant role in the propagation and preservation of specimens of several plant species; therefore, this study evaluated the effect of various salt concentrations and culture medium volumes on the in vitro cultivation of A. othonianum Rizz. Trial (I) evaluated two culture media (MS and WPM) and three salt concentrations (100, 50 and 25%) in a completely randomized design. Trial (II) evaluated two culture media and salt concentrations, MS (50%) and WPM (100%), and five medium volumes (10, 15, 20, 25 and 30 mL per test tube) as a 2 x 5 factorial in a completely randomized design. After 30 and 60 days of growth, the percentage of plantlet survival, average plantlet and leaf lengths and the average number of leaves and buds per explant were evaluated. The MS (50 and 25%) media and WPM (100 and 50%) media were the most effective for plantlet regeneration. The best responses were observed in 15- and 25-mL volumes of the MS (50%) medium. Therefore, the use of a 15-mL volume is suggested for greater medium economy. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984 10.4025/actasciagron.v34i1.10984 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984 |
identifier_str_mv |
10.4025/actasciagron.v34i1.10984 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 34 No 1 (2012); 77-83 Acta Scientiarum. Agronomy; v. 34 n. 1 (2012); 77-83 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305908174454784 |