Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854 |
Resumo: | Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments. |
id |
UEM-6_f61738c78d0478af0e87f4853bce6bf6 |
---|---|
oai_identifier_str |
oai:periodicos.uem.br/ojs:article/49854 |
network_acronym_str |
UEM-6 |
network_name_str |
Acta scientiarum. Technology (Online) |
repository_id_str |
|
spelling |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibioticsEnterococci and Bacilli from surface water: assessment of their resistance to copper and antibioticsheavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments.Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments.Universidade Estadual De Maringá2020-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/4985410.4025/actascitechnol.v43i1.49854Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e49854Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e498541806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854/751375150563Copyright (c) 2021 Acta Scientiarum. Technologyhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMaia, Luciana Furlaneto Bravo, Gabriela Batista GomesWatanabe, Alex Kiyomassa Batista, Nayara de Oliveira Furlaneto, Márcia Cristina 2020-10-09T12:48:13Zoai:periodicos.uem.br/ojs:article/49854Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2020-10-09T12:48:13Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics |
title |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics |
spellingShingle |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics Maia, Luciana Furlaneto heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4. heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4. |
title_short |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics |
title_full |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics |
title_fullStr |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics |
title_full_unstemmed |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics |
title_sort |
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics |
author |
Maia, Luciana Furlaneto |
author_facet |
Maia, Luciana Furlaneto Bravo, Gabriela Batista Gomes Watanabe, Alex Kiyomassa Batista, Nayara de Oliveira Furlaneto, Márcia Cristina |
author_role |
author |
author2 |
Bravo, Gabriela Batista Gomes Watanabe, Alex Kiyomassa Batista, Nayara de Oliveira Furlaneto, Márcia Cristina |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Maia, Luciana Furlaneto Bravo, Gabriela Batista Gomes Watanabe, Alex Kiyomassa Batista, Nayara de Oliveira Furlaneto, Márcia Cristina |
dc.subject.por.fl_str_mv |
heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4. heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4. |
topic |
heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4. heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4. |
description |
Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854 10.4025/actascitechnol.v43i1.49854 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854 |
identifier_str_mv |
10.4025/actascitechnol.v43i1.49854 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854/751375150563 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Technology http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e49854 Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e49854 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315337375645696 |