Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPB |
Texto Completo: | http://tede.bc.uepb.edu.br/jspui/handle/tede/4687 |
Resumo: | Enterolobium contortisiliquum (Vell.) Morong., known as monkfish or monkey, is a legume native to Brazil that occurs from the north to the south of the country. It has a rustic character and adaptability to the edaphoclimatic conditions of the Brazilian semiarid region. Monkfish is a promising species for recovering degraded areas and establishing symbiosis with nitrogen-fixing bacteria. Noduliferous bacteria in legumes are popularly known as rhizobia, in which they are responsible for up to 100% of biological nitrogen fixation. The search for the selection of efficient rhizobia for the production of inoculants appears as an economic, ecological, and sustainable alternative because of the environmental impacts and the high costs of using nitrogen fertilizers. This work aimed to evaluate the genetic diversity of rhizobia isolated from Enterolobium contortisiliquum nodules from soils in the semiarid region of Pernambuco State. Soils were collected in five municipalities of Pernambuco State: Betânia, Parnamirim, Santa Cruz, Santa Maria da Boa Vista, and Serra Talhada. An experiment was set up under nursery conditions to capture the isolates, using monkfish as a trap plant. After 95 days of experimenting, the nodules were collected, and a superficial disinfestation was carried out. The material was spread in streaks to obtain single colonies in Petri dishes with YMA medium. The isolates obtained were genetically characterized by analysis of fragment amplification of the symbiotic genes nifH and nodC using the DuplexPCR, BOX-PCR, ARDRA and partial sequencing of the 16S rRNA gene. A collection of 207 bacteria was obtained from analyzing 127 nodules. Soil from the municipality of Betânia had the highest number of isolates (56), followed by Parnamirim (55), Serra Talhada (43 isolados), Santa Cruz (31) and Santa Maria da Boa Vista (22). In the analysis of genetic variability by BOX-PCR, the monkfish isolates showed different band profiles, demonstrating high genetic diversity. The 32 isolates that were selected for partial sequencing of the 16S rRNA gene were identified in the following genera: Rhizobium (17), Agrobacterium (3), Bradyrhizobium (2), and Paraburkholderia (1). Non-rhizobial isolates belonging to the genera: Pseudomonas (2), Brucella (2), Labrys (2), Pleomorphomonas (1), Bacillus (1), and Siphonobacter (1) were also identified. These results demonstrate that rhizobia isolated from monkfish root nodules from soils in different semiarid regions of Pernambuco State belong to alpha (Rhizobium, Agrobacterium e Bradyrhizobium) and beta-rhizobia (Paraburkholderia). However, there is a predominance of bacteria belonging to the genus Rhizobium, mainly related to the species R. dioscoreae S-93T (99.09 - 100% similarity). |
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Fernandes Júnior, Paulo Ivanhttp://lattes.cnpq.br/5149478274259227Ribeiro, Paula Rose de Almeidahttp://lattes.cnpq.br/2419652337291392Paiva, Iracema de Azevedo Monte2023-07-21T12:33:59Z2999-12-312023-04-14PAIVA, Iracema de Azevedo Monte. Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. 2023. 77 f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2023.http://tede.bc.uepb.edu.br/jspui/handle/tede/4687Enterolobium contortisiliquum (Vell.) Morong., known as monkfish or monkey, is a legume native to Brazil that occurs from the north to the south of the country. It has a rustic character and adaptability to the edaphoclimatic conditions of the Brazilian semiarid region. Monkfish is a promising species for recovering degraded areas and establishing symbiosis with nitrogen-fixing bacteria. Noduliferous bacteria in legumes are popularly known as rhizobia, in which they are responsible for up to 100% of biological nitrogen fixation. The search for the selection of efficient rhizobia for the production of inoculants appears as an economic, ecological, and sustainable alternative because of the environmental impacts and the high costs of using nitrogen fertilizers. This work aimed to evaluate the genetic diversity of rhizobia isolated from Enterolobium contortisiliquum nodules from soils in the semiarid region of Pernambuco State. Soils were collected in five municipalities of Pernambuco State: Betânia, Parnamirim, Santa Cruz, Santa Maria da Boa Vista, and Serra Talhada. An experiment was set up under nursery conditions to capture the isolates, using monkfish as a trap plant. After 95 days of experimenting, the nodules were collected, and a superficial disinfestation was carried out. The material was spread in streaks to obtain single colonies in Petri dishes with YMA medium. The isolates obtained were genetically characterized by analysis of fragment amplification of the symbiotic genes nifH and nodC using the DuplexPCR, BOX-PCR, ARDRA and partial sequencing of the 16S rRNA gene. A collection of 207 bacteria was obtained from analyzing 127 nodules. Soil from the municipality of Betânia had the highest number of isolates (56), followed by Parnamirim (55), Serra Talhada (43 isolados), Santa Cruz (31) and Santa Maria da Boa Vista (22). In the analysis of genetic variability by BOX-PCR, the monkfish isolates showed different band profiles, demonstrating high genetic diversity. The 32 isolates that