Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
Texto Completo: | https://revista.uepb.edu.br/PBOCI/article/view/668 |
Resumo: | Objective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments: genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test. Results: The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group. Conclusion: The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population. |
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Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
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Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial CleftCleft PalateCleft LipGenetic VariationPolymorphism, GeneticObjective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments: genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test. Results: The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group. Conclusion: The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population.EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA2021-11-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.uepb.edu.br/PBOCI/article/view/668Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 21 (2021); e5896Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 21 (2021); e58961983-46321519-0501reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)instname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBenghttps://revista.uepb.edu.br/PBOCI/article/view/668/362Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integradahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessAuerkari, Elza Ibrahim Bilynov, Yolanda Yuniastuti, Mindya Listyowati, Listyowati Sulistyani, Lilies Dwi 2021-11-13T17:38:31Zoai:ojs.pkp.sfu.ca:article/668Revistahttps://revista.uepb.edu.br/PBOCI/PUBhttps://revista.uepb.edu.br/PBOCI/oaipboci.editoria@gmail.com || alessandrouepb@gmail.com1983-46321519-0501opendoar:2021-11-13T17:38:31Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)false |
dc.title.none.fl_str_mv |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
title |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
spellingShingle |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft Auerkari, Elza Ibrahim Cleft Palate Cleft Lip Genetic Variation Polymorphism, Genetic |
title_short |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
title_full |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
title_fullStr |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
title_full_unstemmed |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
title_sort |
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
author |
Auerkari, Elza Ibrahim |
author_facet |
Auerkari, Elza Ibrahim Bilynov, Yolanda Yuniastuti, Mindya Listyowati, Listyowati Sulistyani, Lilies Dwi |
author_role |
author |
author2 |
Bilynov, Yolanda Yuniastuti, Mindya Listyowati, Listyowati Sulistyani, Lilies Dwi |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Auerkari, Elza Ibrahim Bilynov, Yolanda Yuniastuti, Mindya Listyowati, Listyowati Sulistyani, Lilies Dwi |
dc.subject.por.fl_str_mv |
Cleft Palate Cleft Lip Genetic Variation Polymorphism, Genetic |
topic |
Cleft Palate Cleft Lip Genetic Variation Polymorphism, Genetic |
description |
Objective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments: genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test. Results: The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group. Conclusion: The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.uepb.edu.br/PBOCI/article/view/668 |
url |
https://revista.uepb.edu.br/PBOCI/article/view/668 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revista.uepb.edu.br/PBOCI/article/view/668/362 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integrada https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integrada https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA |
publisher.none.fl_str_mv |
EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA |
dc.source.none.fl_str_mv |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 21 (2021); e5896 Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 21 (2021); e5896 1983-4632 1519-0501 reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) instname:Universidade Estadual da Paraíba (UEPB) instacron:UEPB |
instname_str |
Universidade Estadual da Paraíba (UEPB) |
instacron_str |
UEPB |
institution |
UEPB |
reponame_str |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
collection |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
repository.name.fl_str_mv |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB) |
repository.mail.fl_str_mv |
pboci.editoria@gmail.com || alessandrouepb@gmail.com |
_version_ |
1803387862387785728 |