Intraspecific discrimination of fish populations by fluorescence spectroscopy
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/48395 |
Resumo: | This study used the visible fluorescence signal of scales from Astyanax lacustris fishes to differentiate ten populations of streams in Ivinhema River Basin, Upper Paraná Basin, Brazil. Scales were removed from the humeral region of each fish and the fluorescence spectroscopy was carried out with two excitation wavelengths: at 360 nm (UV-A) and 405 nm. The broad emission covers all visible regions and it is related to the organic fraction of scale, basically composed from type I collagen. By interpreting the experimental fluorescence spectra with multivariate statistical analysis, it was possible to discriminate the investigated populations. By exciting the inner face of scales at 405 nm, for instance, the obtained Wilk’s lambda was 0.143, and the ten sampled streams could be statistically differentiated with 85.2% of explanation. This fluorescence interpretation exhibits very good correlation with the diet composition, which was also investigated for the same fishes from which the scales were removed. The applied methodology was capable to analyze the scales of A. lacustris, and to provide meaningful and enlightening results for the differentiation of populations. This methodology is very important for aquatic environmental study, mainly because small fishes, non-migratory or with small migration rate, can exhibit differences among habitats, as response to genetic isolation and adjustment to local conditions. |
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Acta scientiarum. Technology (Online) |
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Intraspecific discrimination of fish populations by fluorescence spectroscopyfluorescence spectroscopy; collagen fluorescence; Astyanax lacustris diet composition; fish scales; bioindicators.This study used the visible fluorescence signal of scales from Astyanax lacustris fishes to differentiate ten populations of streams in Ivinhema River Basin, Upper Paraná Basin, Brazil. Scales were removed from the humeral region of each fish and the fluorescence spectroscopy was carried out with two excitation wavelengths: at 360 nm (UV-A) and 405 nm. The broad emission covers all visible regions and it is related to the organic fraction of scale, basically composed from type I collagen. By interpreting the experimental fluorescence spectra with multivariate statistical analysis, it was possible to discriminate the investigated populations. By exciting the inner face of scales at 405 nm, for instance, the obtained Wilk’s lambda was 0.143, and the ten sampled streams could be statistically differentiated with 85.2% of explanation. This fluorescence interpretation exhibits very good correlation with the diet composition, which was also investigated for the same fishes from which the scales were removed. The applied methodology was capable to analyze the scales of A. lacustris, and to provide meaningful and enlightening results for the differentiation of populations. This methodology is very important for aquatic environmental study, mainly because small fishes, non-migratory or with small migration rate, can exhibit differences among habitats, as response to genetic isolation and adjustment to local conditions.Universidade Estadual De Maringá2021-02-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/4839510.4025/actascitechnol.v43i1.48395Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e48395Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e483951806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/48395/751375151680Copyright (c) 2021 Acta Scientiarum. Technologyhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLima, Dinorah Machado Vaz de Santana, Cristiane ÁvilaAndrade, Luis Humberto da CunhaSúarez, Yzel RondonLima, Sandro Marcio2021-03-22T14:19:59Zoai:periodicos.uem.br/ojs:article/48395Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2021-03-22T14:19:59Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Intraspecific discrimination of fish populations by fluorescence spectroscopy |
title |
Intraspecific discrimination of fish populations by fluorescence spectroscopy |
spellingShingle |
Intraspecific discrimination of fish populations by fluorescence spectroscopy Lima, Dinorah Machado Vaz de fluorescence spectroscopy; collagen fluorescence; Astyanax lacustris diet composition; fish scales; bioindicators. |
title_short |
Intraspecific discrimination of fish populations by fluorescence spectroscopy |
title_full |
Intraspecific discrimination of fish populations by fluorescence spectroscopy |
title_fullStr |
Intraspecific discrimination of fish populations by fluorescence spectroscopy |
title_full_unstemmed |
Intraspecific discrimination of fish populations by fluorescence spectroscopy |
title_sort |
Intraspecific discrimination of fish populations by fluorescence spectroscopy |
author |
Lima, Dinorah Machado Vaz de |
author_facet |
Lima, Dinorah Machado Vaz de Santana, Cristiane Ávila Andrade, Luis Humberto da Cunha Súarez, Yzel Rondon Lima, Sandro Marcio |
author_role |
author |
author2 |
Santana, Cristiane Ávila Andrade, Luis Humberto da Cunha Súarez, Yzel Rondon Lima, Sandro Marcio |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lima, Dinorah Machado Vaz de Santana, Cristiane Ávila Andrade, Luis Humberto da Cunha Súarez, Yzel Rondon Lima, Sandro Marcio |
dc.subject.por.fl_str_mv |
fluorescence spectroscopy; collagen fluorescence; Astyanax lacustris diet composition; fish scales; bioindicators. |
topic |
fluorescence spectroscopy; collagen fluorescence; Astyanax lacustris diet composition; fish scales; bioindicators. |
description |
This study used the visible fluorescence signal of scales from Astyanax lacustris fishes to differentiate ten populations of streams in Ivinhema River Basin, Upper Paraná Basin, Brazil. Scales were removed from the humeral region of each fish and the fluorescence spectroscopy was carried out with two excitation wavelengths: at 360 nm (UV-A) and 405 nm. The broad emission covers all visible regions and it is related to the organic fraction of scale, basically composed from type I collagen. By interpreting the experimental fluorescence spectra with multivariate statistical analysis, it was possible to discriminate the investigated populations. By exciting the inner face of scales at 405 nm, for instance, the obtained Wilk’s lambda was 0.143, and the ten sampled streams could be statistically differentiated with 85.2% of explanation. This fluorescence interpretation exhibits very good correlation with the diet composition, which was also investigated for the same fishes from which the scales were removed. The applied methodology was capable to analyze the scales of A. lacustris, and to provide meaningful and enlightening results for the differentiation of populations. This methodology is very important for aquatic environmental study, mainly because small fishes, non-migratory or with small migration rate, can exhibit differences among habitats, as response to genetic isolation and adjustment to local conditions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/48395 10.4025/actascitechnol.v43i1.48395 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/48395 |
identifier_str_mv |
10.4025/actascitechnol.v43i1.48395 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/48395/751375151680 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e48395 Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e48395 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315337341042688 |