Thermostability of peroxidase from cabbage leaves

Detalhes bibliográficos
Autor(a) principal: Roling, Marlei Scariot
Data de Publicação: 2008
Outros Autores: Moura, Vagner Marques, Clemente, Edmar
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta scientiarum. Technology (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3002
Resumo: Extracts of soluble and ion-bound peroxidase enzyme were obtained from the cabbage leaf (Brassica oleracea, L. var. capitata). Phosphate buffer solution 100 mM, pH 6.0 was used. A non-linear behavior was detected at 65, 70, 75ºC. The soluble peroxidase extract was much more stable than the ion-bound one. When regeneration analysis was undertaken at 70ºC, the ion-bound peroxidase continued stable and showed no renaturation, whereas the soluble extract became inactivated.
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spelling Thermostability of peroxidase from cabbage leavesTermoestabilidade da peroxidase extraída da folha de repolhoperoxidaseBrassica oleracea L. var. capitatainativação1.06.00.00-0 QuímicaExtracts of soluble and ion-bound peroxidase enzyme were obtained from the cabbage leaf (Brassica oleracea, L. var. capitata). Phosphate buffer solution 100 mM, pH 6.0 was used. A non-linear behavior was detected at 65, 70, 75ºC. The soluble peroxidase extract was much more stable than the ion-bound one. When regeneration analysis was undertaken at 70ºC, the ion-bound peroxidase continued stable and showed no renaturation, whereas the soluble extract became inactivated.Extratos da enzima peroxidase solúvel e ionicamente ligada foram obtidos da folha do repolho (Brassica oleracea L., var. capitata). Para essa extração usou-se solução-tampão fosfato 100 mM, pH 6,0. Perante o tratamento térmico nas temperaturas de 65, 70, 75ºC, observou-se um comportamento não linear. O extrato solúvel de peroxidase apresentou-se particularmente mais estável no tratamento térmico que a peroxidase ionicamente ligada. O estudo da regeneração foi realizado a 70ºC. A peroxidase ionicamente ligada permaneceu estável, demostrando não haver renaturação, enquanto que o extrato solúvel perdeu atividade.Universidade Estadual De Maringá2008-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/300210.4025/actascitechnol.v22i0.3002Acta Scientiarum. Technology; Vol 22 (2000); 1157-1160Acta Scientiarum. Technology; v. 22 (2000); 1157-11601806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3002/2202Roling, Marlei ScariotMoura, Vagner MarquesClemente, Edmarinfo:eu-repo/semantics/openAccess2024-05-17T13:02:52Zoai:periodicos.uem.br/ojs:article/3002Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:02:52Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Thermostability of peroxidase from cabbage leaves
Termoestabilidade da peroxidase extraída da folha de repolho
title Thermostability of peroxidase from cabbage leaves
spellingShingle Thermostability of peroxidase from cabbage leaves
Roling, Marlei Scariot
peroxidase
Brassica oleracea L. var. capitata
inativação
1.06.00.00-0 Química
title_short Thermostability of peroxidase from cabbage leaves
title_full Thermostability of peroxidase from cabbage leaves
title_fullStr Thermostability of peroxidase from cabbage leaves
title_full_unstemmed Thermostability of peroxidase from cabbage leaves
title_sort Thermostability of peroxidase from cabbage leaves
author Roling, Marlei Scariot
author_facet Roling, Marlei Scariot
Moura, Vagner Marques
Clemente, Edmar
author_role author
author2 Moura, Vagner Marques
Clemente, Edmar
author2_role author
author
dc.contributor.author.fl_str_mv Roling, Marlei Scariot
Moura, Vagner Marques
Clemente, Edmar
dc.subject.por.fl_str_mv peroxidase
Brassica oleracea L. var. capitata
inativação
1.06.00.00-0 Química
topic peroxidase
Brassica oleracea L. var. capitata
inativação
1.06.00.00-0 Química
description Extracts of soluble and ion-bound peroxidase enzyme were obtained from the cabbage leaf (Brassica oleracea, L. var. capitata). Phosphate buffer solution 100 mM, pH 6.0 was used. A non-linear behavior was detected at 65, 70, 75ºC. The soluble peroxidase extract was much more stable than the ion-bound one. When regeneration analysis was undertaken at 70ºC, the ion-bound peroxidase continued stable and showed no renaturation, whereas the soluble extract became inactivated.
publishDate 2008
dc.date.none.fl_str_mv 2008-05-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3002
10.4025/actascitechnol.v22i0.3002
url http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3002
identifier_str_mv 10.4025/actascitechnol.v22i0.3002
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3002/2202
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Technology; Vol 22 (2000); 1157-1160
Acta Scientiarum. Technology; v. 22 (2000); 1157-1160
1806-2563
1807-8664
reponame:Acta scientiarum. Technology (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta scientiarum. Technology (Online)
collection Acta scientiarum. Technology (Online)
repository.name.fl_str_mv Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actatech@uem.br
_version_ 1799315332540661760

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