Cultura de tecidos de Euphorbia heterophylla L
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPG |
Texto Completo: | http://tede2.uepg.br/jspui/handle/prefix/2240 |
Resumo: | The general objective of this research was the evaluation of in vitro culture of Euphorbia heterophylla L. one of the principal weeds that effects yield of important economic crops such as soybean and corn. In addition, some assays considered the evaluation of the species’ potential biochemical value to the pharmaceutical industry. In vitro germination of E. heterophylla seed was tested on cotton batting as substrate, full strength and ½ MS basal medium. Results show a greater percent germination and better growth indices of plant development on the ½ MS medium. Explants tested for organogenesis included hypocotyls, cotyledon and root explants which were plated on media containing the following plant growth regulators (phytohormones): 0.5 mg/L 2ip, 1mg/L Kin + 0.1 mg/L IAA, 0.5 mg/L 2ip + 0.1 mg/L IAA, 1mg/L 2ip + 0.1mg IAA, 1.5 mg.L-1 2iP + 0.1 mg.L-1 AIA added to ½ MS media. Explants were evaluated for the presence of buds, the number of buds/ explant and the number of plantlets that were rooted and re-established. On the average, hypocotyls explants responded with two buds/ explant and 50% of these developed by direct organogenesis on media containing 0.5 mg/L of 2ip. No regeneration was observed on cotyledon or root explants. Root cultures developed in all treatments with or without auxin. These results will assist in future physiological tests such as evaluating the herbicide resistance of this species and also in evaluating possible use in the pharmaceutical industry. With this objective in mind we tested the sensitivity of root cultures in vitro. Roots were placed in ½ MS media containing 0,1,2,4 and 8 times the manufacturer’s recommended dose for the products Scepter® and Flex®. The active ingredients were imazaquin and fomesafen respectively, and they are inhibitors of ALS synthesis and PROTOX. The results obtained showed evidence of suppression of the normal growth of these roots as compared to the control treatment. Therefore, this suggests that an in vitro test of this type can be used to measure resistance this species to herbicides. |
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Ayub, Ricardo AntonioCPF:54603722672http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782860Z5Quoirin, Marguerite Germaine GhislaineCPF:02101926741http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780001Z7Biasi, Luiz AntonioCPF:69177309987http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782461A3CPF:00598392955http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4758374Z0Colussi, Francieli2017-07-25T19:29:59Z2006-12-202017-07-25T19:29:59Z2006-09-01COLUSSI, Francieli. Cultura de tecidos de Euphorbia heterophylla L. 2006. 59 f. Dissertação (Mestrado em Agricultura) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2006.http://tede2.uepg.br/jspui/handle/prefix/2240The general objective of this research was the evaluation of in vitro culture of Euphorbia heterophylla L. one of the principal weeds that effects yield of important economic crops such as soybean and corn. In addition, some assays considered the evaluation of the species’ potential biochemical value to the pharmaceutical industry. In vitro germination of E. heterophylla seed was tested on cotton batting as substrate, full strength and ½ MS basal medium. Results show a greater percent germination and better growth indices of plant development on the ½ MS medium. Explants tested for organogenesis included hypocotyls, cotyledon and root explants which were plated on media containing the following plant growth regulators (phytohormones): 0.5 mg/L 2ip, 1mg/L Kin + 0.1 mg/L IAA, 0.5 mg/L 2ip + 0.1 mg/L IAA, 1mg/L 2ip + 0.1mg IAA, 1.5 mg.L-1 2iP + 0.1 mg.L-1 AIA added to ½ MS media. Explants were evaluated for the presence of buds, the number of buds/ explant and the number of plantlets that were rooted and re-established. On the average, hypocotyls explants responded with two buds/ explant and 50% of these developed by direct organogenesis on media containing 0.5 mg/L of 2ip. No regeneration was observed on cotyledon or root explants. Root cultures developed in all treatments with or without auxin. These results will assist in future physiological tests such as