Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/52011 |
Resumo: | This study proposes the preparation, characterization, and evaluation of the antimicrobial activity of silver nanoparticles (AgNPs). AgNPs were synthesized from the leaf extracts of plants from the Arecaceae family, which are abundant in the Amazon region. AgNPs were characterized using UV/Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS), and their minimum inhibitory concentrations (MIC) against the bacteria Escherichia coli and Staphylococcus aureus. AgNPs presented maximum absorbance between 420 and 430 nm, the mean diameter obtained by DLS ranged from 130.43 to 352.93 nm and the polydispersity index (PdI) ranged from 0.523 to 0.689. The surface charge measured by the Zeta potential was negative and ranged from -17.2 to -26.97 mV. FTIR analysis suggests that the phenolic compounds and/or proteins in the chemical composition of the plants studied may have been responsible for the reduction of Ag+ ions and stabilization of AgNPs. The morphology of AgNPs observed was largely spherical and presented some agglomerates. Transmission electron microscopy analyses showed polydispersed AgNPs without the formation of large agglomerates. The synthesized AgNPs presented homogeneity and rapid bioreduction. The concentration of AgNPs required to eliminate microorganisms by up to 90% was lower for Gram-negative bacteria (2.75 μg mL-1) than for Gram-positive bacteria (21.75 μg mL-1). In addition, AgNPs synthesized from plant species that are native to the Amazon proved to be promising, since they showed excellent antimicrobial activity against microorganisms of clinical interest. |
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Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae familySÍNTESIS VERDE: CARACTERIZACIÓN Y ACTIVIDAD BIOLÓGICA DE NANOPARTICULAS DE PLATA CON EXTRACTOS ACUOSOS DE PLANTAS DE LA FAMILIA ARECACEAESYNTHÈSE VERTE: CARACTÉRISATION ET ACTIVITÉ BIOLOGIQUE DES NANOPARTICULES D'ARGENT À L'AIDE D'EXTRAITS AQUEUX DE PLANTES DE LA FAMILLE ARECACEAEGreen synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae familyscanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy.scanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy.This study proposes the preparation, characterization, and evaluation of the antimicrobial activity of silver nanoparticles (AgNPs). AgNPs were synthesized from the leaf extracts of plants from the Arecaceae family, which are abundant in the Amazon region. AgNPs were characterized using UV/Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS), and their minimum inhibitory concentrations (MIC) against the bacteria Escherichia coli and Staphylococcus aureus. AgNPs presented maximum absorbance between 420 and 430 nm, the mean diameter obtained by DLS ranged from 130.43 to 352.93 nm and the polydispersity index (PdI) ranged from 0.523 to 0.689. The surface charge measured by the Zeta potential was negative and ranged from -17.2 to -26.97 mV. FTIR analysis suggests that the phenolic compounds and/or proteins in the chemical composition of the plants studied may have been responsible for the reduction of Ag+ ions and stabilization of AgNPs. The morphology of AgNPs observed was largely spherical and presented some agglomerates. Transmission electron microscopy analyses showed polydispersed AgNPs without the formation of large agglomerates. The synthesized AgNPs presented homogeneity and rapid bioreduction. The concentration of AgNPs required to eliminate microorganisms by up to 90% was lower for Gram-negative bacteria (2.75 μg mL-1) than for Gram-positive bacteria (21.75 μg mL-1). In addition, AgNPs synthesized from plant species that are native to the Amazon proved to be promising, since they showed excellent antimicrobial activity against microorganisms of clinical interest.This study proposes the preparation, characterization, and evaluation of the antimicrobial activity of silver nanoparticles (AgNPs). AgNPs were synthesized from the leaf extracts of plants from the Arecaceae family, which are abundant in the Amazon region. AgNPs were characterized using UV/Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS), and their minimum inhibitory concentrations (MIC) against the bacteria Escherichia coli and Staphylococcus aureus. AgNPs presented maximum absorbance between 420 and 430 nm, the mean diameter obtained by DLS ranged from 130.43 to 352.93 nm and the polydispersity index (PdI) ranged from 0.523 to 0.689. The surface charge measured by the Zeta potential was negative and ranged from -17.2 to -26.97 mV. FTIR analysis suggests that the phenolic compounds and/or proteins in the chemical composition of the plants studied may have been responsible for the reduction of Ag+ ions and stabilization of AgNPs. The morphology of AgNPs observed was largely spherical and presented some agglomerates. Transmission electron microscopy analyses showed polydispersed AgNPs without the formation of large agglomerates. The synthesized AgNPs presented homogeneity and rapid bioreduction. The concentration of AgNPs required