Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics

Detalhes bibliográficos
Autor(a) principal: Maia, Luciana Furlaneto
Data de Publicação: 2020
Outros Autores: Bravo, Gabriela Batista Gomes, Watanabe, Alex Kiyomassa, Batista, Nayara de Oliveira, Furlaneto, Márcia Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta scientiarum. Technology (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854
Resumo: Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments.
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spelling Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibioticsEnterococci and Bacilli from surface water: assessment of their resistance to copper and antibioticsheavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments.Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments.Universidade Estadual De Maringá2020-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/4985410.4025/actascitechnol.v43i1.49854Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e49854Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e498541806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854/751375150563Copyright (c) 2021 Acta Scientiarum. Technologyhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMaia, Luciana Furlaneto Bravo, Gabriela Batista GomesWatanabe, Alex Kiyomassa Batista, Nayara de Oliveira Furlaneto, Márcia Cristina 2020-10-09T12:48:13Zoai:periodicos.uem.br/ojs:article/49854Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2020-10-09T12:48:13Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
title Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
spellingShingle Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
Maia, Luciana Furlaneto
heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.
heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.
title_short Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
title_full Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
title_fullStr Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
title_full_unstemmed Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
title_sort Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics
author Maia, Luciana Furlaneto
author_facet Maia, Luciana Furlaneto
Bravo, Gabriela Batista Gomes
Watanabe, Alex Kiyomassa
Batista, Nayara de Oliveira
Furlaneto, Márcia Cristina
author_role author
author2 Bravo, Gabriela Batista Gomes
Watanabe, Alex Kiyomassa
Batista, Nayara de Oliveira
Furlaneto, Márcia Cristina
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Maia, Luciana Furlaneto
Bravo, Gabriela Batista Gomes
Watanabe, Alex Kiyomassa
Batista, Nayara de Oliveira
Furlaneto, Márcia Cristina
dc.subject.por.fl_str_mv heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.
heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.
topic heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.
heavy metal-resistant; Enterococcus sp; Bacillus sp; CuSO4.
description Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854
10.4025/actascitechnol.v43i1.49854
url http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854
identifier_str_mv 10.4025/actascitechnol.v43i1.49854
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/49854/751375150563
dc.rights.driver.fl_str_mv Copyright (c) 2021 Acta Scientiarum. Technology
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Acta Scientiarum. Technology
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Technology; Vol 43 (2021): Publicação contínua; e49854
Acta Scientiarum. Technology; v. 43 (2021): Publicação contínua; e49854
1806-2563
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reponame:Acta scientiarum. Technology (Online)
instname:Universidade Estadual de Maringá (UEM)
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instname_str Universidade Estadual de Maringá (UEM)
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institution UEM
reponame_str Acta scientiarum. Technology (Online)
collection Acta scientiarum. Technology (Online)
repository.name.fl_str_mv Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)
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