Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Animal Sciences (Online) |
Texto Completo: | https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/31910 |
Resumo: | The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight. |
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Acta Scientiarum. Animal Sciences (Online) |
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Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formationfish farmingmicrobial aggregatespathogenicity.The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight. Editora da Universidade Estadual de Maringá2016-08-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa de campo; análise laboratorialapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/3191010.4025/actascianimsci.v38i3.31910Acta Scientiarum. Animal Sciences; Vol 38 No 3 (2016); 233-241Acta Scientiarum. Animal Sciences; v. 38 n. 3 (2016); 233-2411807-86721806-2636reponame:Acta Scientiarum. Animal Sciences (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/31910/pdfCopyright (c) 2016 Acta Scientiarum. Animal Sciencesinfo:eu-repo/semantics/openAccessSilva, Jéssica Lucinda Saldanha daCavalcante, Davi de HolandaCarvalho, Fátima Cristiane Teles deVieira, Regine Helena Silva dos FernandesSá, Marcelo Vinícius do Carmo eSousa, Oscarina Viana de2022-02-17T21:50:42Zoai:periodicos.uem.br/ojs:article/31910Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/oaiactaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com1807-86721806-2636opendoar:2022-02-17T21:50:42Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation |
title |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation |
spellingShingle |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation Silva, Jéssica Lucinda Saldanha da fish farming microbial aggregates pathogenicity. |
title_short |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation |
title_full |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation |
title_fullStr |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation |
title_full_unstemmed |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation |
title_sort |
Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation |
author |
Silva, Jéssica Lucinda Saldanha da |
author_facet |
Silva, Jéssica Lucinda Saldanha da Cavalcante, Davi de Holanda Carvalho, Fátima Cristiane Teles de Vieira, Regine Helena Silva dos Fernandes Sá, Marcelo Vinícius do Carmo e Sousa, Oscarina Viana de |
author_role |
author |
author2 |
Cavalcante, Davi de Holanda Carvalho, Fátima Cristiane Teles de Vieira, Regine Helena Silva dos Fernandes Sá, Marcelo Vinícius do Carmo e Sousa, Oscarina Viana de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Jéssica Lucinda Saldanha da Cavalcante, Davi de Holanda Carvalho, Fátima Cristiane Teles de Vieira, Regine Helena Silva dos Fernandes Sá, Marcelo Vinícius do Carmo e Sousa, Oscarina Viana de |
dc.subject.por.fl_str_mv |
fish farming microbial aggregates pathogenicity. |
topic |
fish farming microbial aggregates pathogenicity. |
description |
The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion pesquisa de campo; análise laboratorial |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/31910 10.4025/actascianimsci.v38i3.31910 |
url |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/31910 |
identifier_str_mv |
10.4025/actascianimsci.v38i3.31910 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/31910/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Acta Scientiarum. Animal Sciences info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Acta Scientiarum. Animal Sciences |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Animal Sciences; Vol 38 No 3 (2016); 233-241 Acta Scientiarum. Animal Sciences; v. 38 n. 3 (2016); 233-241 1807-8672 1806-2636 reponame:Acta Scientiarum. Animal Sciences (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Animal Sciences (Online) |
collection |
Acta Scientiarum. Animal Sciences (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com |
_version_ |
1799315361752940544 |