In vitro orchid propagation of Oncidium baueri (Orchidaceae) without the use of agar - DOI: 10.4025/actasciagron.v28i1.1672

• Autor(a) principal: Faria, Ricardo Tadeu de
• Data de Publicação: 2008
• Outros Autores: Dalio, Ronaldo José Durigan, Unemoto, Lilian Keiko, Silva, Geraldo Lopes da
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Acta Scientiarum. Agronomy (Online)
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1672
• topic: orquídeas; substratos; cultura de tecidos; biofábrica; redução de custos; Oncidium; 5.01.03.00-8 Fitotecnia

This research aims at studying the in vitro propagation of Oncidium baueri (Orchidaceae) without the use of agar as a solidifying agent of culture medium. The seeds were germinated in Murashige and Skoog medium (MS, 1962) modified with half of the macronutrients concentrations. When the seedlings reach an average height of 1 cm, they were subcultivated in 600 mL plastic flasks containing 200 mL of the same nutritive medium used for germination. The evaluated treatments were: 7 g of agar per liter + 1/2 MS medium liquid; chopped (polyurethane) foam + 1/2 MS medium liquid; sphagnum + 1/2 MS medium liquid; thick sand + 1/2MS medium liquid. The evaluated variables after 8 months of study were: aerial part height, tallest root length, sprout number, root number and total fresh mass. The statistics analysis demonstrates that the sphagnum or thick sand treatments are not indicated to substitute the agar. However, the treatment with chopped polyurethane foam showed an optimum rooting and vegetative development of seedlings, being, therefore, an efficient and less costly alternative to replace the agar in the in vitro propagation of Oncidium baueri.