Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells

Detalhes bibliográficos
Autor(a) principal: Kuntjoro, Mefina
Data de Publicação: 2024
Outros Autores: Hendrijantini, Nike, Prasetyo, Eric Priyo, Agustono, Bambang, Sitalaksmi, Ratri Maya, Hendrianto, Eryk, Dinaryanti, Aristika, Rusli, Marvin, Tjendronegoro, Evelyn, Hong, Guang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
Texto Completo: https://revista.uepb.edu.br/PBOCI/article/view/3123
Resumo: Objective: To assess the effects of cobalt chloride (CoCl2) as a hypoxia mimicking agent on human umbilical cord mesenchymal stem cells (hUCMSCs) expression of HIF-1α and mTOR for use in regenerative dentistry. Material and Methods: Human umbilical cord mesenchymal stem cells were isolated and then cultured. The characteristics of stemness were screened and confirmed by flow cytometry. The experiment was conducted on hypoxia (H) and normoxia (N) groups. Each group was divided and incubated into 24-, 48-, and 72-hours observations. Hypoxic treatment was performed using 100 µM CoCl2 on 5th passage cells in a conventional incubator (37°C; 5% CO2). Then, immunofluorescence of HIF-1α and mTOR was done. Data was analyzed statistically using One-way ANOVA and Tukey’s HSD. Results: Significant differences were found between normoxic and hypoxic groups on HIF-1α (p=0.015) and mTOR (p=0.000) expressions. The highest HIF-1α expression was found at 48 hours in the hypoxia group, while for mTOR at 24 hours in the hypoxia group. Conclusion: Hypoxia using cobalt chloride was able to increase human umbilical cord mesenchymal stem cells expression of HIF-1α and mTOR.
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spelling Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem CellsUmbilical CordMesenchymal Stem CellsStem Cell ResearchRegenerationObjective: To assess the effects of cobalt chloride (CoCl2) as a hypoxia mimicking agent on human umbilical cord mesenchymal stem cells (hUCMSCs) expression of HIF-1α and mTOR for use in regenerative dentistry. Material and Methods: Human umbilical cord mesenchymal stem cells were isolated and then cultured. The characteristics of stemness were screened and confirmed by flow cytometry. The experiment was conducted on hypoxia (H) and normoxia (N) groups. Each group was divided and incubated into 24-, 48-, and 72-hours observations. Hypoxic treatment was performed using 100 µM CoCl2 on 5th passage cells in a conventional incubator (37°C; 5% CO2). Then, immunofluorescence of HIF-1α and mTOR was done. Data was analyzed statistically using One-way ANOVA and Tukey’s HSD. Results: Significant differences were found between normoxic and hypoxic groups on HIF-1α (p=0.015) and mTOR (p=0.000) expressions. The highest HIF-1α expression was found at 48 hours in the hypoxia group, while for mTOR at 24 hours in the hypoxia group. Conclusion: Hypoxia using cobalt chloride was able to increase human umbilical cord mesenchymal stem cells expression of HIF-1α and mTOR.EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA2024-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.uepb.edu.br/PBOCI/article/view/3123Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 24 (2024); e220128Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 24 (2024); e2201281983-46321519-0501reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)instname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBenghttps://revista.uepb.edu.br/PBOCI/article/view/3123/2245Copyright (c) 2023 Pesquisa Brasileira em Odontopediatria e Clínica Integradahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessKuntjoro, MefinaHendrijantini, NikePrasetyo, Eric PriyoAgustono, BambangSitalaksmi, Ratri MayaHendrianto, ErykDinaryanti, AristikaRusli, MarvinTjendronegoro, EvelynHong, Guang2024-01-17T19:02:51Zoai:ojs.pkp.sfu.ca:article/3123Revistahttps://revista.uepb.edu.br/PBOCI/PUBhttps://revista.uepb.edu.br/PBOCI/oaipboci.editoria@gmail.com || alessandrouepb@gmail.com1983-46321519-0501opendoar:2024-01-17T19:02:51Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)false
dc.title.none.fl_str_mv Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
title Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
spellingShingle Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
Kuntjoro, Mefina
Umbilical Cord
Mesenchymal Stem Cells
Stem Cell Research
Regeneration
title_short Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
title_full Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
title_fullStr Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
title_full_unstemmed Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
title_sort Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
author Kuntjoro, Mefina
author_facet Kuntjoro, Mefina
Hendrijantini, Nike
Prasetyo, Eric Priyo
Agustono, Bambang
Sitalaksmi, Ratri Maya
Hendrianto, Eryk
Dinaryanti, Aristika
Rusli, Marvin
Tjendronegoro, Evelyn
Hong, Guang
author_role author
author2 Hendrijantini, Nike
Prasetyo, Eric Priyo
Agustono, Bambang
Sitalaksmi, Ratri Maya
Hendrianto, Eryk
Dinaryanti, Aristika
Rusli, Marvin
Tjendronegoro, Evelyn
Hong, Guang
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kuntjoro, Mefina
Hendrijantini, Nike
Prasetyo, Eric Priyo
Agustono, Bambang
Sitalaksmi, Ratri Maya
Hendrianto, Eryk
Dinaryanti, Aristika
Rusli, Marvin
Tjendronegoro, Evelyn
Hong, Guang
dc.subject.por.fl_str_mv Umbilical Cord
Mesenchymal Stem Cells
Stem Cell Research
Regeneration
topic Umbilical Cord
Mesenchymal Stem Cells
Stem Cell Research
Regeneration
description Objective: To assess the effects of cobalt chloride (CoCl2) as a hypoxia mimicking agent on human umbilical cord mesenchymal stem cells (hUCMSCs) expression of HIF-1α and mTOR for use in regenerative dentistry. Material and Methods: Human umbilical cord mesenchymal stem cells were isolated and then cultured. The characteristics of stemness were screened and confirmed by flow cytometry. The experiment was conducted on hypoxia (H) and normoxia (N) groups. Each group was divided and incubated into 24-, 48-, and 72-hours observations. Hypoxic treatment was performed using 100 µM CoCl2 on 5th passage cells in a conventional incubator (37°C; 5% CO2). Then, immunofluorescence of HIF-1α and mTOR was done. Data was analyzed statistically using One-way ANOVA and Tukey’s HSD. Results: Significant differences were found between normoxic and hypoxic groups on HIF-1α (p=0.015) and mTOR (p=0.000) expressions. The highest HIF-1α expression was found at 48 hours in the hypoxia group, while for mTOR at 24 hours in the hypoxia group. Conclusion: Hypoxia using cobalt chloride was able to increase human umbilical cord mesenchymal stem cells expression of HIF-1α and mTOR.
publishDate 2024
dc.date.none.fl_str_mv 2024-01-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/3123
url https://revista.uepb.edu.br/PBOCI/article/view/3123
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/3123/2245
dc.rights.driver.fl_str_mv Copyright (c) 2023 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
dc.source.none.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 24 (2024); e220128
Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 24 (2024); e220128
1983-4632
1519-0501
reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
instname:Universidade Estadual da Paraíba (UEPB)
instacron:UEPB
instname_str Universidade Estadual da Paraíba (UEPB)
instacron_str UEPB
institution UEPB
reponame_str Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
collection Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
repository.name.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)
repository.mail.fl_str_mv pboci.editoria@gmail.com || alessandrouepb@gmail.com
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