Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPB |
Texto Completo: | http://tede.bc.uepb.edu.br/jspui/handle/tede/3993 |
Resumo: | Tacinga inamoena (K. Schum.) N. P. Taylor & Stuppy, known as quipá, is a native cactus Northeast distributed almost everywhere in the semiarid region, it is indicated in folk medicine for diseases in the urethra, asthma, inflammations and combating worms. However, their chemical and pharmacological properties are known. This study aimed to isolate secondary compounds and investigate the biological activities of Tacinga inamoena. The roots was prepared ethanol extract (EEB) and hydroalcoholic extracts (EHA), and performed the physicochemical characterization and thermogravimetric. Isolation and identification of compounds were obtained, respectively, by chromatographic and spectroscopic techniques such as infrared (IR) and Nuclear Magnetic Resonance (NMR) 1H and 13C NMR, using techniques one and two-dimensional beyond comparison with literature data. Antimicrobial activity was tested across bacterial and fungal strains American Type Culture Collection (ATCC) and multiresistant strains of clinical isolates. The minimum inhibitory concentration (MIC) and the ability to modulate the resistance of microbial strains were assessed by broth microdilution method. Cytotoxicity forward the erythrocytes and the antioxidant activity by consumption of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed. In thermogravimetric curve there was the presence of three events related to weight loss, this data standardization work in the plant material, corroborating its quality control. The qualitative screening was suggestive for the presence of alkaloids, flavonoids and tannins. The infrared spectrum of the pulverized material reinforces the roots by the presence of these compounds display characteristic of hydroxyl groups and ester groups. In the semi-quantitative screening yielded a concentration of 65 mg/g polyphenols and 3.3 mg/g of flavonoids in EEB. Five compounds were isolated from the dichloromethane phase (Fdic): β-sitosterol-3- O-glycoside, N-trans feruloyl 4-O methyldopamine, N-cis feruloyl 4-O methyldopamine, N-trans-feruloyl tyramine and N-cis-feruloyl tyramine, all new to the genre. It was observed antimicrobial activity of the ethyl acetate phase (Facet) against the strain of Staphylococcus aureus ATCC (25923) (125 µg/ml) and low antimicrobial activity against multidrug resistant strain of S. aureus 109 (1000 µg/mL). The Fdic, Facet and butanol phase (Fbut) showed activity against Escherichia coli 5I (500 µg/ml) and showed Fdic action also against Escherichia coli 5A (500 µg/mL). The phases partitioned and EEB showed potentiating effect opposite to multiresistant Gram-positive strains (S. aureus 109) and Gram negative (Pseudomonas aeruginosa and E. coli 5A 2) in combination with the tested antibiotics. The β-sitosterol-3-O glycoside showed strong action modulatory front neomycin, ciprofloxacin, norfloxacin, cefazolin and ceftriaxone. The EEB and partitioned phases demonstrated low potential Hemolyzing (<10%) at concentrations that correlated with its biological activity ≤ 500 µg/mL. The EEB showed weak antioxidant activity against the DPPH radical, with IC50 equivalent to 348.66 µg/mL. Tacinga inamoena demonstrated important biological activities and novel compounds in the genus, indicating it as drug production potential candidate. |
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Alves, Harley da Silva03103066473Fechine, Ivana Maria42502608449Castro, Ricardo Dias de03418711413Moura, Ricardo Olímpio de9996321347208105556464Silva, Joanda Paolla Raimundo e2022-01-04T13:12:17Z2016-08-31SILVA, Joanda Paolla Raimundo e. Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy. 2016. 141f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2017.http://tede.bc.uepb.edu.br/jspui/handle/tede/3993Tacinga inamoena (K. Schum.) N. P. Taylor & Stuppy, known as quipá, is a native cactus Northeast distributed almost everywhere in the semiarid region, it is indicated in folk medicine for diseases in the urethra, asthma, inflammations and combating worms. However, their chemical and pharmacological properties are known. This study aimed to isolate secondary compounds and investigate the biological activities of Tacinga inamoena. The roots was prepared ethanol extract (EEB) and hydroalcoholic extracts (EHA), and performed the physicochemical characterization and thermogravimetric. Isolation and identification of compounds were obtained, respectively, by chromatographic and spectroscopic techniques such as infrared (IR) and Nuclear Magnetic