In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49082 |
Resumo: | The structural complexity of the agaran type-sulfated polysaccharides (SPs) found in Acanthophora muscoides limits its investigation as anticoagulant alternative to heparin which induces clot complications. This study was extended to evaluate the properties of a SPs fraction and its alkali/desulfated derivatives on an intrinsic pathway-induced thrombin generation (TG) continuous model using 60-fold diluted normal or serpins-depleted human plasma. 0.75 M NaCl-eluted SPs fraction by DEAE-cellulose chromatography containing sulfate (35.20%), total sugars (55.97%) and no proteins showed charge homogeneity and heterogeneous molecular weight by agarose/polyacrylamide gel electrophoresis, respectively, using sequential staining with toluidine blue and Stains-All. Fourier Transform Infrared spectroscopy confirmed agaran-structure. Intact fraction poorly acted on the activated partial thromboplastin time (3.10 IU) than heparin (193 IU), but there was a preponderance of the serpin-independent effect than serpin-dependent one in TG assay comparing both systems was continually recorded. Heparin abolished plasma TG, but was inactive in depleted human plasma. While desulfated derivative of the respective fraction anticipated and induced thrombin formation vs. untreated plasma. The results suggested that sulfated sugars residues in the sacharide units of the polymer appear to be important to attenuate TG in vitro. |
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In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generationanionic polymers; chemical modification; plasma systems; thrombosis.The structural complexity of the agaran type-sulfated polysaccharides (SPs) found in Acanthophora muscoides limits its investigation as anticoagulant alternative to heparin which induces clot complications. This study was extended to evaluate the properties of a SPs fraction and its alkali/desulfated derivatives on an intrinsic pathway-induced thrombin generation (TG) continuous model using 60-fold diluted normal or serpins-depleted human plasma. 0.75 M NaCl-eluted SPs fraction by DEAE-cellulose chromatography containing sulfate (35.20%), total sugars (55.97%) and no proteins showed charge homogeneity and heterogeneous molecular weight by agarose/polyacrylamide gel electrophoresis, respectively, using sequential staining with toluidine blue and Stains-All. Fourier Transform Infrared spectroscopy confirmed agaran-structure. Intact fraction poorly acted on the activated partial thromboplastin time (3.10 IU) than heparin (193 IU), but there was a preponderance of the serpin-independent effect than serpin-dependent one in TG assay comparing both systems was continually recorded. Heparin abolished plasma TG, but was inactive in depleted human plasma. While desulfated derivative of the respective fraction anticipated and induced thrombin formation vs. untreated plasma. The results suggested that sulfated sugars residues in the sacharide units of the polymer appear to be important to attenuate TG in vitro.Universidade Estadual De Maringá2021-02-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/4908210.4025/actascitechnol.v43i1.49082Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e49082Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e490821806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49082/751375151683Copyright (c) 2021 Acta Scientiarum. Technologyhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRodrigues, José Ariévilo GurgelQuinderé, Ana Luíza GomesBenevides, Norma Maria Barros2021-03-22T14:19:50Zoai:periodicos.uem.br/ojs:article/49082Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2021-03-22T14:19:50Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation |
title |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation |
spellingShingle |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation Rodrigues, José Ariévilo Gurgel anionic polymers; chemical modification; plasma systems; thrombosis. |
title_short |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation |
title_full |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation |
title_fullStr |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation |
title_full_unstemmed |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation |
title_sort |
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native and modified sulfated polysaccharide fraction on thrombin generation |
author |
Rodrigues, José Ariévilo Gurgel |
author_facet |
Rodrigues, José Ariévilo Gurgel Quinderé, Ana Luíza Gomes Benevides, Norma Maria Barros |
author_role |
author |
author2 |
Quinderé, Ana Luíza Gomes Benevides, Norma Maria Barros |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rodrigues, José Ariévilo Gurgel Quinderé, Ana Luíza Gomes Benevides, Norma Maria Barros |
dc.subject.por.fl_str_mv |
anionic polymers; chemical modification; plasma systems; thrombosis. |
topic |
anionic polymers; chemical modification; plasma systems; thrombosis. |
description |
The structural complexity of the agaran type-sulfated polysaccharides (SPs) found in Acanthophora muscoides limits its investigation as anticoagulant alternative to heparin which induces clot complications. This study was extended to evaluate the properties of a SPs fraction and its alkali/desulfated derivatives on an intrinsic pathway-induced thrombin generation (TG) continuous model using 60-fold diluted normal or serpins-depleted human plasma. 0.75 M NaCl-eluted SPs fraction by DEAE-cellulose chromatography containing sulfate (35.20%), total sugars (55.97%) and no proteins showed charge homogeneity and heterogeneous molecular weight by agarose/polyacrylamide gel electrophoresis, respectively, using sequential staining with toluidine blue and Stains-All. Fourier Transform Infrared spectroscopy confirmed agaran-structure. Intact fraction poorly acted on the activated partial thromboplastin time (3.10 IU) than heparin (193 IU), but there was a preponderance of the serpin-independent effect than serpin-dependent one in TG assay comparing both systems was continually recorded. Heparin abolished plasma TG, but was inactive in depleted human plasma. While desulfated derivative of the respective fraction anticipated and induced thrombin formation vs. untreated plasma. The results suggested that sulfated sugars residues in the sacharide units of the polymer appear to be important to attenuate TG in vitro. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49082 10.4025/actascitechnol.v43i1.49082 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49082 |
identifier_str_mv |
10.4025/actascitechnol.v43i1.49082 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49082/751375151683 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e49082 Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e49082 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315337368305664 |