HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Agricultura Neotropical |
Texto Completo: | https://periodicosonline.uems.br/index.php/agrineo/article/view/2899 |
Resumo: | This study aimed to evaluate the conidial production of Metarhizium rileyi in rice with different water volumes. The bioassay was composed by completely randomized design (CRD), with four treatments (20, 30, 40 and 50 mL of distilled water), being added 100 g of rice thin and long, making a total of 10 plastic bags per treatment, which were autoclaved for 15 minutes at 1.0 atm pressure, to 120 ºC. After the cooling of the rice, were added in each plastic bag, 2.0 mL of suspension containing 1 × 108 conidia mL-1. Then the bags were incubated for ten days in a germination chamber (BOD type) at 25 °C (±1 °C), 80% (±10%) relative humidity and 12h photoperiod to promote conidial germination and growth of the fungus, being performed a mild agitation every two days. The use of higher water volume resulted in greater conidial production and greater number of viable conidia. However, the highest rate of conidia germination was obtained with the use of 30 mL of water, is this the volume of water that corresponds to the best results. |
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HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUMThis study aimed to evaluate the conidial production of Metarhizium rileyi in rice with different water volumes. The bioassay was composed by completely randomized design (CRD), with four treatments (20, 30, 40 and 50 mL of distilled water), being added 100 g of rice thin and long, making a total of 10 plastic bags per treatment, which were autoclaved for 15 minutes at 1.0 atm pressure, to 120 ºC. After the cooling of the rice, were added in each plastic bag, 2.0 mL of suspension containing 1 × 108 conidia mL-1. Then the bags were incubated for ten days in a germination chamber (BOD type) at 25 °C (±1 °C), 80% (±10%) relative humidity and 12h photoperiod to promote conidial germination and growth of the fungus, being performed a mild agitation every two days. The use of higher water volume resulted in greater conidial production and greater number of viable conidia. However, the highest rate of conidia germination was obtained with the use of 30 mL of water, is this the volume of water that corresponds to the best results.Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Cassilândia2019-08-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicosonline.uems.br/index.php/agrineo/article/view/289910.32404/rean.v6i3.2899Revista de Agricultura Neotropical; v. 6 n. 3 (2019): Revista de Agricultura Neotropical; 48-52REVISTA DE AGRICULTURA NEOTROPICAL; Vol. 6 No. 3 (2019): REVISTA DE AGRICULTURA NEOTROPICAL; 48-522358-630310.32404/rean.v6i3reponame:Revista de Agricultura Neotropicalinstname:Universidade Estadual de Mato Grosso do Sul (UEMS)instacron:UEMSenghttps://periodicosonline.uems.br/index.php/agrineo/article/view/2899/2979Copyright (c) 2019 REVISTA DE AGRICULTURA NEOTROPICALinfo:eu-repo/semantics/openAccessLoureiro, Elisângela de SouzaPessoa, Luis Gustavo AmorimDias, Pamella MingottiRibeiro, Muller De PaulaTosta, Ricardo Alexandre De SouzaTeodoro, Paulo Eduardo2021-07-22T10:02:58ZRevistahttps://periodicosonline.uems.br/index.php/agrineoPUB |
dc.title.none.fl_str_mv |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM |
title |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM |
spellingShingle |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM Loureiro, Elisângela de Souza |
title_short |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM |
title_full |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM |
title_fullStr |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM |
title_full_unstemmed |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM |
title_sort |
HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM |
author |
Loureiro, Elisângela de Souza |
author_facet |
Loureiro, Elisângela de Souza Pessoa, Luis Gustavo Amorim Dias, Pamella Mingotti Ribeiro, Muller De Paula Tosta, Ricardo Alexandre De Souza Teodoro, Paulo Eduardo |
author_role |
author |
author2 |
Pessoa, Luis Gustavo Amorim Dias, Pamella Mingotti Ribeiro, Muller De Paula Tosta, Ricardo Alexandre De Souza Teodoro, Paulo Eduardo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Loureiro, Elisângela de Souza Pessoa, Luis Gustavo Amorim Dias, Pamella Mingotti Ribeiro, Muller De Paula Tosta, Ricardo Alexandre De Souza Teodoro, Paulo Eduardo |
description |
This study aimed to evaluate the conidial production of Metarhizium rileyi in rice with different water volumes. The bioassay was composed by completely randomized design (CRD), with four treatments (20, 30, 40 and 50 mL of distilled water), being added 100 g of rice thin and long, making a total of 10 plastic bags per treatment, which were autoclaved for 15 minutes at 1.0 atm pressure, to 120 ºC. After the cooling of the rice, were added in each plastic bag, 2.0 mL of suspension containing 1 × 108 conidia mL-1. Then the bags were incubated for ten days in a germination chamber (BOD type) at 25 °C (±1 °C), 80% (±10%) relative humidity and 12h photoperiod to promote conidial germination and growth of the fungus, being performed a mild agitation every two days. The use of higher water volume resulted in greater conidial production and greater number of viable conidia. However, the highest rate of conidia germination was obtained with the use of 30 mL of water, is this the volume of water that corresponds to the best results. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicosonline.uems.br/index.php/agrineo/article/view/2899 10.32404/rean.v6i3.2899 |
url |
https://periodicosonline.uems.br/index.php/agrineo/article/view/2899 |
identifier_str_mv |
10.32404/rean.v6i3.2899 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicosonline.uems.br/index.php/agrineo/article/view/2899/2979 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 REVISTA DE AGRICULTURA NEOTROPICAL info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 REVISTA DE AGRICULTURA NEOTROPICAL |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Cassilândia |
publisher.none.fl_str_mv |
Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Cassilândia |
dc.source.none.fl_str_mv |
Revista de Agricultura Neotropical; v. 6 n. 3 (2019): Revista de Agricultura Neotropical; 48-52 REVISTA DE AGRICULTURA NEOTROPICAL; Vol. 6 No. 3 (2019): REVISTA DE AGRICULTURA NEOTROPICAL; 48-52 2358-6303 10.32404/rean.v6i3 reponame:Revista de Agricultura Neotropical instname:Universidade Estadual de Mato Grosso do Sul (UEMS) instacron:UEMS |
instname_str |
Universidade Estadual de Mato Grosso do Sul (UEMS) |
instacron_str |
UEMS |
institution |
UEMS |
reponame_str |
Revista de Agricultura Neotropical |
collection |
Revista de Agricultura Neotropical |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
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1732994898772099072 |