evaluating the herbicide resistance of this species and also in evaluating possible use in the pharmaceutical industry. With this objective in mind we tested the sensitivity of root cultures in vitro. Roots were placed in ½ MS media containing 0,1,2,4 and 8 times the manufacturer’s recommended dose for the products Scepter® and Flex®. The active ingredients were imazaquin and fomesafen respectively, and they are inhibitors of ALS synthesis and PROTOX. The results obtained showed evidence of suppression of the normal growth of these roots as compared to the control treatment. Therefore, this suggests that an in vitro test of this type can be used to measure resistance this species to herbicides.O objetivo geral deste trabalho foi estudar o cultivo in vitro de Euphorbia heterophylla. L, que é uma das principais plantas daninhas que afeta a produtividade das grandes culturas como soja e milho. Essa espécie também vem sendo estudado pelos aspectos fitoquímicos para indústria farmacêutica, para isto foram propostos alguns ensaios. Para avaliar a germinação in vitro de sementes de E. heterophylla foram testados substratos de algodão, meio MS e MS/2. Os resultados mostraram maior porcentagem de germinação e melhores índices de desenvolvimento das plantas em meio MS/2. Para o teste de organogênese foram utilizados explantes hipocotiledonares, cotiledonares e radiculares com as seguintes tratamentos: 0,5 mg.L-1 2iP, 1mg.L-1 CIN + 0,1 mg.L-1 AIA, 0,5 mg.L-1 2iP + 0,1 mg.L-1 AIA, 1 mg.L-1 2iP + 0,1 mg.L-1 AIA , 1,5 mg.L-1 2iP + 0,1 mg.L-1 AIA adicionado ao meio MS/2. Foram avaliados o número de explantes com gema, número de gemas por explante, número de plântulas enraizadas e aclimatadas. Obteve-se em média duas gemas por explante hipocotiledonar, em 50% destes, a partir de organogênese direta com o uso de 0,5 mg.L-1 de 2iP. Não houve regeneração a partir dos explantes cotiledonares e radiculares. A cultura racinar desenvolveu-se em todos os tratamentos contendo ou não auxina. Com o objetivo de avaliar a resistência desta planta a herbicidas, testamos a sensibilidade de culturas de raízes in vitro. As raízes foram repicadas em meio MS/2 com as seguintes concentrações: 0; 1; 2; 4 e 8 vezes a dose recomendada pelo fabricante dos herbicidas com ingrediente ativo imazaquin o fomesafen, inibidores da síntese de ALS e PROTOX. Com nomes comerciais de Scepter® e Flex®, respectivamente. Os resultados obtidos evidenciaram um retardo no crescimento normal destas raízes, quando comparada a testemunha.Made available in DSpace on 2017-07-25T19:29:59Z (GMT). No. of bitstreams: 1 francielicolussi.pdf: 2001927 bytes, checksum: 373492674f45bb0ed7d467ad407070d5 (MD5) Previous issue date: 2006-09-01application/pdfporUNIVERSIDADE ESTADUAL DE PONTA GROSSAPrograma de Pós-Graduação em AgronomiaUEPGBRAgriculturaleiteirocultura de tecidosorganogênesewild posentiatissue cultureorganogenesisCNPQ::CIENCIAS AGRARIAS::AGRONOMIACultura de tecidos de Euphorbia heterophylla Linfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPGinstname:Universidade Estadual de Ponta Grossa (UEPG)instacron:UEPGORIGINALfrancielicolussi.pdfapplication/pdf2001927http://tede2.uepg.br/jspui/bitstream/prefix/2240/1/francielicolussi.pdf373492674f45bb0ed7d467ad407070d5MD51prefix/22402017-07-25 16:29:59.595oai:tede2.uepg.br:prefix/2240Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.uepg.br/jspui/PUBhttp://tede2.uepg.br/oai/requestbicen@uepg.br||mv_fidelis@yahoo.com.bropendoar:2017-07-25T19:29:59Biblioteca Digital de Teses e Dissertações da UEPG - Universidade Estadual de Ponta Grossa (UEPG)false |
dc.title.por.fl_str_mv |
Cultura de tecidos de Euphorbia heterophylla L |
title |
Cultura de tecidos de Euphorbia heterophylla L |
spellingShingle |
Cultura de tecidos de Euphorbia heterophylla L Colussi, Francieli leiteiro cultura de tecidos organogênese wild posentia tissue culture organogenesis CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Cultura de tecidos de Euphorbia heterophylla L |
title_full |
Cultura de tecidos de Euphorbia heterophylla L |
title_fullStr |
Cultura de tecidos de Euphorbia heterophylla L |
title_full_unstemmed |
Cultura de tecidos de Euphorbia heterophylla L |
title_sort |
Cultura de tecidos de Euphorbia heterophylla L |
author |
Colussi, Francieli |
author_facet |
Colussi, Francieli |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ayub, Ricardo Antonio |