to eliminate microorganisms by up to 90% was lower for Gram-negative bacteria (2.75 μg mL-1) than for Gram-positive bacteria (21.75 μg mL-1). In addition, AgNPs synthesized from plant species that are native to the Amazon proved to be promising, since they showed excellent antimicrobial activity against microorganisms of clinical interest.Universidade Estadual De Maringá2021-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/5201110.4025/actascitechnol.v43i1.52011Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e52011Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e520111806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/52011/751375152734Copyright (c) 2021 Acta Scientiarum. Technologyhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLima, Alan Kelbis Oliveira Vasconcelos, Arthur Abinader Kobayashi, Renata Katsuko TakayamaNakazato, Gerson Braga, Hugo de CamposTaube, Paulo Sérgio2021-11-05T19:01:43Zoai:periodicos.uem.br/ojs:article/52011Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2021-11-05T19:01:43Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family SÍNTESIS VERDE: CARACTERIZACIÓN Y ACTIVIDAD BIOLÓGICA DE NANOPARTICULAS DE PLATA CON EXTRACTOS ACUOSOS DE PLANTAS DE LA FAMILIA ARECACEAE SYNTHÈSE VERTE: CARACTÉRISATION ET ACTIVITÉ BIOLOGIQUE DES NANOPARTICULES D'ARGENT À L'AIDE D'EXTRAITS AQUEUX DE PLANTES DE LA FAMILLE ARECACEAE Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family |
title |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family |
spellingShingle |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family Lima, Alan Kelbis Oliveira scanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy. scanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy. |
title_short |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family |
title_full |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family |
title_fullStr |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family |
title_full_unstemmed |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family |
title_sort |
Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family |
author |
Lima, Alan Kelbis Oliveira |
author_facet |
Lima, Alan Kelbis Oliveira Vasconcelos, Arthur Abinader Kobayashi, Renata Katsuko Takayama Nakazato, Gerson Braga, Hugo de Campos Taube, Paulo Sérgio |
author_role |
author |
author2 |
Vasconcelos, Arthur Abinader Kobayashi, Renata Katsuko Takayama Nakazato, Gerson Braga, Hugo de Campos Taube, Paulo Sérgio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Alan Kelbis Oliveira Vasconcelos, Arthur Abinader Kobayashi, Renata Katsuko Takayama Nakazato, Gerson Braga, Hugo de Campos Taube, Paulo Sérgio |
dc.subject.por.fl_str_mv |
scanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy. scanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy. |
topic |
scanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy. scanning electron microscopy; dynamic light scattering; antimicrobial activity; transmission electron microscopy. |
description |
This study proposes the preparation, characterization, and evaluation of the antimicrobial activity of silver nanoparticles (AgNPs). AgNPs were synthesized from the leaf extracts of plants from the Arecaceae family, which are abundant in the Amazon region. AgNPs were characterized using UV/Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS), and their minimum inhibitory concentrations (MIC) against the bacteria Escherichia coli and Staphylococcus aureus. AgNPs presented maximum absorbance between 420 and 430 nm, the mean diameter obtained by DLS ranged from 130.43 to 352.93 nm and the polydispersity index (PdI) ranged from 0.523 to 0.689. The surface charge measured by the Zeta potential was negative and ranged from -17.2 to -26.97 mV. FTIR analysis suggests that the phenolic compounds and/or proteins in the chemical composition of the plants studied may have been responsible for the reduction of Ag+ ions and stabilization of AgNPs. The morphology of AgNPs observed was largely spherical and presented some agglomerates. Transmission electron microscopy analyses showed polydispersed AgNPs without the formation of large agglomerates. The synthesized AgNPs presented homogeneity and rapid bioreduction. The concentration of AgNPs required to eliminate microorganisms by up to 90% was lower for Gram-negative bacteria (2.75 μg mL-1) than for Gram-positive bacteria (21.75 μg mL-1). In addition, AgNPs synthesized from plant species that are native to the Amazon proved to be promising, since they showed excellent antimicrobial activity against microorganisms of clinical interest. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/52011 10.4025/actascitechnol.v43i1.52011 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/52011 |
identifier_str_mv |
10.4025/actascitechnol.v43i1.52011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/52011/751375152734 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e52011 Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e52011 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315337451143168 |