DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH

Detalhes bibliográficos
Autor(a) principal: Bibi, Iram
Data de Publicação: 2023
Outros Autores: Shah, Syed Waqar Hussain
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta scientiarum. Technology (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/60454
Resumo: The pH dependence of heparin and dextran sulfate aided release of DNA from its polyplexes with polyaziridine (PA) has been investigated using Quartz crystal microbalance with dissipation monitoring (QCM-D). The acoustic behavior of ternary systems indicated that the DNA decondensation was favored at physiological pH. When pH < 7.4, PA was significantly positively charged and ternary complexation occurred. When pH exceeded 7.4, very small proportion of DNA was released upon heparin addition due to marked reduction in cationic nature of PA. An increase in thickness at the interface due to adsorbed material at pH 9.0 showed the retention of heparin on the surface despite of negligible release of DNA. In terms of PA binding, heparin proved to be a better competitor than dextran sulfate.
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spelling DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pHDNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pHTransfection; heparin; dextran sulfate; QCM-D; acoustic sensingTransfection; heparin; dextran sulfate; QCM-D; acoustic sensingThe pH dependence of heparin and dextran sulfate aided release of DNA from its polyplexes with polyaziridine (PA) has been investigated using Quartz crystal microbalance with dissipation monitoring (QCM-D). The acoustic behavior of ternary systems indicated that the DNA decondensation was favored at physiological pH. When pH < 7.4, PA was significantly positively charged and ternary complexation occurred. When pH exceeded 7.4, very small proportion of DNA was released upon heparin addition due to marked reduction in cationic nature of PA. An increase in thickness at the interface due to adsorbed material at pH 9.0 showed the retention of heparin on the surface despite of negligible release of DNA. In terms of PA binding, heparin proved to be a better competitor than dextran sulfate.The pH dependence of heparin and dextran sulfate aided release of DNA from its polyplexes with polyaziridine (PA) has been investigated using Quartz crystal microbalance with dissipation monitoring (QCM-D). The acoustic behavior of ternary systems indicated that the DNA decondensation was favored at physiological pH. When pH < 7.4, PA was significantly positively charged and ternary complexation occurred. When pH exceeded 7.4, very small proportion of DNA was released upon heparin addition due to marked reduction in cationic nature of PA. An increase in thickness at the interface due to adsorbed material at pH 9.0 showed the retention of heparin on the surface despite of negligible release of DNA. In terms of PA binding, heparin proved to be a better competitor than dextran sulfate.Universidade Estadual De Maringá2023-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/6045410.4025/actascitechnol.v45i1.60454Acta Scientiarum. Technology; Vol 45 (2023): Publicação contínua; e60454Acta Scientiarum. Technology; v. 45 (2023): Publicação contínua; e604541806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/60454/751375156496Copyright (c) 2023 Acta Scientiarum. Technologyhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBibi, IramShah, Syed Waqar Hussain2023-10-20T12:44:27Zoai:periodicos.uem.br/ojs:article/60454Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2023-10-20T12:44:27Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
title DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
spellingShingle DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
Bibi, Iram
Transfection; heparin; dextran sulfate; QCM-D; acoustic sensing
Transfection; heparin; dextran sulfate; QCM-D; acoustic sensing
title_short DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
title_full DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
title_fullStr DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
title_full_unstemmed DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
title_sort DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH
author Bibi, Iram
author_facet Bibi, Iram
Shah, Syed Waqar Hussain
author_role author
author2 Shah, Syed Waqar Hussain
author2_role author
dc.contributor.author.fl_str_mv Bibi, Iram
Shah, Syed Waqar Hussain
dc.subject.por.fl_str_mv Transfection; heparin; dextran sulfate; QCM-D; acoustic sensing
Transfection; heparin; dextran sulfate; QCM-D; acoustic sensing
topic Transfection; heparin; dextran sulfate; QCM-D; acoustic sensing
Transfection; heparin; dextran sulfate; QCM-D; acoustic sensing
description The pH dependence of heparin and dextran sulfate aided release of DNA from its polyplexes with polyaziridine (PA) has been investigated using Quartz crystal microbalance with dissipation monitoring (QCM-D). The acoustic behavior of ternary systems indicated that the DNA decondensation was favored at physiological pH. When pH < 7.4, PA was significantly positively charged and ternary complexation occurred. When pH exceeded 7.4, very small proportion of DNA was released upon heparin addition due to marked reduction in cationic nature of PA. An increase in thickness at the interface due to adsorbed material at pH 9.0 showed the retention of heparin on the surface despite of negligible release of DNA. In terms of PA binding, heparin proved to be a better competitor than dextran sulfate.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/60454
10.4025/actascitechnol.v45i1.60454
url http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/60454
identifier_str_mv 10.4025/actascitechnol.v45i1.60454
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/60454/751375156496
dc.rights.driver.fl_str_mv Copyright (c) 2023 Acta Scientiarum. Technology
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Acta Scientiarum. Technology
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Technology; Vol 45 (2023): Publicação contínua; e60454
Acta Scientiarum. Technology; v. 45 (2023): Publicação contínua; e60454
1806-2563
1807-8664
reponame:Acta scientiarum. Technology (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta scientiarum. Technology (Online)
collection Acta scientiarum. Technology (Online)
repository.name.fl_str_mv Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actatech@uem.br
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