were selected for partial sequencing of the 16S rRNA gene were identified in the following genera: Rhizobium (17), Agrobacterium (3), Bradyrhizobium (2), and Paraburkholderia (1). Non-rhizobial isolates belonging to the genera: Pseudomonas (2), Brucella (2), Labrys (2), Pleomorphomonas (1), Bacillus (1), and Siphonobacter (1) were also identified. These results demonstrate that rhizobia isolated from monkfish root nodules from soils in different semiarid regions of Pernambuco State belong to alpha (Rhizobium, Agrobacterium e Bradyrhizobium) and beta-rhizobia (Paraburkholderia). However, there is a predominance of bacteria belonging to the genus Rhizobium, mainly related to the species R. dioscoreae S-93T (99.09 - 100% similarity).Enterolobium contortisiliquum (Vell.) Morong., conhecida como tamboril ou orelha de macaco, é uma leguminosa nativa do Brasil que ocorre do norte ao sul do país. Apresenta característica rústica e adaptabilidade para as condições edafoclimáticas do Semiárido brasileiro. Tamboril é uma espécie promissora para a recuperação de áreas degradadas, e estabelece simbiose com bactérias fixadoras de nitrogênio (BFN). As BFN nodulíferas em leguminosas são popularmente conhecidas como rizóbios, no qual, são responsáveis por até 100% da fixação biológica de nitrogênio. A busca pela seleção de rizóbios eficientes, para a produção de inoculantes, surge como uma alternativa econômica, ecológica e sustentável, por causa dos impactos ambientais e dos altos custos no uso de fertilizantes nitrogenados. Esse trabalho teve como objetivo avaliar a diversidade genética de rizóbios isolados de nódulos de Enterolobium contortisiliquum, oriundos de solos do Semiárido pernambucano. Foram coletados solos em cinco municípios de Pernambuco: Betânia, Parnamirim, Santa Cruz, Santa Maria da Boa Vista e Serra Talhada. Para a captura dos isolados, foi montado um experimento, em condições de viveiro, utilizando tamboril como planta-isca. Após 95 dias de condução do experimento os nódulos foram coletados e realizado a desinfestação superficial. Para a obtenção de culturas puras, foramrealizadas sucessivas repicagens em placas de Petri com meio YMA. Os isolados obtidos foram caracterizados geneticamente por meio das análises de amplificação de fragmentos dos genes simbióticos nifH e nodC pela técnica de Duplex-PCR, as técnicas de BOX-PCR e ARDRA e sequenciamento parcial do gene 16S rRNA. Foi obtida uma coleção com 207 isolados bacterianos, a partir da análise de 127 nódulos. O solo oriundo do município de Betânia apresentou a maior quantidade de isolados (56), seguido por Parnamirim (55), Serra Talhada (43 isolados), Santa Cruz (31) e Santa Maria da Boa Vista (22). Na análise da variabilidade genética por BOX-PCR, os isolados de tamboril apresentaram perfis de bandas diversos, demonstrando alta diversidade genética. Os 32 isolados que foram selecionados para o sequenciamento parcial do gene 16S rRNA, foram identificados nos seguintes gêneros: Rhizobium (17), Agrobacterium (3), Bradyrhizobium (2), e Paraburkholderia (1), também foram identificados isolados nãorizobianos, pertencentes aos gêneros: Pseudomonas (2), Brucella (2), Labrys (2), Pleomorphomonas (1), Bacillus (1), e Siphonobacter (1). Esses resultados demonstram que os rizóbios isolados de nódulos radiculares de tamboril, de solos de diferentes localidades do Semiárido pernambucano, pertencem a alfa (Rhizobium, Agrobacterium e Bradyrhizobium) e beta-rizóbios (Paraburkholderia). Porém, ocorre predomínio de bactérias pertencentes ao gênero Rhizobium, principalmente relacionadas a espécie R. dioscoreae S-93T (99,09 - 100% de similaridade)Submitted by Iracema de Azevedo Monte Paiva (iracema.paiva@aluno.uepb.edu.br) on 2023-07-13T17:54:13Z No. of bitstreams: 2 DS-IRACEMA DE AZEVEDO MONTE PAIVA (2).pdf: 1359228 bytes, checksum: ee903b86105b793c8e6bb54a96054adc (MD5) Termo de depósito BDTD.pdf: 845955 bytes, checksum: f06c4b10d085b15a7f9c0d2a4c4527c1 (MD5)Approved for entry into archive by Rosalvo Andrade (rosalvo_andrade@servidor.uepb.edu.br) on 2023-07-14T11:34:48Z (GMT) No. of bitstreams: 2 DS-IRACEMA DE AZEVEDO MONTE PAIVA (2).pdf: 1359228 bytes, checksum: ee903b86105b793c8e6bb54a96054adc (MD5) Termo de depósito BDTD.pdf: 845955 bytes, checksum: f06c4b10d085b15a7f9c0d2a4c4527c1 (MD5)Made available in DSpace on 2023-07-21T12:33:59Z (GMT). No. of bitstreams: 2 DS-IRACEMA DE AZEVEDO MONTE PAIVA (2).pdf: 1359228 bytes, checksum: ee903b86105b793c8e6bb54a96054adc (MD5) Termo de depósito BDTD.pdf: 845955 bytes, checksum: f06c4b10d085b15a7f9c0d2a4c4527c1 (MD5) Previous issue date: 2023-04-14Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Ciências Agrárias - PPGCAUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPTamborilRizóbiosFixação biológica de nitrogênioGene 16S rRNABIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIADiversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong.Diversity of rhizobia native to soils in the semiarid region of Pernambuco isolated from enterolobium contortisiliquum (vell.) morongDiversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) 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dc.title.por.fl_str_mv |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
dc.title.alternative.eng.fl_str_mv |
Diversity of rhizobia native to soils in the semiarid region of Pernambuco isolated from enterolobium contortisiliquum (vell.) morong |
