Identificação de uma nova fitase bacteriana através de prospecção metagenômica
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPB |
Texto Completo: | http://tede.bc.uepb.edu.br/jspui/handle/tede/3188 |
Resumo: | The bacterial diversity of environmental samples and their potencial are still poorly explored. Metagenomics approaches offer the opportunity for the prospection of genes with biotechnological interest from non-cultured microorganisms in the laboratory. Materials in the process of composting exhibit high microbiological activity, which indicates that the environment is conducive to the degradation of lignocellulosic material. In this context, several enzymes have been identified by catalyzing these reactions. Phytases are widely produced phosphatase enzymes that hydrolyze phytate by releasing phosphorus and chelated minerals. Due to this potential, these enzymes have become easily interesting for the biotech field. The aim was to investigate the metagenomic potential for the identification of genes with phytase activity. After obtaining the material, DNA extraction was performed, followed by its purification. Total extracted DNA was used to construct the metagenomic library with cosmid vector through commercial kits (EPICENTRE). Positive clones for phytase activity were sequenced and analyzed. The prospection carried out in this study identified a gene (PhyRC001) encoding a phytase where the amino acid identity between PhyRC001 and its nearest published homologs is less than 60%. |
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Meneses, Carlos Henrique Salvino Gadelha03859565419Neder, Diogo Gonçalves04730853644Bonilla, German Andres Estrada70016053443http://lattes.cnpq.br/1156250587826233Farias, Nathálya Carvalho2019-02-18T14:26:02Z2018-02-26FARIAS, N. C. Identificação de uma nova fitase bacteriana através de prospecção metagenômica. 2018. 67f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2018.http://tede.bc.uepb.edu.br/jspui/handle/tede/3188The bacterial diversity of environmental samples and their potencial are still poorly explored. Metagenomics approaches offer the opportunity for the prospection of genes with biotechnological interest from non-cultured microorganisms in the laboratory. Materials in the process of composting exhibit high microbiological activity, which indicates that the environment is conducive to the degradation of lignocellulosic material. In this context, several enzymes have been identified by catalyzing these reactions. Phytases are widely produced phosphatase enzymes that hydrolyze phytate by releasing phosphorus and chelated minerals. Due to this potential, these enzymes have become easily interesting for the biotech field. The aim was to investigate the metagenomic potential for the identification of genes with phytase activity. After obtaining the material, DNA extraction was performed, followed by its purification. Total extracted DNA was used to construct the metagenomic library with cosmid vector through commercial kits (EPICENTRE). Positive clones for phytase activity were sequenced and analyzed. The prospection carried out in this study identified a gene (PhyRC001) encoding a phytase where the amino acid identity between PhyRC001 and its nearest published homologs is less than 60%.A diversidade bacteriana de amostras ambientais e seu potencial ainda são pouco explorados. As abordagens metagenômicas oferecem a oportunidade para a prospecção de genes de interesse biotecnológico a partir de microrganismos não-cultiváveis em laboratório. Materiais em processo de compostagem exibem grande atividade microbiológica, o que torna o ambiente propício para a degradação de material lignocelulósico. Neste contexto, diversas enzimas vêm sendo identificadas por catalisarem estas reações. As fitases são enzimas fosfatases amplamente produzidas que hidrolisam o fitato liberando fósforo e minerais quelados. Devido a este potencial, estas enzimas têm se tornado facilmente interessantes para o campo biotecnológico. Procurou-se assim, investigar o potencial metagenômico para a identificação de genes com atividade fitásica. Após a obtenção do material, foi realizada a extração de DNA, seguida da etapa de purificação. Usou-se o DNA total extraído para construção de biblioteca metagenômica em vetor cosmídeo, através de kits comerciais (EPICENTRE). Os clones positivos para atividade fitásica foram sequenciados e analisados. A prospecção realizada neste estudo, identificou um gene (PhyRC001) que codifica uma fitase, onde, a identidade de aminoácidos entre PhyRC001 e os seus homólogos mais próximos publicados são inferiores a 60%.Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2018-12-03T14:29:23Z No. of bitstreams: 1 PDF - Nathálya Carvalho Farias.pdf: 52901991 bytes, checksum: 8d26d16d185f1620ca5f22dbd3aaac71 (MD5)Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2019-02-18T14:26:02Z (GMT) No. of bitstreams: 1 PDF - Nathálya Carvalho Farias.pdf: 52901991 bytes, checksum: 8d26d16d185f1620ca5f22dbd3aaac71 (MD5)Made available in DSpace on 2019-02-18T14:26:02Z (GMT). No. of bitstreams: 1 PDF - Nathálya Carvalho Farias.pdf: 52901991 bytes, checksum: 8d26d16d185f1620ca5f22dbd3aaac71 (MD5) Previous issue date: 2018-02-26Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.bc.uepb.edu.br/jspui/retrieve/7553/PDF%20-%20Nath%c3%a1lya%20Carvalho%20Farias.pdf.jpgporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Ciências Agrárias - PPGCAUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPOryza sativa L.Restos culturaisMetagenômica funcionalFunctional metagenomicsCultural remainsCIENCIAS AGRARIASIdentificação de uma nova fitase bacteriana através de prospecção metagenômicaIdentification of a new bacterial phytase through metagenomic prospectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-35491671506732494836006006005248714503811102787828424726906663919info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPBinstname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBTHUMBNAILPDF - Nathálya Carvalho Farias.pdf.jpgPDF - Nathálya Carvalho Farias.pdf.jpgimage/jpeg3632http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3188/4/PDF+-+Nath%C3%A1lya+Carvalho+Farias.pdf.jpgaa42d28532b654f1a3431aca2c8a00fcMD54TEXTPDF - Nathálya Carvalho Farias.pdf.txtPDF - Nathálya Carvalho Farias.pdf.txttext/plain69http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3188/3/PDF+-+Nath%C3%A1lya+Carvalho+Farias.pdf.txt75955eca4941f3c38b8b4a7d54057758MD53ORIGINALPDF - Nathálya Carvalho Farias.pdfPDF - Nathálya Carvalho Farias.pdfapplication/pdf52901991http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3188/2/PDF+-+Nath%C3%A1lya+Carvalho+Farias.pdf8d26d16d185f1620ca5f22dbd3aaac71MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81960http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3188/1/license.txt6052ae61e77222b2086e666b7ae213ceMD51tede/31882019-02-19 01:31:10.503oai:tede.bc.uepb.edu.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.bc.uepb.edu.br/jspui/PUBhttp://tede.bc.uepb.edu.br/oai/requestbc@uepb.edu.br||opendoar:2019-02-19T04:31:10Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB)false |
dc.title.por.fl_str_mv |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica |
dc.title.alternative.eng.fl_str_mv |
Identification of a new bacterial phytase through metagenomic prospection |
title |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica |
spellingShingle |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica Farias, Nathálya Carvalho Oryza sativa L. Restos culturais Metagenômica funcional Functional metagenomics Cultural remains CIENCIAS AGRARIAS |
title_short |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica |
title_full |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica |
title_fullStr |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica |
title_full_unstemmed |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica |
title_sort |
Identificação de uma nova fitase bacteriana através de prospecção metagenômica |
author |
Farias, Nathálya Carvalho |
author_facet |
Farias, Nathálya Carvalho |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Meneses, Carlos Henrique Salvino Gadelha |
dc.contributor.advisor1ID.fl_str_mv |
03859565419 |
dc.contributor.referee1.fl_str_mv |
Neder, Diogo Gonçalves |
dc.contributor.referee1ID.fl_str_mv |
04730853644 |
dc.contributor.referee2.fl_str_mv |
Bonilla, German Andres Estrada |
dc.contributor.authorID.fl_str_mv |
70016053443 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1156250587826233 |
dc.contributor.author.fl_str_mv |
Farias, Nathálya Carvalho |
contributor_str_mv |
Meneses, Carlos Henrique Salvino Gadelha Neder, Diogo Gonçalves Bonilla, German Andres Estrada |
dc.subject.por.fl_str_mv |
Oryza sativa L. Restos culturais Metagenômica funcional |
topic |
Oryza sativa L. Restos culturais Metagenômica funcional Functional metagenomics Cultural remains CIENCIAS AGRARIAS |
dc.subject.eng.fl_str_mv |
Functional metagenomics Cultural remains |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
The bacterial diversity of environmental samples and their potencial are still poorly explored. Metagenomics approaches offer the opportunity for the prospection of genes with biotechnological interest from non-cultured microorganisms in the laboratory. Materials in the process of composting exhibit high microbiological activity, which indicates that the environment is conducive to the degradation of lignocellulosic material. In this context, several enzymes have been identified by catalyzing these reactions. Phytases are widely produced phosphatase enzymes that hydrolyze phytate by releasing phosphorus and chelated minerals. Due to this potential, these enzymes have become easily interesting for the biotech field. The aim was to investigate the metagenomic potential for the identification of genes with phytase activity. After obtaining the material, DNA extraction was performed, followed by its purification. Total extracted DNA was used to construct the metagenomic library with cosmid vector through commercial kits (EPICENTRE). Positive clones for phytase activity were sequenced and analyzed. The prospection carried out in this study identified a gene (PhyRC001) encoding a phytase where the amino acid identity between PhyRC001 and its nearest published homologs is less than 60%. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-02-26 |
dc.date.accessioned.fl_str_mv |
2019-02-18T14:26:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FARIAS, N. C. Identificação de uma nova fitase bacteriana através de prospecção metagenômica. 2018. 67f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2018. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3188 |
identifier_str_mv |
FARIAS, N. C. Identificação de uma nova fitase bacteriana através de prospecção metagenômica. 2018. 67f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2018. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3188 |
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por |
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por |
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600 600 600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Estadual da Paraíba |
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Programa de Pós-Graduação em Ciências Agrárias - PPGCA |
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UEPB |
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Brasil |
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Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP |
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Universidade Estadual da Paraíba |
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