Resonance (NMR) 1H and 13C NMR, using techniques one and two-dimensional beyond comparison with literature data. Antimicrobial activity was tested across bacterial and fungal strains American Type Culture Collection (ATCC) and multiresistant strains of clinical isolates. The minimum inhibitory concentration (MIC) and the ability to modulate the resistance of microbial strains were assessed by broth microdilution method. Cytotoxicity forward the erythrocytes and the antioxidant activity by consumption of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed. In thermogravimetric curve there was the presence of three events related to weight loss, this data standardization work in the plant material, corroborating its quality control. The qualitative screening was suggestive for the presence of alkaloids, flavonoids and tannins. The infrared spectrum of the pulverized material reinforces the roots by the presence of these compounds display characteristic of hydroxyl groups and ester groups. In the semi-quantitative screening yielded a concentration of 65 mg/g polyphenols and 3.3 mg/g of flavonoids in EEB. Five compounds were isolated from the dichloromethane phase (Fdic): β-sitosterol-3- O-glycoside, N-trans feruloyl 4-O methyldopamine, N-cis feruloyl 4-O methyldopamine, N-trans-feruloyl tyramine and N-cis-feruloyl tyramine, all new to the genre. It was observed antimicrobial activity of the ethyl acetate phase (Facet) against the strain of Staphylococcus aureus ATCC (25923) (125 µg/ml) and low antimicrobial activity against multidrug resistant strain of S. aureus 109 (1000 µg/mL). The Fdic, Facet and butanol phase (Fbut) showed activity against Escherichia coli 5I (500 µg/ml) and showed Fdic action also against Escherichia coli 5A (500 µg/mL). The phases partitioned and EEB showed potentiating effect opposite to multiresistant Gram-positive strains (S. aureus 109) and Gram negative (Pseudomonas aeruginosa and E. coli 5A 2) in combination with the tested antibiotics. The β-sitosterol-3-O glycoside showed strong action modulatory front neomycin, ciprofloxacin, norfloxacin, cefazolin and ceftriaxone. The EEB and partitioned phases demonstrated low potential Hemolyzing (<10%) at concentrations that correlated with its biological activity ≤ 500 µg/mL. The EEB showed weak antioxidant activity against the DPPH radical, with IC50 equivalent to 348.66 µg/mL. Tacinga inamoena demonstrated important biological activities and novel compounds in the genus, indicating it as drug production potential candidate.Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy, conhecida como quipá, é um cacto nativo da região Nordeste distribuído em quase todo o semiárido, sendo indicado na medicina popular para afecções na uretra, asma, inflamações e no combate a vermes. Entretanto, suas propriedades químicas e farmacológicas são desconhecidas. Este estudo propôs isolar compostos secundários e investigar as atividades biológicas de Tacinga inamoena. Das raízes foi preparado o extrato etanólico bruto (EEB) e os extratos hidroalcoólicos (EHA), e realizado a caracterização físico-química e termogravimétrica. O isolamento e a identificação dos compostos foram obtidos, respectivamente, por técnicas cromatográficas e espectroscópicas, como infravermelho (IV) e Ressonância Magnética Nuclear (RMN) de 1H e 13C, utilizando técnicas uni e bidimensionais, além da comparação com dados da literatura. A atividade antimicrobiana foi testada frente às cepas bacterianas e fúngicas American Type Culture Collection (ATCC) e cepas multiresistentes de isolados clínicos. A concentração inibitória mínima (CIM) e a capacidade de modular a resistência das cepas microbianas foram avaliadas pelo método de microdiluição. Foi realizada a citotoxicidade frente a eritrócitos e a atividade antioxidante por consumo do 2,2-difenil-1-picrilhidrazil (DPPH). Na curva termogravimétrica verificou-se a presença de três eventos relativos à perda de massa, estes dados atuam na padronização do material vegetal, corroborando com seu controle de qualidade. O screening qualitativo foi sugestivo para presença de alcaloides, flavonoides e taninos. O espectro de infravermelho do material pulverizado das raízes reforça a presença destes compostos por apresentar grupamentos característicos de grupos hidroxila e éster. No screening semi quantitativo obteve-se uma concentração de 65 mg/g de polifenóis e 3,3 mg/g de flavonoides no EEB. Foram isolados cinco compostos, a partir da fase diclorometano (Fdic): β-sitosterol-3-O-glicosideo, N-trans-feruloyl-4-O-metildopamina, N-cis-feruloyl 4-O-metildopamina N-trans-feruloyl tiramina e