Histology of branchiae of larvae of Nile tilapia, Oreochromis niloticus (L.) from Thailand, with different levels of vitamin C

• Autor(a) principal: Leonardo, Jussara Maria Leite Oliveira
• Data de Publicação: 2008
• Outros Autores: Vargas, Lauro, Ribeiro, Ricardo Pereira, Moreira, Heden Luiz Marques, Natali, Maria Raquel Marçal, Volski, Tarcila, Cavichiolo, Fabiana
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Acta Scientiarum. Animal Sciences (Online)
• Texto Completo: https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/2637
• topic: ácido ascórbico; histologia branquial; tilápia do Nilo; vitamina C; 5.04.00.00-2 Zootecnia

The effect of vitamin C (ascorbic acid) on the histological integrity and the morphology of mucous cells from gill structure of larvae of the Nile tilapia, Oreochromis niloticus (Perciformes, cichlidae), from Thailand, during sex reversion process was evaluated. Assay was performed during 28 days in Maringá PR Brazil. Three diets were given to the larvae: vitamin C-free diet; and two diets with monophosphated vitamin C, or rather, T1 (0); T2 (1,000); T3 (2,000) mg of vitamin C per kilo of diet respectively. Histological changes were classified according to severity levels in a scale ranging from 1 to 3 unities of Mean Assessment Value (MAV). These were epithelial lifting, hyperplasia of filament cells, telangectasy, and lamellar fusion, significantly more frequent in T1 than in T2 and T3. Morphometrical analyses showed an increase in average size of cells in T1 when compared to those of T2 or T3 (p < 0.05). Number of mucous cells showed a higher significant frequency of cells in T1 filaments as a contrast to those of T2 and T3. Results indicate that best inclusion level for larval phase of Nile tilapia from Thailand was 1000mg of vitamin C per kilo of diet.