dc.title.alternative.por.fl_str_mv |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
title |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
spellingShingle |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. Paiva, Iracema de Azevedo Monte Tamboril Rizóbios Fixação biológica de nitrogênio Gene 16S rRNA BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
title_short |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
title_full |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
title_fullStr |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
title_full_unstemmed |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
title_sort |
Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. |
author |
Paiva, Iracema de Azevedo Monte |
author_facet |
Paiva, Iracema de Azevedo Monte |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes Júnior, Paulo Ivan |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5149478274259227 |
dc.contributor.referee2.fl_str_mv |
Ribeiro, Paula Rose de Almeida |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2419652337291392 |
dc.contributor.author.fl_str_mv |
Paiva, Iracema de Azevedo Monte |
contributor_str_mv |
Fernandes Júnior, Paulo Ivan Ribeiro, Paula Rose de Almeida |
dc.subject.por.fl_str_mv |
Tamboril Rizóbios Fixação biológica de nitrogênio Gene 16S rRNA |
topic |
Tamboril Rizóbios Fixação biológica de nitrogênio Gene 16S rRNA BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
dc.subject.cnpq.fl_str_mv |
BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
description |
Enterolobium contortisiliquum (Vell.) Morong., known as monkfish or monkey, is a legume native to Brazil that occurs from the north to the south of the country. It has a rustic character and adaptability to the edaphoclimatic conditions of the Brazilian semiarid region. Monkfish is a promising species for recovering degraded areas and establishing symbiosis with nitrogen-fixing bacteria. Noduliferous bacteria in legumes are popularly known as rhizobia, in which they are responsible for up to 100% of biological nitrogen fixation. The search for the selection of efficient rhizobia for the production of inoculants appears as an economic, ecological, and sustainable alternative because of the environmental impacts and the high costs of using nitrogen fertilizers. This work aimed to evaluate the genetic diversity of rhizobia isolated from Enterolobium contortisiliquum nodules from soils in the semiarid region of Pernambuco State. Soils were collected in five municipalities of Pernambuco State: Betânia, Parnamirim, Santa Cruz, Santa Maria da Boa Vista, and Serra Talhada. An experiment was set up under nursery conditions to capture the isolates, using monkfish as a trap plant. After 95 days of experimenting, the nodules were collected, and a superficial disinfestation was carried out. The material was spread in streaks to obtain single colonies in Petri dishes with YMA medium. The isolates obtained were genetically characterized by analysis of fragment amplification of the symbiotic genes nifH and nodC using the DuplexPCR, BOX-PCR, ARDRA and partial sequencing of the 16S rRNA gene. A collection of 207 bacteria was obtained from analyzing 127 nodules. Soil from the municipality of Betânia had the highest number of isolates (56), followed by Parnamirim (55), Serra Talhada (43 isolados), Santa Cruz (31) and Santa Maria da Boa Vista (22). In the analysis of genetic variability by BOX-PCR, the monkfish isolates showed different band profiles, demonstrating high genetic diversity. The 32 isolates that were selected for partial sequencing of the 16S rRNA gene were identified in the following genera: Rhizobium (17), Agrobacterium (3), Bradyrhizobium (2), and Paraburkholderia (1). Non-rhizobial isolates belonging to the genera: Pseudomonas (2), Brucella (2), Labrys (2), Pleomorphomonas (1), Bacillus (1), and Siphonobacter (1) were also identified. These results demonstrate that rhizobia isolated from monkfish root nodules from soils in different semiarid regions of Pernambuco State belong to alpha (Rhizobium, Agrobacterium e Bradyrhizobium) and beta-rhizobia (Paraburkholderia). However, there is a predominance of bacteria belonging to the genus Rhizobium, mainly related to the species R. dioscoreae S-93T (99.09 - 100% similarity). |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-21T12:33:59Z |
dc.date.issued.fl_str_mv |
2023-04-14 |
dc.date.available.fl_str_mv |
2999-12-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PAIVA, Iracema de Azevedo Monte. Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. 2023. 77 f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2023. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4687 |
identifier_str_mv |
PAIVA, Iracema de Azevedo Monte. Diversidade de rizóbios nativos de solos do semiárido pernambucano isolados de Enterolobium contortisiliquum (Vell.) Morong. 2023. 77 f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2023. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4687 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-3549167150673249483 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
524871450381110278 |
dc.relation.cnpq.fl_str_mv |
4689735152161055395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
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