Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge

Detalhes bibliográficos
Autor(a) principal: Medeiros, Raphael Corrêa
Data de Publicação: 2018
Outros Autores: Daniel, Luiz Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta scientiarum. Technology (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/28709
Resumo: For the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer their viability in sludge samples from two sewage treatment plants. The method used consisted of three successive centrifugations followed by immunofluorescence assay and staining with propidium iodide. Samples were 100% (14/14) positive for cysts and 57.1% (8/14) for oocysts, with higher concentrations and mean viability above 75%. The change in the treatment of activated sludge, from extended aeration to conventional system, caused no difference in the concentration and viability of (oo)cysts. 
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spelling Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludgeCryptosporidiumGiardiaactivated sludgereuse.Engenharia SanitáriaEngenharia SanitáriaFor the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer their viability in sludge samples from two sewage treatment plants. The method used consisted of three successive centrifugations followed by immunofluorescence assay and staining with propidium iodide. Samples were 100% (14/14) positive for cysts and 57.1% (8/14) for oocysts, with higher concentrations and mean viability above 75%. The change in the treatment of activated sludge, from extended aeration to conventional system, caused no difference in the concentration and viability of (oo)cysts. Universidade Estadual De Maringá2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionmicrobiological analysismicrobiological analysisapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/2870910.4025/actascitechnol.v40i1.28709Acta Scientiarum. Technology; Vol 40 (2018): Publicação Contínua; e28709Acta Scientiarum. Technology; v. 40 (2018): Publicação Contínua; e287091806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/28709/pdfCopyright (c) 2017 Acta Scientiarum. Technologyinfo:eu-repo/semantics/openAccessMedeiros, Raphael CorrêaDaniel, Luiz Antonio2019-07-17T11:53:47Zoai:periodicos.uem.br/ojs:article/28709Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2019-07-17T11:53:47Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
title Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
spellingShingle Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
Medeiros, Raphael Corrêa
Cryptosporidium
Giardia
activated sludge
reuse.
Engenharia Sanitária
Engenharia Sanitária
title_short Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
title_full Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
title_fullStr Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
title_full_unstemmed Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
title_sort Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge
author Medeiros, Raphael Corrêa
author_facet Medeiros, Raphael Corrêa
Daniel, Luiz Antonio
author_role author
author2 Daniel, Luiz Antonio
author2_role author
dc.contributor.author.fl_str_mv Medeiros, Raphael Corrêa
Daniel, Luiz Antonio
dc.subject.por.fl_str_mv Cryptosporidium
Giardia
activated sludge
reuse.
Engenharia Sanitária
Engenharia Sanitária
topic Cryptosporidium
Giardia
activated sludge
reuse.
Engenharia Sanitária
Engenharia Sanitária
description For the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer their viability in sludge samples from two sewage treatment plants. The method used consisted of three successive centrifugations followed by immunofluorescence assay and staining with propidium iodide. Samples were 100% (14/14) positive for cysts and 57.1% (8/14) for oocysts, with higher concentrations and mean viability above 75%. The change in the treatment of activated sludge, from extended aeration to conventional system, caused no difference in the concentration and viability of (oo)cysts. 
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
microbiological analysis
microbiological analysis
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/28709
10.4025/actascitechnol.v40i1.28709
url http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/28709
identifier_str_mv 10.4025/actascitechnol.v40i1.28709
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/28709/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2017 Acta Scientiarum. Technology
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Acta Scientiarum. Technology
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Technology; Vol 40 (2018): Publicação Contínua; e28709
Acta Scientiarum. Technology; v. 40 (2018): Publicação Contínua; e28709
1806-2563
1807-8664
reponame:Acta scientiarum. Technology (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta scientiarum. Technology (Online)
collection Acta scientiarum. Technology (Online)
repository.name.fl_str_mv Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actatech@uem.br
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