Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Animal Sciences (Online) |
Texto Completo: | https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/56368 |
Resumo: | The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme. |
id |
UEM-7_bb569061409220bd0b5df5980ddaaf56 |
---|---|
oai_identifier_str |
oai:periodicos.uem.br/ojs:article/56368 |
network_acronym_str |
UEM-7 |
network_name_str |
Acta Scientiarum. Animal Sciences (Online) |
repository_id_str |
|
spelling |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)Anglo-Nubian; Lf gene; Philippine native goat; Saanen.Anglo-Nubian; Lf gene; Philippine native goat; Saanen.The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme.The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme.Editora da Universidade Estadual de Maringá2022-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/5636810.4025/actascianimsci.v44i1.56368Acta Scientiarum. Animal Sciences; Vol 44 (2022): Publicação contínua; e56368Acta Scientiarum. Animal Sciences; v. 44 (2022): Publicação contínua; e563681807-86721806-2636reponame:Acta Scientiarum. Animal Sciences (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/56368/751375154514Copyright (c) 2022 Acta Scientiarum. Animal Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessWaminal, Yancy Ogdamin Tubalinal, Gabriel Alexis San Pedro Mingala, Claro Niegos 2022-07-28T16:30:02Zoai:periodicos.uem.br/ojs:article/56368Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/oaiactaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com1807-86721806-2636opendoar:2022-07-28T16:30:02Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) |
title |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) |
spellingShingle |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) Waminal, Yancy Ogdamin Anglo-Nubian; Lf gene; Philippine native goat; Saanen. Anglo-Nubian; Lf gene; Philippine native goat; Saanen. |
title_short |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) |
title_full |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) |
title_fullStr |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) |
title_full_unstemmed |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) |
title_sort |
Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus) |
author |
Waminal, Yancy Ogdamin |
author_facet |
Waminal, Yancy Ogdamin Tubalinal, Gabriel Alexis San Pedro Mingala, Claro Niegos |
author_role |
author |
author2 |
Tubalinal, Gabriel Alexis San Pedro Mingala, Claro Niegos |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Waminal, Yancy Ogdamin Tubalinal, Gabriel Alexis San Pedro Mingala, Claro Niegos |
dc.subject.por.fl_str_mv |
Anglo-Nubian; Lf gene; Philippine native goat; Saanen. Anglo-Nubian; Lf gene; Philippine native goat; Saanen. |
topic |
Anglo-Nubian; Lf gene; Philippine native goat; Saanen. Anglo-Nubian; Lf gene; Philippine native goat; Saanen. |
description |
The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/56368 10.4025/actascianimsci.v44i1.56368 |
url |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/56368 |
identifier_str_mv |
10.4025/actascianimsci.v44i1.56368 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/56368/751375154514 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Acta Scientiarum. Animal Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Acta Scientiarum. Animal Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Animal Sciences; Vol 44 (2022): Publicação contínua; e56368 Acta Scientiarum. Animal Sciences; v. 44 (2022): Publicação contínua; e56368 1807-8672 1806-2636 reponame:Acta Scientiarum. Animal Sciences (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Animal Sciences (Online) |
collection |
Acta Scientiarum. Animal Sciences (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com |
_version_ |
1799315363723214848 |