Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343

Detalhes bibliográficos
Autor(a) principal: Ernandes, Fernanda Maria Pagane Guereschi
Data de Publicação: 2009
Outros Autores: Cruz, Crispin Humberto Garcia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta scientiarum. Technology (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/343
Resumo: Levan is an exopolysaccharide synthesized by several microorganisms during fermentation of a culture medium containing sucrose, yeast extract and minerals. This biopolymer has applications in the food segment as stabilizers, thickeners, as carriers for flavor and fragrances, as well as in the pharmaceutical segment as a hypocholesterolemic agent and for exhibiting antitumor activity. This work aimed to analyze the kinetic parameters for levan production. The microorganism used was Zymomonas mobilis CCT 4494, incubated in a synthetic medium containing 200.0 g L-1 of sucrose, in a rotary shaker at 200 rpm and 30°C. Samples were taken every 24h, during a period of 96h, in order to determine variations of pH, biomass, reducing sugar, total reducing sugar and levan formation. It was observed that yields of biomass and synthesized biopolymer in 24h were superior to those obtained in 48, 72 and 96h of fermentation
id UEM-6_79c0d02979bda730e41dd1ee969eda88
oai_identifier_str oai:periodicos.uem.br/ojs:article/343
network_acronym_str UEM-6
network_name_str Acta scientiarum. Technology (Online)
repository_id_str
spelling Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343Análise dos parâmetros cinéticos para produção de levana por Zymomonas mobilis utilizando fermentação submersa - DOI: 10.4025/actascitechnol.v31i1.343levanaZymomonas mobilisfermentação submersaexopolissacarídeobactériaparâmetros cinéticos.Levan is an exopolysaccharide synthesized by several microorganisms during fermentation of a culture medium containing sucrose, yeast extract and minerals. This biopolymer has applications in the food segment as stabilizers, thickeners, as carriers for flavor and fragrances, as well as in the pharmaceutical segment as a hypocholesterolemic agent and for exhibiting antitumor activity. This work aimed to analyze the kinetic parameters for levan production. The microorganism used was Zymomonas mobilis CCT 4494, incubated in a synthetic medium containing 200.0 g L-1 of sucrose, in a rotary shaker at 200 rpm and 30°C. Samples were taken every 24h, during a period of 96h, in order to determine variations of pH, biomass, reducing sugar, total reducing sugar and levan formation. It was observed that yields of biomass and synthesized biopolymer in 24h were superior to those obtained in 48, 72 and 96h of fermentationA levana é um exopolissacarídeo sintetizado por vários microrganismos durante a fermentação de um meio de cultura à base de sacarose, extrato de levedura e sais minerais. Este biopolímero pode ser usado tanto na área de alimentos, como estabilizante, espessante e fixador de cor e sabor, quanto na farmacêutica, como hipocolesterolêmico e anticarcinogênico. O artigo teve como objetivo analisar a influência da temperatura e do pH, em relação a diferentes tempos de fermentação, no desenvolvimento celular e na produção de levana, por fermentação submersa. O microrganismo utilizado foi Zymomonas mobilis CCT 4494, incubado num meio de cultura quimicamente definido, acrescido de 200,0 g L-1 de sacarose. Amostras do caldo de fermentação foram recolhidas a cada 24h, durante o período de 96h, para posteriormente serem submetidas aos métodos analíticos: pH, biomassa, teores de açúcares redutores e de açúcares redutores totais e da formação de levana. Observou-se que os rendimentos obtidos de biomassa e do biopolímero sintetizado, em 24h, foram superiores àqueles obtidos em 48, 72 e 96h de fermentação.Universidade Estadual De Maringá2009-04-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/34310.4025/actascitechnol.v31i1.343Acta Scientiarum. Technology; Vol 31 No 1 (2009); 35-41Acta Scientiarum. Technology; v. 31 n. 1 (2009); 35-411806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/343/343Ernandes, Fernanda Maria Pagane GuereschiCruz, Crispin Humberto Garciainfo:eu-repo/semantics/openAccess2024-05-17T13:02:26Zoai:periodicos.uem.br/ojs:article/343Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:02:26Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
Análise dos parâmetros cinéticos para produção de levana por Zymomonas mobilis utilizando fermentação submersa - DOI: 10.4025/actascitechnol.v31i1.343
title Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
spellingShingle Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
Ernandes, Fernanda Maria Pagane Guereschi
levana
Zymomonas mobilis
fermentação submersa
exopolissacarídeo
bactéria
parâmetros cinéticos.
title_short Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
title_full Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
title_fullStr Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
title_full_unstemmed Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
title_sort Analysis of kinetic parameters for levan production by Zymomonas mobilis using submerged fermentation - DOI: 10.4025/actascitechnol.v31i1.343
author Ernandes, Fernanda Maria Pagane Guereschi
author_facet Ernandes, Fernanda Maria Pagane Guereschi
Cruz, Crispin Humberto Garcia
author_role author
author2 Cruz, Crispin Humberto Garcia
author2_role author
dc.contributor.author.fl_str_mv Ernandes, Fernanda Maria Pagane Guereschi
Cruz, Crispin Humberto Garcia
dc.subject.por.fl_str_mv levana
Zymomonas mobilis
fermentação submersa
exopolissacarídeo
bactéria
parâmetros cinéticos.
topic levana
Zymomonas mobilis
fermentação submersa
exopolissacarídeo
bactéria
parâmetros cinéticos.
description Levan is an exopolysaccharide synthesized by several microorganisms during fermentation of a culture medium containing sucrose, yeast extract and minerals. This biopolymer has applications in the food segment as stabilizers, thickeners, as carriers for flavor and fragrances, as well as in the pharmaceutical segment as a hypocholesterolemic agent and for exhibiting antitumor activity. This work aimed to analyze the kinetic parameters for levan production. The microorganism used was Zymomonas mobilis CCT 4494, incubated in a synthetic medium containing 200.0 g L-1 of sucrose, in a rotary shaker at 200 rpm and 30°C. Samples were taken every 24h, during a period of 96h, in order to determine variations of pH, biomass, reducing sugar, total reducing sugar and levan formation. It was observed that yields of biomass and synthesized biopolymer in 24h were superior to those obtained in 48, 72 and 96h of fermentation
publishDate 2009
dc.date.none.fl_str_mv 2009-04-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/343
10.4025/actascitechnol.v31i1.343
url http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/343
identifier_str_mv 10.4025/actascitechnol.v31i1.343
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/343/343
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Technology; Vol 31 No 1 (2009); 35-41
Acta Scientiarum. Technology; v. 31 n. 1 (2009); 35-41
1806-2563
1807-8664
reponame:Acta scientiarum. Technology (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta scientiarum. Technology (Online)
collection Acta scientiarum. Technology (Online)
repository.name.fl_str_mv Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actatech@uem.br
_version_ 1799315331434414080

Registros relacionados