In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
Texto Completo: | https://revista.uepb.edu.br/PBOCI/article/view/1120 |
Resumo: | Objective: To evaluate, in vitro, the effect of photodynamic therapy (PDT) compared to laser therapy and the use of a photosensitizer alone. Material and Methods: The following therapies were used: PDT, laser therapy and photosensitizer alone. For PDT, methylene blue (MB) at different concentrations and red laser InGaAlP 660nm were used. For the use of low-power laser (LPL) alone, red laser InGaAlP 660 nm and infrared laser AsGaAl, 830 nm, both in continuous emission were used. Standard ATCC strains of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) species were used. The antibacterial effect of PDT was quantified by the diameter of the inhibition halos. Results: PDT (LPL 660 nm, 320 J/cm2) with MB at concentration of 50 μg/mL showed antibacterial efficacy only when tested against S. aureus and E. coli strains, as well as with the isolated use of MB at the same concentration. Using LPL alone, whether red or infrared, with different dosimetry, no antibacterial effect was observed. In none of the therapeutic modalities used, P. aeruginosa inactivation was observed. Conclusion: Antibacterial effects of PDT (LPL 660 nm + MB 50 μg/mL) were observed for S. aureus and S. coli, as well as with the isolated use of MB (50 μg/mL). For P. aeruginosa, no antibacterial effect with any of the protocols recommended in the study was observed. |
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Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
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In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene BlueMicrobiologyBacteriaLow-Level Light TherapyPhotochemotherapyObjective: To evaluate, in vitro, the effect of photodynamic therapy (PDT) compared to laser therapy and the use of a photosensitizer alone. Material and Methods: The following therapies were used: PDT, laser therapy and photosensitizer alone. For PDT, methylene blue (MB) at different concentrations and red laser InGaAlP 660nm were used. For the use of low-power laser (LPL) alone, red laser InGaAlP 660 nm and infrared laser AsGaAl, 830 nm, both in continuous emission were used. Standard ATCC strains of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) species were used. The antibacterial effect of PDT was quantified by the diameter of the inhibition halos. Results: PDT (LPL 660 nm, 320 J/cm2) with MB at concentration of 50 μg/mL showed antibacterial efficacy only when tested against S. aureus and E. coli strains, as well as with the isolated use of MB at the same concentration. Using LPL alone, whether red or infrared, with different dosimetry, no antibacterial effect was observed. In none of the therapeutic modalities used, P. aeruginosa inactivation was observed. Conclusion: Antibacterial effects of PDT (LPL 660 nm + MB 50 μg/mL) were observed for S. aureus and S. coli, as well as with the isolated use of MB (50 μg/mL). For P. aeruginosa, no antibacterial effect with any of the protocols recommended in the study was observed.EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA2022-03-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.uepb.edu.br/PBOCI/article/view/1120Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 20 (2020); e4980Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 20 (2020); e49801983-46321519-0501reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)instname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBenghttps://revista.uepb.edu.br/PBOCI/article/view/1120/836Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integradahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCatão, Maria Helena Chaves de Vasconcelos Batista, Ana Luzia Araújo 2022-03-19T19:11:24Zoai:ojs.pkp.sfu.ca:article/1120Revistahttps://revista.uepb.edu.br/PBOCI/PUBhttps://revista.uepb.edu.br/PBOCI/oaipboci.editoria@gmail.com || alessandrouepb@gmail.com1983-46321519-0501opendoar:2022-03-19T19:11:24Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)false |
dc.title.none.fl_str_mv |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue |
title |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue |
spellingShingle |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue Catão, Maria Helena Chaves de Vasconcelos Microbiology Bacteria Low-Level Light Therapy Photochemotherapy |
title_short |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue |
title_full |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue |
title_fullStr |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue |
title_full_unstemmed |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue |
title_sort |
In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue |
author |
Catão, Maria Helena Chaves de Vasconcelos |
author_facet |
Catão, Maria Helena Chaves de Vasconcelos Batista, Ana Luzia Araújo |
author_role |
author |
author2 |
Batista, Ana Luzia Araújo |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Catão, Maria Helena Chaves de Vasconcelos Batista, Ana Luzia Araújo |
dc.subject.por.fl_str_mv |
Microbiology Bacteria Low-Level Light Therapy Photochemotherapy |
topic |
Microbiology Bacteria Low-Level Light Therapy Photochemotherapy |
description |
Objective: To evaluate, in vitro, the effect of photodynamic therapy (PDT) compared to laser therapy and the use of a photosensitizer alone. Material and Methods: The following therapies were used: PDT, laser therapy and photosensitizer alone. For PDT, methylene blue (MB) at different concentrations and red laser InGaAlP 660nm were used. For the use of low-power laser (LPL) alone, red laser InGaAlP 660 nm and infrared laser AsGaAl, 830 nm, both in continuous emission were used. Standard ATCC strains of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) species were used. The antibacterial effect of PDT was quantified by the diameter of the inhibition halos. Results: PDT (LPL 660 nm, 320 J/cm2) with MB at concentration of 50 μg/mL showed antibacterial efficacy only when tested against S. aureus and E. coli strains, as well as with the isolated use of MB at the same concentration. Using LPL alone, whether red or infrared, with different dosimetry, no antibacterial effect was observed. In none of the therapeutic modalities used, P. aeruginosa inactivation was observed. Conclusion: Antibacterial effects of PDT (LPL 660 nm + MB 50 μg/mL) were observed for S. aureus and S. coli, as well as with the isolated use of MB (50 μg/mL). For P. aeruginosa, no antibacterial effect with any of the protocols recommended in the study was observed. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.uepb.edu.br/PBOCI/article/view/1120 |
url |
https://revista.uepb.edu.br/PBOCI/article/view/1120 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revista.uepb.edu.br/PBOCI/article/view/1120/836 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integrada https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integrada https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA |
publisher.none.fl_str_mv |
EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA |
dc.source.none.fl_str_mv |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 20 (2020); e4980 Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 20 (2020); e4980 1983-4632 1519-0501 reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) instname:Universidade Estadual da Paraíba (UEPB) instacron:UEPB |
instname_str |
Universidade Estadual da Paraíba (UEPB) |
instacron_str |
UEPB |
institution |
UEPB |
reponame_str |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
collection |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
repository.name.fl_str_mv |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB) |
repository.mail.fl_str_mv |
pboci.editoria@gmail.com || alessandrouepb@gmail.com |
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1803387863405953024 |