dc.contributor.advisor1ID.fl_str_mv |
CPF:54603722672 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782860Z5 |
dc.contributor.referee1.fl_str_mv |
Quoirin, Marguerite Germaine Ghislaine |
dc.contributor.referee1ID.fl_str_mv |
CPF:02101926741 |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780001Z7 |
dc.contributor.referee2.fl_str_mv |
Biasi, Luiz Antonio |
dc.contributor.referee2ID.fl_str_mv |
CPF:69177309987 |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782461A3 |
dc.contributor.authorID.fl_str_mv |
CPF:00598392955 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4758374Z0 |
dc.contributor.author.fl_str_mv |
Colussi, Francieli |
contributor_str_mv |
Ayub, Ricardo Antonio Quoirin, Marguerite Germaine Ghislaine Biasi, Luiz Antonio |
dc.subject.por.fl_str_mv |
leiteiro cultura de tecidos organogênese |
topic |
leiteiro cultura de tecidos organogênese wild posentia tissue culture organogenesis CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
wild posentia tissue culture organogenesis |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The general objective of this research was the evaluation of in vitro culture of Euphorbia heterophylla L. one of the principal weeds that effects yield of important economic crops such as soybean and corn. In addition, some assays considered the evaluation of the species’ potential biochemical value to the pharmaceutical industry. In vitro germination of E. heterophylla seed was tested on cotton batting as substrate, full strength and ½ MS basal medium. Results show a greater percent germination and better growth indices of plant development on the ½ MS medium. Explants tested for organogenesis included hypocotyls, cotyledon and root explants which were plated on media containing the following plant growth regulators (phytohormones): 0.5 mg/L 2ip, 1mg/L Kin + 0.1 mg/L IAA, 0.5 mg/L 2ip + 0.1 mg/L IAA, 1mg/L 2ip + 0.1mg IAA, 1.5 mg.L-1 2iP + 0.1 mg.L-1 AIA added to ½ MS media. Explants were evaluated for the presence of buds, the number of buds/ explant and the number of plantlets that were rooted and re-established. On the average, hypocotyls explants responded with two buds/ explant and 50% of these developed by direct organogenesis on media containing 0.5 mg/L of 2ip. No regeneration was observed on cotyledon or root explants. Root cultures developed in all treatments with or without auxin. These results will assist in future physiological tests such as evaluating the herbicide resistance of this species and also in evaluating possible use in the pharmaceutical industry. With this objective in mind we tested the sensitivity of root cultures in vitro. Roots were placed in ½ MS media containing 0,1,2,4 and 8 times the manufacturer’s recommended dose for the products Scepter® and Flex®. The active ingredients were imazaquin and fomesafen respectively, and they are inhibitors of ALS synthesis and PROTOX. The results obtained showed evidence of suppression of the normal growth of these roots as compared to the control treatment. Therefore, this suggests that an in vitro test of this type can be used to measure resistance this species to herbicides. |
publishDate |
2006 |
dc.date.available.fl_str_mv |
2006-12-20 2017-07-25T19:29:59Z |
dc.date.issued.fl_str_mv |
2006-09-01 |
dc.date.accessioned.fl_str_mv |
2017-07-25T19:29:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COLUSSI, Francieli. Cultura de tecidos de Euphorbia heterophylla L. 2006. 59 f. Dissertação (Mestrado em Agricultura) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2006. |
dc.identifier.uri.fl_str_mv |
http://tede2.uepg.br/jspui/handle/prefix/2240 |
identifier_str_mv |
COLUSSI, Francieli. Cultura de tecidos de Euphorbia heterophylla L. 2006. 59 f. Dissertação (Mestrado em Agricultura) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2006. |
url |
http://tede2.uepg.br/jspui/handle/prefix/2240 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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UNIVERSIDADE ESTADUAL DE PONTA GROSSA |
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Programa de Pós-Graduação em Agronomia |
dc.publisher.initials.fl_str_mv |
UEPG |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Agricultura |
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UNIVERSIDADE ESTADUAL DE PONTA GROSSA |
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