In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue

Detalhes bibliográficos
Autor(a) principal: Catão, Maria Helena Chaves de Vasconcelos
Data de Publicação: 2022
Outros Autores: Batista, Ana Luzia Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
Texto Completo: https://revista.uepb.edu.br/PBOCI/article/view/1120
Resumo: Objective: To evaluate, in vitro, the effect of photodynamic therapy (PDT) compared to laser therapy and the use of a photosensitizer alone. Material and Methods: The following therapies were used: PDT, laser therapy and photosensitizer alone. For PDT, methylene blue (MB) at different concentrations and red laser InGaAlP 660nm were used. For the use of low-power laser (LPL) alone, red laser InGaAlP 660 nm and infrared laser AsGaAl, 830 nm, both in continuous emission were used. Standard ATCC strains of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) species were used. The antibacterial effect of PDT was quantified by the diameter of the inhibition halos. Results: PDT (LPL 660 nm, 320 J/cm2) with MB at concentration of 50 μg/mL showed antibacterial efficacy only when tested against S. aureus and E. coli strains, as well as with the isolated use of MB at the same concentration. Using LPL alone, whether red or infrared, with different dosimetry, no antibacterial effect was observed. In none of the therapeutic modalities used, P. aeruginosa inactivation was observed. Conclusion: Antibacterial effects of PDT (LPL 660 nm + MB 50 μg/mL) were observed for S. aureus and S. coli, as well as with the isolated use of MB (50 μg/mL). For P. aeruginosa, no antibacterial effect with any of the protocols recommended in the study was observed.
id UEPB-4_7f11fd0c15d3df2e2bd5e7e431edd75f
oai_identifier_str oai:ojs.pkp.sfu.ca:article/1120
network_acronym_str UEPB-4
network_name_str Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
repository_id_str
spelling In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene BlueMicrobiologyBacteriaLow-Level Light TherapyPhotochemotherapyObjective: To evaluate, in vitro, the effect of photodynamic therapy (PDT) compared to laser therapy and the use of a photosensitizer alone. Material and Methods: The following therapies were used: PDT, laser therapy and photosensitizer alone. For PDT, methylene blue (MB) at different concentrations and red laser InGaAlP 660nm were used. For the use of low-power laser (LPL) alone, red laser InGaAlP 660 nm and infrared laser AsGaAl, 830 nm, both in continuous emission were used. Standard ATCC strains of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) species were used. The antibacterial effect of PDT was quantified by the diameter of the inhibition halos. Results: PDT (LPL 660 nm, 320 J/cm2) with MB at concentration of 50 μg/mL showed antibacterial efficacy only when tested against S. aureus and E. coli strains, as well as with the isolated use of MB at the same concentration. Using LPL alone, whether red or infrared, with different dosimetry, no antibacterial effect was observed. In none of the therapeutic modalities used, P. aeruginosa inactivation was observed. Conclusion: Antibacterial effects of PDT (LPL 660 nm + MB 50 μg/mL) were observed for S. aureus and S. coli, as well as with the isolated use of MB (50 μg/mL). For P. aeruginosa, no antibacterial effect with any of the protocols recommended in the study was observed.EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA2022-03-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.uepb.edu.br/PBOCI/article/view/1120Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 20 (2020); e4980Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 20 (2020); e49801983-46321519-0501reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)instname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBenghttps://revista.uepb.edu.br/PBOCI/article/view/1120/836Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integradahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCatão, Maria Helena Chaves de Vasconcelos Batista, Ana Luzia Araújo 2022-03-19T19:11:24Zoai:ojs.pkp.sfu.ca:article/1120Revistahttps://revista.uepb.edu.br/PBOCI/PUBhttps://revista.uepb.edu.br/PBOCI/oaipboci.editoria@gmail.com || alessandrouepb@gmail.com1983-46321519-0501opendoar:2022-03-19T19:11:24Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)false
dc.title.none.fl_str_mv In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
title In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
spellingShingle In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
Catão, Maria Helena Chaves de Vasconcelos
Microbiology
Bacteria
Low-Level Light Therapy
Photochemotherapy
title_short In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
title_full In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
title_fullStr In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
title_full_unstemmed In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
title_sort In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
author Catão, Maria Helena Chaves de Vasconcelos
author_facet Catão, Maria Helena Chaves de Vasconcelos
Batista, Ana Luzia Araújo
author_role author
author2 Batista, Ana Luzia Araújo
author2_role author
dc.contributor.author.fl_str_mv Catão, Maria Helena Chaves de Vasconcelos
Batista, Ana Luzia Araújo
dc.subject.por.fl_str_mv Microbiology
Bacteria
Low-Level Light Therapy
Photochemotherapy
topic Microbiology
Bacteria
Low-Level Light Therapy
Photochemotherapy
description Objective: To evaluate, in vitro, the effect of photodynamic therapy (PDT) compared to laser therapy and the use of a photosensitizer alone. Material and Methods: The following therapies were used: PDT, laser therapy and photosensitizer alone. For PDT, methylene blue (MB) at different concentrations and red laser InGaAlP 660nm were used. For the use of low-power laser (LPL) alone, red laser InGaAlP 660 nm and infrared laser AsGaAl, 830 nm, both in continuous emission were used. Standard ATCC strains of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) species were used. The antibacterial effect of PDT was quantified by the diameter of the inhibition halos. Results: PDT (LPL 660 nm, 320 J/cm2) with MB at concentration of 50 μg/mL showed antibacterial efficacy only when tested against S. aureus and E. coli strains, as well as with the isolated use of MB at the same concentration. Using LPL alone, whether red or infrared, with different dosimetry, no antibacterial effect was observed. In none of the therapeutic modalities used, P. aeruginosa inactivation was observed. Conclusion: Antibacterial effects of PDT (LPL 660 nm + MB 50 μg/mL) were observed for S. aureus and S. coli, as well as with the isolated use of MB (50 μg/mL). For P. aeruginosa, no antibacterial effect with any of the protocols recommended in the study was observed.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/1120
url https://revista.uepb.edu.br/PBOCI/article/view/1120
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/1120/836
dc.rights.driver.fl_str_mv Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
dc.source.none.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 20 (2020); e4980
Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 20 (2020); e4980
1983-4632
1519-0501
reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
instname:Universidade Estadual da Paraíba (UEPB)
instacron:UEPB
instname_str Universidade Estadual da Paraíba (UEPB)
instacron_str UEPB
institution UEPB
reponame_str Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
collection Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
repository.name.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)
repository.mail.fl_str_mv pboci.editoria@gmail.com || alessandrouepb@gmail.com
_version_ 1803387863405953024