The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria

Detalhes bibliográficos
Autor(a) principal: Oliveira-Longatti, Silvia Maria de
Data de Publicação: 2020
Outros Autores: Marra, Leandro Marciano, Carvalho, Teotonio Soares de, Moreira, Fatima Maria de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/44332
Resumo: Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.
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spelling The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteriaThe culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteriainsoluble phosphateplant growth-promoting rhizobacteriasolubilization halomicrodroptoothpickMicrobiologia do soloinsoluble phosphateplant growth-promoting rhizobacteriasolubilization halomicrodroptoothpickMicrobiologia do soloAgar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.Universidade Estadual de Maringá2020-05-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionMétodo de pesquisamétodo de pesquisaapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4433210.4025/actasciagron.v42i1.44332Acta Scientiarum. Agronomy; Vol 42 (2020): Publicação contínua; e44332Acta Scientiarum. Agronomy; v. 42 (2020): Publicação contínua; e443321807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/44332/751375150086Copyright (c) 2020 Acta Scientiarum. Agronomyhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira-Longatti, Silvia Maria deMarra, Leandro MarcianoCarvalho, Teotonio Soares deMoreira, Fatima Maria de Souza2020-11-16T18:51:02Zoai:periodicos.uem.br/ojs:article/44332Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2020-11-16T18:51:02Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
spellingShingle The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
Oliveira-Longatti, Silvia Maria de
insoluble phosphate
plant growth-promoting rhizobacteria
solubilization halo
microdrop
toothpick
Microbiologia do solo
insoluble phosphate
plant growth-promoting rhizobacteria
solubilization halo
microdrop
toothpick
Microbiologia do solo
title_short The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_full The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_fullStr The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_full_unstemmed The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_sort The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
author Oliveira-Longatti, Silvia Maria de
author_facet Oliveira-Longatti, Silvia Maria de
Marra, Leandro Marciano
Carvalho, Teotonio Soares de
Moreira, Fatima Maria de Souza
author_role author
author2 Marra, Leandro Marciano
Carvalho, Teotonio Soares de
Moreira, Fatima Maria de Souza
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira-Longatti, Silvia Maria de
Marra, Leandro Marciano
Carvalho, Teotonio Soares de
Moreira, Fatima Maria de Souza
dc.subject.por.fl_str_mv insoluble phosphate
plant growth-promoting rhizobacteria
solubilization halo
microdrop
toothpick
Microbiologia do solo
insoluble phosphate
plant growth-promoting rhizobacteria
solubilization halo
microdrop
toothpick
Microbiologia do solo
topic insoluble phosphate
plant growth-promoting rhizobacteria
solubilization halo
microdrop
toothpick
Microbiologia do solo
insoluble phosphate
plant growth-promoting rhizobacteria
solubilization halo
microdrop
toothpick
Microbiologia do solo
description Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Método de pesquisa
método de pesquisa
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/44332
10.4025/actasciagron.v42i1.44332
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/44332
identifier_str_mv 10.4025/actasciagron.v42i1.44332
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/44332/751375150086
dc.rights.driver.fl_str_mv Copyright (c) 2020 Acta Scientiarum. Agronomy
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Acta Scientiarum. Agronomy
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 42 (2020): Publicação contínua; e44332
Acta Scientiarum. Agronomy; v. 42 (2020): Publicação contínua; e44332
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Agronomy (Online)
collection Acta Scientiarum. Agronomy (Online)
repository.name.fl_str_mv Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br
_version_ 1799305911303405568

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