N-cis-feruloyl tiramina, todos inéditos no gênero e na espécie. Foi observada atividade antimicrobiana da fase acetato de etila (Facet) frente à cepa de Staphylococcus aureus ATCC (25923) (125 µg/mL) e fraca atividade antimicrobiana frente a cepa multiresistente de S. aureus 109 (1000 µg/mL). As Fdic, Facet e fase butanólica (Fbut) apresentaram ação frente a Escherichia coli 5I (500 µg/mL) e a Fdic apresentou ação também contra Escherichia coli 5A (500 µg/mL). O EEB e fases particionadas apresentaram efeito potencializador frente às cepas multirresistentes Gram positivas (S. aureus 109) e Gram negativas (Pseudomonas aeruginosa 2 e E. coli 5A) em combinação com os antimicrobianos testados. O β-sitosterol-3-O-glicosideo apresentou forte ação moduladora frente a neomicina, ciprofloxacino, norfloxacino, cefazolina e ceftriaxona. O EEB e as fases particionadas demonstraram baixo potencial hemolisante (<10%) nas concentrações que se correlacionaram com sua atividade biológica ≤ 500 µg/mL. O EEB apresentou fraca atividade antioxidante frente ao radical DPPH, com IC50 equivalente a 348.66 µg/mL. Tacinga inamoena demonstrou importantes atividades biológicas e compostos inéditos no gênero, indicando-a como potencial candidato a produção de medicamentos.Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2021-12-17T11:54:31Z No. of bitstreams: 1 PDF - Joanda Paolla Raimundo e Silva.pdf: 5821336 bytes, checksum: 7e194fa66be65579a60adb6bee314e51 (MD5)Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2022-01-04T13:08:22Z (GMT) No. of bitstreams: 1 PDF - Joanda Paolla Raimundo e Silva.pdf: 5821336 bytes, checksum: 7e194fa66be65579a60adb6bee314e51 (MD5)Made available in DSpace on 2022-01-04T13:12:17Z (GMT). No. of bitstreams: 1 PDF - Joanda Paolla Raimundo e Silva.pdf: 5821336 bytes, checksum: 7e194fa66be65579a60adb6bee314e51 (MD5) Previous issue date: 2016-08-31Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Ciências Farmacêuticas - PPGCFUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPCactaceaeQuipáEtnofarmacologiaAtividade biológicaCactaceaeQuipaCIENCIAS DA SAUDE::FARMACIAEstudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. 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dc.title.por.fl_str_mv |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy |
title |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy |
spellingShingle |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy Silva, Joanda Paolla Raimundo e Cactaceae Quipá Etnofarmacologia Atividade biológica Cactaceae Quipa CIENCIAS DA SAUDE::FARMACIA |
title_short |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy |
title_full |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy |
title_fullStr |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy |
title_full_unstemmed |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy |
title_sort |
Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy |
author |
Silva, Joanda Paolla Raimundo e |
author_facet |
Silva, Joanda Paolla Raimundo e |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Alves, Harley da Silva |
dc.contributor.advisor1ID.fl_str_mv |
03103066473 |
dc.contributor.advisor-co1.fl_str_mv |
Fechine, Ivana Maria |
dc.contributor.advisor-co1ID.fl_str_mv |
42502608449 |
dc.contributor.referee1.fl_str_mv |
Castro, Ricardo Dias de |
dc.contributor.referee1ID.fl_str_mv |
03418711413 |
dc.contributor.referee2.fl_str_mv |
Moura, Ricardo Olímpio de |
dc.contributor.referee2ID.fl_str_mv |
99963213472 |
dc.contributor.authorID.fl_str_mv |
08105556464 |
dc.contributor.author.fl_str_mv |
Silva, Joanda Paolla Raimundo e |
contributor_str_mv |
Alves, Harley da Silva Fechine, Ivana Maria Castro, Ricardo Dias de Moura, Ricardo Olímpio de |
dc.subject.por.fl_str_mv |
Cactaceae Quipá Etnofarmacologia Atividade biológica |
topic |
Cactaceae Quipá Etnofarmacologia Atividade biológica Cactaceae Quipa CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Cactaceae Quipa |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA |
description |
Tacinga inamoena (K. Schum.) N. P. Taylor & Stuppy, known as quipá, is a native cactus Northeast distributed almost everywhere in the semiarid region, it is indicated in folk medicine for diseases in the urethra, asthma, inflammations and combating worms. However, their chemical and pharmacological properties are known. This study aimed to isolate secondary compounds and investigate the biological activities of Tacinga inamoena. The roots was prepared ethanol extract (EEB) and hydroalcoholic extracts (EHA), and performed the physicochemical characterization and thermogravimetric. Isolation and identification of compounds were obtained, respectively, by chromatographic and spectroscopic techniques such as infrared (IR) and Nuclear Magnetic Resonance (NMR) 1H and 13C NMR, using techniques one and two-dimensional beyond comparison with literature data. Antimicrobial activity was tested across bacterial and fungal strains American Type Culture Collection (ATCC) and multiresistant strains of clinical isolates. The minimum inhibitory concentration (MIC) and the ability to modulate the resistance of microbial strains were assessed by broth microdilution method. Cytotoxicity forward the erythrocytes and the antioxidant activity by consumption of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed. In thermogravimetric curve there was the presence of three events related to weight loss, this data standardization work in the plant material, corroborating its quality control. The qualitative screening was suggestive for the presence of alkaloids, flavonoids and tannins. The infrared spectrum of the pulverized material reinforces the roots by the presence of these compounds display characteristic of hydroxyl groups and ester groups. In the semi-quantitative screening yielded a concentration of 65 mg/g polyphenols and 3.3 mg/g of flavonoids in EEB. Five compounds were isolated from the dichloromethane phase (Fdic): β-sitosterol-3- O-glycoside, N-trans feruloyl 4-O methyldopamine, N-cis feruloyl 4-O methyldopamine, N-trans-feruloyl tyramine and N-cis-feruloyl tyramine, all new to the genre. It was observed antimicrobial activity of the ethyl acetate phase (Facet) against the strain of Staphylococcus aureus ATCC (25923) (125 µg/ml) and low antimicrobial activity against multidrug resistant strain of S. aureus 109 (1000 µg/mL). The Fdic, Facet and butanol phase (Fbut) showed activity against Escherichia coli 5I (500 µg/ml) and showed Fdic action also against Escherichia coli 5A (500 µg/mL). The phases partitioned and EEB showed potentiating effect opposite to multiresistant Gram-positive strains (S. aureus 109) and Gram negative (Pseudomonas aeruginosa and E. coli 5A 2) in combination with the tested antibiotics. The β-sitosterol-3-O glycoside showed strong action modulatory front neomycin, ciprofloxacin, norfloxacin, cefazolin and ceftriaxone. The EEB and partitioned phases demonstrated low potential Hemolyzing (<10%) at concentrations that correlated with its biological activity ≤ 500 µg/mL. The EEB showed weak antioxidant activity against the DPPH radical, with IC50 equivalent to 348.66 µg/mL. Tacinga inamoena demonstrated important biological activities and novel compounds in the genus, indicating it as drug production potential candidate. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-08-31 |
dc.date.accessioned.fl_str_mv |
2022-01-04T13:12:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Joanda Paolla Raimundo e. Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy. 2016. 141f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2017. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3993 |
identifier_str_mv |
SILVA, Joanda Paolla Raimundo e. Estudo fitoquímico e atividade biológica da Tacinga inamoena (K. Schum.) N.P. Taylor & Stuppy. 2016. 141f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2017. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3993 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
8157968555251876874 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
524871450381110278 |
dc.relation.cnpq.fl_str_mv |
6997636413449754996 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual da Paraíba |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF |
dc.publisher.initials.fl_str_mv |
UEPB |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP |
publisher.none.fl_str_mv |
Universidade Estadual da Paraíba |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEPB instname:Universidade Estadual da Paraíba (UEPB) instacron:UEPB |
instname_str |
Universidade Estadual da Paraíba (UEPB) |
instacron_str |
UEPB |
institution |
UEPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEPB |
collection |
Biblioteca Digital de Teses e Dissertações da UEPB |
bitstream.url.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3993/2/PDF+-+Joanda+Paolla+Raimundo+e+Silva.pdf http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3993/1/license.txt |
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7e194fa66be65579a60adb6bee314e51 6052ae61e77222b2086e666b7ae213ce |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB) |
repository.mail.fl_str_mv |
bc@uepb.edu.br|| |
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1800227423307956224 |